TY  - JOUR
A1  - Dongmo, Saustin
A1  - Leyk, Janina
A1  - Dosche, Carsten
A1  - Richter-Landsberg, Christiane
A1  - Wollenberger, Ursula
A1  - Wittstock, Gunther
T1  - Electrogeneration of O-2(center dot-) and H2O2 Using Polymer-modified Microelectrodes in the Environment of Living Cells
JF  - Electroanalysis : an international journal devoted to fundamental and practical aspects of electroanalysis
N2  - Microelectrodes modified with electropolymerized plumbagin (PLG) were used for the generation of superoxide radical (O-2(center dot-)) and hydrogen peroxide (H2O2) during oxygen reduction reaction (ORR) in an aqueous medium, specifically in serum-free cell culture media. This is enabled by the specific design of a polymer film on the microelectrode. The generation and diffusion of O-2(center dot-) during electrocatalytic ORR at a positionable PLG polymer-modified microelectrode was followed by fluorescence microscopy with the selective dye 4-chloro-7-nitrobenzo-2-oxa-1,3-diazole (NBD-Cl) and by amperometric detection using a cytochrome c-modified electrode at + 0.13 V. H2O2 production, either by direct oxygen reduction or as product of O-2(center dot-) disproportionation, was monitored by the reaction with Amplex UltraRed. The PLG polymer-modified microelectrodes were used to expose mammalian B6-RPE07 retinal cells to defined local fluxes of reactive oxygen species (ROS), and cellular responses and morphological alterations were observed. The use of a controllable source of ROS opens many possibilities to study how living cells respond to the presence of a certain flux of specific ROS.
KW  - reactive oxygen species
KW  - microelectrode
KW  - scanning electrochemical microscopy
KW  - biosensor
KW  - polymer-modified electrode
KW  - oxygen reduction reaction
Y1  - 2016
U6  - https://doi.org/10.1002/elan.201600267
SN  - 1040-0397
SN  - 1521-4109
VL  - 28
SP  - 2400
EP  - 2407
PB  - Wiley-VCH
CY  - Weinheim
ER  -