TY  - JOUR
A1  - Olejko, Lydia
A1  - Cywiński, Piotr J.
A1  - Bald, Ilko
T1  - An ion-controlled four-color fluorescent telomeric switch on DNA origami structures
JF  - Nanoscale
N2  - The folding of single-stranded telomeric DNA into guanine (G) quadruplexes is a conformational change that plays a major role in sensing and drug targeting. The telomeric DNA can be placed on DNA origami nanostructures to make the folding process extremely selective for K+ ions even in the presence of high Na+ concentrations. Here, we demonstrate that the K+-selective G-quadruplex formation is reversible when using a cryptand to remove K+ from the G-quadruplex. We present a full characterization of the reversible switching between single-stranded telomeric DNA and G-quadruplex structures using Förster resonance energy transfer (FRET) between the dyes fluorescein (FAM) and cyanine3 (Cy3). When attached to the DNA origami platform, the G-quadruplex switch can be incorporated into more complex photonic networks, which is demonstrated for a three-color and a four-color FRET cascade from FAM over Cy3 and Cy5 to IRDye700 with G-quadruplex-Cy3 acting as a switchable transmitter.
KW  - resonance energy-transfer
KW  - g-quadruplex
KW  - quantum dots
KW  - strand breakage
KW  - photonic wires
KW  - 3-color fret
KW  - nanostructures
KW  - recognition
KW  - sensitivity
KW  - assemblies
Y1  - 2016
U6  - https://doi.org/10.1039/C6NR00119J
SN  - 2040-3372
SN  - 2040-3364
VL  - 8
SP  - 10339
EP  - 10347
PB  - RSC Publ.
CY  - Cambridge
ER  - 
TY  - GEN
A1  - Olejko, Lydia
A1  - Cywiński, P. J.
A1  - Bald, Ilko
T1  - An ion-controlled four-color fluorescent telomeric switch on DNA origami structures
N2  - The folding of single-stranded telomeric DNA into guanine (G) quadruplexes is a conformational change that plays a major role in sensing and drug targeting. The telomeric DNA can be placed on DNA origami nanostructures to make the folding process extremely selective for K+ ions even in the presence of high Na+ concentrations. Here, we demonstrate that the K+-selective G-quadruplex formation is reversible when using a cryptand to remove K+ from the G-quadruplex. We present a full characterization of the reversible switching between single-stranded telomeric DNA and G-quadruplex structures using Förster resonance energy transfer (FRET) between the dyes fluorescein (FAM) and cyanine3 (Cy3). When attached to the DNA origami platform, the G-quadruplex switch can be incorporated into more complex photonic networks, which is demonstrated for a three-color and a four-color FRET cascade from FAM over Cy3 and Cy5 to IRDye700 with G-quadruplex-Cy3 acting as a switchable transmitter.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 254 
KW  - resonance energy-transfer
KW  - g-quadruplex
KW  - quantum dots
KW  - strand breakage
KW  - photonic wires
KW  - 3-color fret
KW  - nanostructures
KW  - recognition
KW  - sensitivity
KW  - assemblies
Y1  - 2016
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-95831
SP  - 10339
EP  - 10347
ER  -