TY - GEN A1 - Morris, Penelope J. A1 - Salt, Carina A1 - Raila, Jens A1 - Brenten, Thomas A1 - Kohn, Barbara A1 - Schweigert, Florian J. A1 - Zentek, Jürgen T1 - Safety evaluation of vitamin A in growing dogs T2 - Potsprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe N2 - The safe upper limit for inclusion of vitamin A in complete diets for growing dogs is uncertain, with the result that current recommendations range from 5.24 to 104.80 mu mol retinol (5000 to 100 000 IU vitamin A)/4184 kJ (1000 kcal) metabolisable energy (ME). The aim of the present study was to determine the effect of feeding four concentrations of vitamin A to puppies from weaning until 1 year of age. A total of forty-nine puppies, of two breeds, Labrador Retriever and Miniature Schnauzer, were randomly assigned to one of four treatment groups. Following weaning at 8 weeks of age, puppies were fed a complete food supplemented with retinyl acetate diluted in vegetable oil and fed at 1ml oil/100 g diet to achieve an intake of 5.24, 13.10, 78.60 and 104.80 mu mol retinol (5000, 12 500, 75 000 and 100 000 IU vitamin A)/4184 kJ (1000 kcal) ME. Fasted blood and urine samples were collected at 8, 10, 12, 14, 16, 20, 26, 36 and 52 weeks of age and analysed for markers of vitamin A metabolism and markers of safety including haematological and biochemical variables, bone-specific alkaline phosphatase, cross-linked carboxyterminal telopeptides of type I collagen and dual-energy X-ray absorptiometry. Clinical examinations were conducted every 4 weeks. Data were analysed by means of a mixed model analysis with Bonferroni corrections for multiple endpoints. There was no effect of vitamin A concentration on any of the parameters, with the exception of total serum retinyl esters, and no effect of dose on the number, type and duration of adverse events. We therefore propose that 104.80 mu mol retinol (100 000 IU vitamin A)/4184 kJ (1000 kcal) is a suitable safe upper limit for use in the formulation of diets designed for puppy growth. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 686 KW - puppies KW - dogs KW - Retinol KW - Retinyl esters KW - vitamin A Y1 - 2019 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-414929 IS - 686 ER - TY - CHAP A1 - Espe, Katharina M. A1 - Raila, Jens A1 - Henze, Andrea A1 - Blouin, Katja A1 - Schneider, A. A1 - Schmiedeke, D. A1 - Krane, Vera A1 - Schweigert, Florian J. A1 - Hocher, Berthold A1 - Wanner, Christoph A1 - Drechsler, Christiane T1 - Low vitamin E plasma levels are associated with cerebrovascular events and mortality in hemodialysis patients T2 - Annals of nutrition & metabolism : journal of nutrition, metabolic diseases and dietetics ; an official journal of International Union of Nutritional Sciences (IUNS) Y1 - 2012 SN - 0250-6807 VL - 60 IS - 2 SP - 137 EP - 137 PB - Karger CY - Basel ER - TY - JOUR A1 - Chupeerach, Chaowanee A1 - Tungtrongchitr, Anchalee A1 - Phonrat, Benjaluck A1 - Schweigert, Florian J. A1 - Tungtrongchitr, Rungsunn A1 - Preutthipan, Sangchai T1 - Association of Thr420Lys polymorphism in DBP gene with fat-soluble vitamins and low radial bone mineral density in postmenopausal Thai women JF - Biomarkers in medicine N2 - Aims: To investigate the genetic markers for osteoporosis bone mineral density by the genotyping of rs7041, rs4588 and rs1352845 in the DBP gene with either bone mineral density or serum 25-hydroxycholecalciferol, retinol and alpha-tocopherol, among 365 postmenopausal Thai women. Materials & methods: The DBP genotypes were analyzed by a PCR restriction fragment-length polymorphism method. Serum 25-hydroxycholecalciferol was assessed using a commercial chemiluminescent immunoassay. Serum retinol and alpha-tocopherol were measured by reverse-phase high-performance liquid chromatography. Results: After adjustment for age >50 years, elder Thai subjects with low BMI (<= 25 kg/m(2)) and carrying the rs4588 CC genotype had a higher risk of radial bone mineral density osteoporosis (odds ratio: 6.29; p = 0.048). The rs1352845 genotype also had a statistical association with total hip bone mineral density; however, it disappeared after adjustment for age and BMI. No association was found in fat-soluble vitamins with bone mineral density. Conclusion: DBP genotypes may influence the osteoporosis bone mineral density in postmenopausal Thai women. KW - bone mineral density KW - fat-soluble vitamin KW - osteoporosis KW - single nucleotide polymorphism KW - vitamin D binding protein Y1 - 2012 U6 - https://doi.org/10.2217/BMM.11.88 SN - 1752-0363 VL - 6 IS - 1 SP - 103 EP - 108 PB - Future Medicine CY - London ER - TY - JOUR A1 - Raila, Jens A1 - Enjalbert, Francis A1 - Mothes, Ralf A1 - Hurtienne, Andrea A1 - Schweigert, Florian J. T1 - Validation of a new point-of-care assay for determination of ss-carotene concentration in bovine whole blood and plasma JF - Veterinary clinical pathology N2 - Background: beta-Carotene is an important precursor of vitamin A, and is associated with bovine fertility. beta-Carotene concentrations in plasma are used to optimize beta-carotene supplementation in cattle, but measurement requires specialized equipment to separate plasma and extract and measure beta-carotene, either using spectrophotometry or high performance liquid chromatography (HPLC). Objective: The objective of this study was to validate a new 2-step point-of-care (POC) assay for measuring beta-carotene in whole blood and plasma. Methods: beta-carotene concentrations in plasma from 166 cows were measured using HPLC and compared with results obtained using a POC assay, the iCheck-iEx-Carotene test kit. Whole blood samples from 23 of these cattle were also evaluated using the POC assay and compared with HPLC-plasma results from the same 23 animals. The POC assay includes an extraction vial (iEx Carotene) and hand-held photometer (iCheck Carotene). Results: Concentrations of beta-carotene in plasma measured using the POC assay ranged from 0.40 to 15.84 mg/L (n = 166). No differences were observed between methods for assay of plasma (mean +/- SD; n = 166): HPLC-plasma 4.23 +/- 2.35 mg/L; POC-plasma 4.49 +/- 2.36 mg/L. Similar good agreement was found when plasma analyzed using HPLC was compared with whole blood analyzed using the POC system (n = 23): HPLC-plasma 3.46 +/- 2.12 mg/L; POC-whole blood 3.67 +/- 2.29 mg/L. Conclusions: Concentrations of beta-carotene can be measured in blood and plasma from cattle easily and rapidly using a POC assay, and results are comparable to those obtained by the highly sophisticated HPLC method. Immediate feedback regarding beta-carotene deficiency facilitates rapid and appropriate optimization of beta-carotene supplementation in feed. KW - Biomarker KW - HPLC KW - method comparison KW - nutritional supplements KW - vitamin A Y1 - 2012 U6 - https://doi.org/10.1111/j.1939-165X.2012.00400.x SN - 0275-6382 VL - 41 IS - 1 SP - 119 EP - 122 PB - Wiley-Blackwell CY - Malden ER - TY - JOUR A1 - Kuhl, Juliane A1 - Aurich, Jörg E. A1 - Wulf, Manuela A1 - Hurtienne, A. A1 - Schweigert, Florian J. A1 - Aurich, Christine T1 - Effects of oral supplementation with beta-carotene on concentrations of beta-carotene, vitamin A and alpha-tocopherol in plasma, colostrum and milk of mares and plasma of their foals and on fertility in mares JF - Journal of animal physiology and animal nutrition N2 - In this study, effects of oral beta-carotene supplementation to mares (beta-carotene group: 1000 mg/day, n = 15; control group: n = 15) from 2 weeks before foaling until 6 weeks thereafter on concentrations of beta-carotene, vitamin A and a-tocopherol in plasma, colostrum and milk and plasma of their foals were determined. In addition, effects on fertility were studied. Beta-carotene concentrations increased in plasma and colostrum of beta-carotene-supplemented mares compared to control mares (p < 0.05). In mares of both groups, beta-carotene concentrations were higher in colostrum than in milk (p < 0.05). In foals, beta-carotene concentrations increased with colostrum uptake and were higher in foals born to supplemented mares (p < 0.05; control group: 0.0003 +/- 0.0002 mu g/ml on day 0, 0.008 +/- 0.0023 mu g/ml on day 1; beta-carotene group: 0.0005 +/- 0.0003 mu g/ml on day 0, 0.048 +/- 0.018 mu g/ml on day 1). Concentrations of vitamin A and a-tocopherol were higher in colostrum than in milk (p < 0.05) but did not differ between groups. Concentration of a-tocopherol in plasma of mares decreased over time and in foals, increased markedly within 4 days after birth. All but one mare (control group) showed oestrus within 2 weeks post-partum. Occurrence of oestrus did not differ between groups. More mares of the control group (7/7 vs. 5/12 in the beta-carotene group) became pregnant after being bred in first post-partum oestrus (p < 0.05). In conclusion, beta-carotene supplementation to mares increased beta-carotene concentrations in plasma, colostrum and milk of mares and plasma of their foals but had no positive effects on fertility. KW - carotene KW - colostrum KW - foal KW - mare KW - plasma KW - a-tocopherol KW - vitamin A Y1 - 2012 U6 - https://doi.org/10.1111/j.1439-0396.2011.01150.x SN - 0931-2439 VL - 96 IS - 3 SP - 376 EP - 384 PB - Wiley-Blackwell CY - Malden ER - TY - JOUR A1 - Bechir, Mahamat A1 - Schelling, E. A1 - Krämer, K. A1 - Schweigert, Florian J. A1 - Bonfoh, Bassirou A1 - Crump, L. A1 - Tanner, M. A1 - Zinsstag, J. T1 - Retinol assessment among women and children in sahelian mobile pastoralists JF - EcoHealth : conservation medicine, human health, ecosystem sustainability N2 - Micronutrient deficiencies are widespread in developing countries, particularly in remote communities such as mobile pastoralists. The nutritional and vitamin A status of this population is not well-documented in Chad. This study assessed serum retinol levels among women and children under five-year-old in nomadic and semi-nomadic pastoralist and rural-settled communities, who are similarly exposed to risk factors such as gastrointestinal parasitic infection, anaemia and emaciation. The novel method of portable fluorometry was used for the first time to measure beta-carotene and retinol levels in a pastoral nomadic area. Moderate level blood retinol deficiency (< 0.7 mu mol/L) was observed in 5% (CI 1-11) of nomadic, 29% (CI 13-45) of semi-nomadic and 22% (CI 8-35) of sedentary women. In children, 1% (CI 0.1-4), 17% (CI 9-25) and 28% (CI 18-39), respectively, had moderate level blood retinol deficiency. In nomadic communities, women and children had blood retinol levels close to normal. Deficiency of retinol was strongly linked with lifestyle (nomadic, semi-nomadic and settled) among women and lifestyle and age among children. The results support an ecological linkage between human retinol levels and livestock milk retinol. This study shows the feasibility of portable retinol and beta-carotene measurement in human blood as well as human and animal milk under remote field conditions, but the approach requires further validation. KW - vitamin A KW - retinol KW - nomadic pastoralist KW - Chad Y1 - 2012 U6 - https://doi.org/10.1007/s10393-012-0781-7 SN - 1612-9202 VL - 9 IS - 2 SP - 113 EP - 121 PB - Springer CY - New York ER - TY - JOUR A1 - Khalil, Mahmoud A1 - Raila, Jens A1 - Ali, Mostafa A1 - Islam, Khan M. S. A1 - Schenk, Regina A1 - Krause, Jens-Peter A1 - Schweigert, Florian J. A1 - Rawel, Harshadrai Manilal T1 - Stability and bioavailability of lutein ester supplements from Tagetes flower prepared under food processing conditions JF - Journal of functional food N2 - Tagetes spp. belongs to the Asteraceae family. It is recognized as a major source of lutein ester (lutein esterified with fatty acids such as lauric, myristic and palmitic acids), a natural colorant belonging to the xanthophylls or oxygenated carotenoids. Four species of Tagetes flower (Tagetes tenuifolia, Tagetes erecta, Tagetes patula, and Tagetes lucida) were used to extract lutein and lutein esters with three different methods. The results showed that T. erecta, type "orangeprinz", is the richest source of lutein esters (14.4 +/- 0.234 mg/g) in comparison to other Tagetes spp. No significant differences between extractions of lutein esters with medium-chain triacylglycerols (MCT) oil, orange oil or solvent (hexane/isopropanol) could be observed. MCT oil also improved stability of lutein esters at 100 degrees C for 40 min. Emulsification of MCT oil improved the stability of lutein ester extract against UV light at 365 nm for 72 h. Finally, an emulsion was prepared under food processing conditions, spray dried and its bioavailability investigated in a preliminary human intervention study. The results show a lower resorption, but further data suggest improvements in implementation of such supplements. (c) 2012 Elsevier Ltd. All rights reserved. KW - Tagetes KW - Lutein ester KW - Emulsion KW - Stability KW - Whey protein KW - Bioavailability Y1 - 2012 U6 - https://doi.org/10.1016/j.jff.2012.03.006 SN - 1756-4646 VL - 4 IS - 3 SP - 602 EP - 610 PB - Elsevier CY - Amsterdam ER - TY - CHAP A1 - Doyon, Anke A1 - Schmiedchen, Bettina A1 - Bayazit, Aysun A1 - Canpolat, Nur A1 - Duzova, Ali A1 - Kracht, Daniela A1 - Litwin, Mieczyslaw A1 - Niemirska, Anna A1 - Sozeri, Betul A1 - Zeller, Rene A1 - Ranchin, Bruno A1 - Anarat, Ali A1 - Caliskan, Salim A1 - Mir, Sevgi A1 - Melk, Anette A1 - Wühl, Elke A1 - Schweigert, Florian J. A1 - Querfeld, Uwe A1 - Schäfer, Franz T1 - Distribuion and determinants of serum vitamin d concentrations in european children with chronic kidney disease T2 - Pediatric nephrology : journal of the International Pediatric Nephrology Association Y1 - 2012 SN - 0931-041X VL - 27 IS - 9 SP - 1627 EP - 1628 PB - Springer CY - New York ER - TY - CHAP A1 - Doyon, Anke A1 - Schmiedchen, Bettina A1 - Bayazit, Aysun A1 - Canpolat, Nur A1 - Duzova, Ali A1 - Kracht, Daniela A1 - Litwin, Mieczyslaw A1 - Niemirska, Anna A1 - Sozeri, Betul A1 - Zeller, Rene A1 - Anarat, Ali A1 - Caliskan, Salim A1 - Mir, Sevgi A1 - Shroff, Rukshana A1 - Melk, Anette A1 - Wühl, Elke A1 - Schweigert, Florian J. A1 - Querfeld, Uwe A1 - Schäfer, Franz T1 - Altered arterial morphology and function in children with CKD Role of mineral-bone disorder T2 - Pediatric nephrology : journal of the International Pediatric Nephrology Association Y1 - 2012 SN - 0931-041X VL - 27 IS - 9 SP - 1606 EP - 1607 PB - Springer CY - New York ER - TY - JOUR A1 - Elias-Miro, Maria A1 - Massip-Salcedo, Marta A1 - Raila, Jens A1 - Schweigert, Florian J. A1 - Mendes-Braz, Mariana A1 - Ramalho, Fernando A1 - Jimenez-Castro, Monica B. A1 - Casillas-Ramirez, Arani A1 - Bermudo, Raquel A1 - Rimola, Antoni A1 - Rodes, Juan A1 - Peralta, Carmen T1 - Retinol binding protein 4 and retinol in steatotic and nonsteatotic rat livers in the setting of partial hepatectomy under ischemia/reperfusion JF - Liver transplantation N2 - Steatotic livers show increased hepatic damage and impaired regeneration after partial hepatectomy (PH) under ischemia/reperfusion (I/R), which is commonly applied in clinical practice to reduce bleeding. The known function of retinol-binding protein 4 (RBP4) is to transport retinol in the circulation. We examined whether modulating RBP4 and/or retinol could protect steatotic and nonsteatotic livers in the setting of PH under I/R. Steatotic and nonsteatotic livers from Zucker rats were subjected to PH (70%) with 60 minutes of ischemia. RBP4 and retinol levels were measured and altered pharmacologically, and their effects on hepatic damage and regeneration were studied after reperfusion. Decreased RBP4 levels were observed in both liver types, whereas retinol levels were reduced only in steatotic livers. RBP4 administration exacerbated the negative consequences of liver surgery with respect to damage and liver regeneration in both liver types. RBP4 affected the mobilization of retinol from steatotic livers, and this revealed actions of RBP4 independent of simple retinol transport. The injurious effects of RBP4 were not due to changes in retinol levels. Treatment with retinol was effective only for steatotic livers. Indeed, retinol increased hepatic injury and impaired liver regeneration in nonsteatotic livers. In steatotic livers, retinol reduced damage and improved regeneration after surgery. These benefits of retinol were associated with a reduced accumulation of hepatocellular fat. Thus, strategies based on modulating RBP4 could be ineffective and possibly even harmful in both liver types in the setting of PH under I/R. In terms of clinical applications, a retinol pretreatment might open new avenues for liver surgery that specifically benefit the steatotic liver. Liver Transpl 18:1198-1208, 2012. Y1 - 2012 U6 - https://doi.org/10.1002/lt.23489 SN - 1527-6465 VL - 18 IS - 10 SP - 1198 EP - 1208 PB - Wiley-Blackwell CY - Hoboken ER -