TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Bauer, Christian G.
A1  - Makower, Alexander
A1  - Wollenberger, Ursula
A1  - Warsinke, Axel
A1  - Bier, Frank Fabian
T1  - Immunoassays using enzymatic amplification electrodes
Y1  - 2002
SN  - 0-7484-0791-X
ER  - 
TY  - BOOK
A1  - Wollenberger, Ursula
A1  - Renneberg, Reinhard
A1  - Bier, Frank Fabian
A1  - Scheller, Frieder W.
T1  - Analytische Biochemie : eine praktische Einführung in das Messen mit Biomolekülen
Y1  - 2003
SN  - 3-527-30166-6
PB  - John Wiley & Sons
CY  - Hoboken
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Bier, Frank Fabian
T1  - Biosensoren
Y1  - 2003
ER  - 
TY  - JOUR
A1  - Ehrentreich-Förster, Eva
A1  - Scheller, Frieder W.
A1  - Bier, Frank Fabian
T1  - Detection of progesterone in whole blood samples
N2  - The progesterone concentration in blood samples can be utilised as a marker for the diagnosis of early pregnancy, endocrinopathy and virilism. Here, we describe a method for progesterone detection and measurement in whole blood samples by a surface sensitive biosensor used in conjunction with an integrated optical grating coupler. This device determines refractive index changes near the biosensor's surface. Hence, biological species bound to a surface layer can be measured in real-time without any label. For the measurements, we have modified the indirect competitive immonoassay principle. The concentration of the progesterone antibody was kept at 1 µg/ml. Progesterone concentration was determined in buffer solution and whole blood in a range between 0.005 and 10 ng/ml. The detection limit was determined to be 3 pM. The relative standard deviation was calculated to be 3.5%.
Y1  - 2003
ER  - 
TY  - JOUR
A1  - Scheller, Frieder W.
A1  - Bier, Frank Fabian
T1  - Analytical Biochemistry (Editorial)
Y1  - 2004
ER  - 
TY  - JOUR
A1  - Steffen, Jenny
A1  - von Nickisch-Rosenegk, Markus
A1  - Bier, Frank Fabian
T1  - In vitro transcription of a whole gene on a surface-coupled template
N2  - An artificial gene was constructed combining the T7 promoter and terminator with the EGFP-gene from the plasmid pEGFP. The functionality of the construct was shown by in vitro translation. The gene-construct was immobilised on a planar glass surface. The transcription was performed on the immobilised gene and mRNA was determined by RT-PCR. Multiple use of the immobilised gene was demonstrated
Y1  - 2005
SN  - 1473-0189
ER  - 
TY  - JOUR
A1  - Heise, Christian
A1  - Bier, Frank Fabian
T1  - Immobilization of DNA on microarrays
N2  - Microarrays are new analytical devices that allow the parallel and simultaneous detection of thousands of target compounds. Microarrays, also called DNA chips, are widely used in gene expression, the genotyping of individuals, point mutations, detection of single nucleotide polymorphisms, and short tandem repeats. Microarrays have highly specific base-pair interactions with labeled complementary strands, which makes this technology to a powerful analytical device for monitoring whole genomes. In this article, we provide a survey of the common microarray manufacturing methods, from the selection of support material to surface structuring, immobilization and hybridization, and finally the detection with labeled complementary strands. Special attention is given to the immobilization of single strands, since fast chemical reactions, the creation of homogeneous surface functionalities as well as an oriented coupling are crucial pre-conditions for a good spot morphology and microarrays of high quality
Y1  - 2005
ER  -