TY  - JOUR
A1  - Hardy, John G.
A1  - Torres-Rendon, Jose Guillermo
A1  - Leal-Egana, Aldo
A1  - Walther, Andreas
A1  - Schlaad, Helmut
A1  - Coelfen, Helmut
A1  - Scheibel, Thomas R.
T1  - Biomineralization of Engineered Spider Silk Protein-Based Composite Materials for Bone Tissue Engineering
JF  - Materials
N2  - Materials based on biodegradable polyesters, such as poly(butylene terephthalate) (PBT) or poly(butylene terephthalate-co-poly(alkylene glycol) terephthalate) (PBTAT), have potential application as pro-regenerative scaffolds for bone tissue engineering. Herein, the preparation of films composed of PBT or PBTAT and an engineered spider silk protein, (eADF4(C16)), that displays multiple carboxylic acid moieties capable of binding calcium ions and facilitating their biomineralization with calcium carbonate or calcium phosphate is reported. Human mesenchymal stem cells cultured on films mineralized with calcium phosphate show enhanced levels of alkaline phosphatase activity suggesting that such composites have potential use for bone tissue engineering.
KW  - spider silk
KW  - recombinant protein
KW  - biodegradable polymers
KW  - biomaterials
KW  - biomineralization
KW  - bone tissue engineering
Y1  - 2016
U6  - https://doi.org/10.3390/ma9070560
SN  - 1996-1944
VL  - 9
SP  - 93
EP  - 108
PB  - MDPI
CY  - Basel
ER  -