TY  - JOUR
A1  - Sun, Jing
A1  - Cernoch, Peter
A1  - Völkel, Antje
A1  - Wei, Yuhan
A1  - Ruokolainen, Janne
A1  - Schlaad, Helmut
T1  - Aqueous Self-Assembly of a Protein-Mimetic Ampholytic Block Copolypeptide
JF  - Macromolecules : a publication of the American Chemical Society
N2  - This report describes the aggregation behavior of an ABC-type ampholytic block copolypeptide, poly(ethylene oxide)-block-poly(L-lysine)-block-poly(L-glutamate), in aqueous media in dependence of pH. Polypeptide secondary structures and self-assemblies are investigated by circular dichroism (CD), Fourier transform infrared (FT-IR) and NMR spectroscopy, zeta potential measurements, analytical ultracentrifugation (AUC), dynamic/static light scattering (DLS/SLS), and cryogenic transmission electron microscopy (cryoTEM). The polymer chains tend to form vesicles when the hydrophobic polypeptide helix is located at the chain end (acidic pH) and are existing as single chains when it is located in the center and flanked by the two hydrophilic segments (basic pH). Precipitation occurs in the intermediate pH range due to polyion complexation of the charged polypeptide segments.
Y1  - 2016
U6  - https://doi.org/10.1021/acs.macromol.6b00817
SN  - 0024-9297
SN  - 1520-5835
VL  - 49
SP  - 5494
EP  - 5501
PB  - American Chemical Society
CY  - Washington
ER  -