TY  - JOUR
A1  - Bartholomäus, Alexander
A1  - Fedyunin, Ivan
A1  - Feist, Peter
A1  - Sin, Celine
A1  - Zhang, Gong
A1  - Valleriani, Angelo
A1  - Ignatova, Zoya
T1  - Bacteria differently regulate mRNA abundance to specifically respond to various stresses
JF  - Geology
N2  - Environmental stress is detrimental to cell viability and requires an adequate reprogramming of cellular activities to maximize cell survival. We present a global analysis of the response of Escherichia coli to acute heat and osmotic stress. We combine deep sequencing of total mRNA and ribosome-protected fragments to provide a genome-wide map of the stress response at transcriptional and translational levels. For each type of stress, we observe a unique subset of genes that shape the stress-specific response. Upon temperature upshift, mRNAs with reduced folding stability up-and downstream of the start codon, and thus with more accessible initiation regions, are translationally favoured. Conversely, osmotic upshift causes a global reduction of highly translated transcripts with high copy numbers, allowing reallocation of translation resources to not degraded and newly synthesized mRNAs.
KW  - transcription
KW  - translation
KW  - deep sequencing
KW  - Escherichia coli
KW  - copy numbers
Y1  - 2016
U6  - https://doi.org/10.1098/rsta.2015.0069
SN  - 1364-503X
SN  - 1471-2962
VL  - 374
PB  - Royal Society
CY  - London
ER  -