TY  - GEN
A1  - Nicolai, Merle Marie
A1  - Weishaupt, Ann-Kathrin
A1  - Baesler, Jessica
A1  - Brinkmann, Vanessa
A1  - Wellenberg, Anna
A1  - Winkelbeiner, Nicola Lisa
A1  - Gremme, Anna
A1  - Aschner, Michael
A1  - Fritz, Gerhard
A1  - Schwerdtle, Tanja
A1  - Bornhorst, Julia
T1  - Effects of manganese on genomic integrity in the multicellular model organism Caenorhabditis elegans
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Although manganese (Mn) is an essential trace element, overexposure is associated with Mn-induced toxicity and neurological dysfunction. Even though Mn-induced oxidative stress is discussed extensively, neither the underlying mechanisms of the potential consequences of Mn-induced oxidative stress on DNA damage and DNA repair, nor the possibly resulting toxicity are characterized yet. In this study, we use the model organism Caenorhabditis elegans to investigate the mode of action of Mn toxicity, focusing on genomic integrity by means of DNA damage and DNA damage response. Experiments were conducted to analyze Mn bioavailability, lethality, and induction of DNA damage. Different deletion mutant strains were then used to investigate the role of base excision repair (BER) and dePARylation (DNA damage response) proteins in Mn-induced toxicity. The results indicate a dose- and time-dependent uptake of Mn, resulting in increased lethality. Excessive exposure to Mn decreases genomic integrity and activates BER. Altogether, this study characterizes the consequences of Mn exposure on genomic integrity and therefore broadens the molecular understanding of pathways underlying Mn-induced toxicity. Additionally, studying the basal poly(ADP-ribosylation) (PARylation) of worms lacking poly(ADP-ribose) glycohydrolase (PARG) parg-1 or parg-2 (two orthologue of PARG), indicates that parg-1 accounts for most of the glycohydrolase activity in worms.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1173 
KW  - manganese
KW  - oxidative stress
KW  - DNA repair
KW  - DNA damage response
KW  - Caenorhabditis elegans
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-523275
SN  - 1866-8372
IS  - 1173
ER  - 
TY  - GEN
A1  - Baesler, Jessica
A1  - Michaelis, Vivien
A1  - Stiboller, Michael
A1  - Haase, Hajo
A1  - Aschner, Michael
A1  - Schwerdtle, Tanja
A1  - Sturzenbaum, Stephen R.
A1  - Bornhorst, Julia
T1  - Nutritive manganese and zinc overdosing in aging c. elegans result in a metallothionein-mediated alteration in metal homeostasis
T2  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Manganese (Mn) and zinc (Zn) are not only essential trace elements, but also potential exogenous risk factors for various diseases. Since the disturbed homeostasis of single metals can result in detrimental health effects, concerns have emerged regarding the consequences of excessive exposures to multiple metals, either via nutritional supplementation or parenteral nutrition. This study focuses on Mn-Zn-interactions in the nematode Caenorhabditis elegans (C. elegans) model, taking into account aspects related to aging and age-dependent neurodegeneration.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1364 
KW  - aging
KW  - C. elegans
KW  - homeostasis
KW  - manganese
KW  - zinc
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-514995
SN  - 1866-8372
IS  - 8
ER  -