TY - JOUR A1 - Kiemel, Katrin A1 - Gurke, Marie A1 - Paraskevopoulou, Sofia A1 - Havenstein, Katja A1 - Weithoff, Guntram A1 - Tiedemann, Ralph T1 - Variation in heat shock protein 40 kDa relates to divergence in thermotolerance among cryptic rotifer species JF - Scientific reports N2 - Genetic divergence and the frequency of hybridization are central for defining species delimitations, especially among cryptic species where morphological differences are merely absent. Rotifers are known for their high cryptic diversity and therefore are ideal model organisms to investigate such patterns. Here, we used the recently resolved Brachionus calyciflorus species complex to investigate whether previously observed between species differences in thermotolerance and gene expression are also reflected in their genomic footprint. We identified a Heat Shock Protein gene (HSP 40 kDa) which exhibits cross species pronounced sequence variation. This gene exhibits species-specific fixed sites, alleles, and sites putatively under positive selection. These sites are located in protein binding regions involved in chaperoning and may therefore reflect adaptive diversification. By comparing three genetic markers (ITS, COI, HSP 40 kDa), we revealed hybridization events between the cryptic species. The low frequency of introgressive haplotypes/alleles suggest a tight, but not fully impermeable boundary between the cryptic species. Y1 - 2022 U6 - https://doi.org/10.1038/s41598-022-27137-3 SN - 2045-2322 VL - 12 IS - 1 PB - Macmillan Publishers Limited CY - London ER - TY - JOUR A1 - Tebbe, Jonas A1 - Ottensmann, Meinolf A1 - Havenstein, Katja A1 - Efstratiou, Artemis A1 - Lenz, Tobias L. A1 - Caspers, Barbara A. A1 - Forcada, Jaume A1 - Tiedemann, Ralph A1 - Hoffman, Joseph T1 - Intronic primers reveal unexpectedly high major histocompatibility complex diversity in Antarctic fur seals JF - Scientific reports N2 - The major histocompatibility complex (MHC) is a group of genes comprising one of the most important components of the vertebrate immune system. Consequently, there has been much interest in characterising MHC variation and its relationship with fitness in a variety of species. Due to the exceptional polymorphism of MHC genes, careful PCR primer design is crucial for capturing all of the allelic variation present in a given species. We therefore developed intronic primers to amplify the full-length 267 bp protein-coding sequence of the MHC class II DQB exon 2 in the Antarctic fur seal. We then characterised patterns of MHC variation among mother-offspring pairs from two breeding colonies and detected 19 alleles among 771 clone sequences from 56 individuals. The distribution of alleles within and among individuals was consistent with a single-copy, classical DQB locus showing Mendelian inheritance. Amino acid similarity at the MHC was significantly associated with genome-wide relatedness, but no relationship was found between MHC heterozygosity and genome-wide heterozygosity. Finally, allelic diversity was several times higher than reported by a previous study based on partial exon sequences. This difference appears to be related to allele-specific amplification bias, implying that primer design can strongly impact the inference of MHC diversity. Y1 - 2022 U6 - https://doi.org/10.1038/s41598-022-21658-7 SN - 2045-2322 VL - 12 IS - 1 PB - Nature Publishing Group CY - London ER - TY - GEN A1 - Kiemel, Katrin A1 - Gurke, Marie A1 - Paraskevopoulou, Sofia A1 - Havenstein, Katja A1 - Weithoff, Guntram A1 - Tiedemann, Ralph T1 - Variation in heat shock protein 40 kDa relates to divergence in thermotolerance among cryptic rotifer species T2 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe N2 - Genetic divergence and the frequency of hybridization are central for defining species delimitations, especially among cryptic species where morphological differences are merely absent. Rotifers are known for their high cryptic diversity and therefore are ideal model organisms to investigate such patterns. Here, we used the recently resolved Brachionus calyciflorus species complex to investigate whether previously observed between species differences in thermotolerance and gene expression are also reflected in their genomic footprint. We identified a Heat Shock Protein gene (HSP 40 kDa) which exhibits cross species pronounced sequence variation. This gene exhibits species-specific fixed sites, alleles, and sites putatively under positive selection. These sites are located in protein binding regions involved in chaperoning and may therefore reflect adaptive diversification. By comparing three genetic markers (ITS, COI, HSP 40 kDa), we revealed hybridization events between the cryptic species. The low frequency of introgressive haplotypes/alleles suggest a tight, but not fully impermeable boundary between the cryptic species. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1305 Y1 - 2023 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-578635 SN - 1866-8372 IS - 1305 ER - TY - JOUR A1 - Kiemel, Katrin A1 - Gurke, Marie A1 - Paraskevopoulou, Sofia A1 - Havenstein, Katja A1 - Weithoff, Guntram A1 - Tiedemann, Ralph T1 - Variation in heat shock protein 40 kDa relates to divergence in thermotolerance among cryptic rotifer species JF - Scientific Reports N2 - Genetic divergence and the frequency of hybridization are central for defining species delimitations, especially among cryptic species where morphological differences are merely absent. Rotifers are known for their high cryptic diversity and therefore are ideal model organisms to investigate such patterns. Here, we used the recently resolved Brachionus calyciflorus species complex to investigate whether previously observed between species differences in thermotolerance and gene expression are also reflected in their genomic footprint. We identified a Heat Shock Protein gene (HSP 40 kDa) which exhibits cross species pronounced sequence variation. This gene exhibits species-specific fixed sites, alleles, and sites putatively under positive selection. These sites are located in protein binding regions involved in chaperoning and may therefore reflect adaptive diversification. By comparing three genetic markers (ITS, COI, HSP 40 kDa), we revealed hybridization events between the cryptic species. The low frequency of introgressive haplotypes/alleles suggest a tight, but not fully impermeable boundary between the cryptic species. Y1 - 2022 U6 - https://doi.org/10.1038/s41598-022-27137-3 SN - 2045-2322 VL - 12 PB - Springer Nature CY - London ER - TY - GEN A1 - Spikes, Montrai A1 - Rodríguez-Silva, Rodet A1 - Bennett, Kerri-Ann A1 - Bräger, Stefan A1 - Josaphat, James A1 - Torres-Pineda, Patricia A1 - Ernst, Anja A1 - Havenstein, Katja A1 - Schlupp, Ingo A1 - Tiedemann, Ralph T1 - A phylogeny of the genus Limia (Teleostei: Poeciliidae) suggests a single-lake radiation nested in a Caribbean-wide allopatric speciation scenario T2 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe N2 - Objective The Caribbean is an important global biodiversity hotspot. Adaptive radiations there lead to many speciation events within a limited period and hence are particularly prominent biodiversity generators. A prime example are freshwater fish of the genus Limia, endemic to the Greater Antilles. Within Hispaniola, nine species have been described from a single isolated site, Lake Miragoâne, pointing towards extraordinary sympatric speciation. This study examines the evolutionary history of the Limia species in Lake Miragoâne, relative to their congeners throughout the Caribbean. Results For 12 Limia species, we obtained almost complete sequences of the mitochondrial cytochrome b gene, a well-established marker for lower-level taxonomic relationships. We included sequences of six further Limia species from GenBank (total N  = 18 species). Our phylogenies are in concordance with other published phylogenies of Limia. There is strong support that the species found in Lake Miragoâne in Haiti are monophyletic, confirming a recent local radiation. Within Lake Miragoâne, speciation is likely extremely recent, leading to incomplete lineage sorting in the mtDNA. Future studies using multiple unlinked genetic markers are needed to disentangle the relationships within the Lake Miragoâne clade. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1238 KW - Cytochrome b KW - Island biogeography KW - Fresh water fish KW - Phylogeny Y1 - 2022 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-548882 SN - 1866-8372 SP - 1 EP - 8 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - JOUR A1 - Spikes, Montrai A1 - Rodríguez-Silva, Rodet A1 - Bennett, Kerri-Ann A1 - Bräger, Stefan A1 - Josaphat, James A1 - Torres-Pineda, Patricia A1 - Ernst, Anja A1 - Havenstein, Katja A1 - Schlupp, Ingo A1 - Tiedemann, Ralph T1 - A phylogeny of the genus Limia (Teleostei: Poeciliidae) suggests a single-lake radiation nested in a Caribbean-wide allopatric speciation scenario JF - BMC Research Notes N2 - Objective The Caribbean is an important global biodiversity hotspot. Adaptive radiations there lead to many speciation events within a limited period and hence are particularly prominent biodiversity generators. A prime example are freshwater fish of the genus Limia, endemic to the Greater Antilles. Within Hispaniola, nine species have been described from a single isolated site, Lake Miragoâne, pointing towards extraordinary sympatric speciation. This study examines the evolutionary history of the Limia species in Lake Miragoâne, relative to their congeners throughout the Caribbean. Results For 12 Limia species, we obtained almost complete sequences of the mitochondrial cytochrome b gene, a well-established marker for lower-level taxonomic relationships. We included sequences of six further Limia species from GenBank (total N  = 18 species). Our phylogenies are in concordance with other published phylogenies of Limia. There is strong support that the species found in Lake Miragoâne in Haiti are monophyletic, confirming a recent local radiation. Within Lake Miragoâne, speciation is likely extremely recent, leading to incomplete lineage sorting in the mtDNA. Future studies using multiple unlinked genetic markers are needed to disentangle the relationships within the Lake Miragoâne clade. KW - Cytochrome b KW - Island biogeography KW - Fresh water fish KW - Phylogeny Y1 - 2021 U6 - https://doi.org/10.1186/s13104-021-05843-x SN - 1756-0500 VL - 14 SP - 1 EP - 8 PB - BMC Research Notes / Biomed Central CY - London ER - TY - JOUR A1 - Senczuk, Gabriele A1 - Havenstein, Katja A1 - Milana, Valentina A1 - Ripa, Chiara A1 - De Simone, Emanuela A1 - Tiedemann, Ralph A1 - Castiglia, Riccardo T1 - Spotlight on islands BT - on the origin and diversification of an ancient lineage of the Italian wall lizard Podarcis siculus in the western Pontine Islands JF - Scientific reports N2 - Groups of proximate continental islands may conceal more tangled phylogeographic patterns than oceanic archipelagos as a consequence of repeated sea level changes, which allow populations to experience gene flow during periods of low sea level stands and isolation by vicariant mechanisms during periods of high sea level stands. Here, we describe for the first time an ancient and diverging lineage of the Italian wall lizard Podarcis siculus from the western Pontine Islands. We used nuclear and mitochondrial DNA sequences of 156 individuals with the aim of unraveling their phylogenetic position, while microsatellite loci were used to test several a priori insular biogeographic models of migration with empirical data. Our results suggest that the western Pontine populations colonized the islands early during their Pliocene volcanic formation, while populations from the eastern Pontine Islands seem to have been introduced recently. The inter-island genetic makeup indicates an important role of historical migration, probably due to glacial land bridges connecting islands followed by a recent vicariant mechanism of isolation. Moreover, the most supported migration model predicted higher gene flow among islands which are geographically arranged in parallel. Considering the threatened status of small insular endemic populations, we suggest this new evolutionarily independent unit be given priority in conservation efforts. Y1 - 2018 U6 - https://doi.org/10.1038/s41598-018-33326-w SN - 2045-2322 VL - 8 PB - Nature Publ. Group CY - London ER - TY - GEN A1 - Senczuk, Gabriele A1 - Havenstein, Katja A1 - Milana, Valentina A1 - Ripa, Chiara A1 - De Simone, Emanuela A1 - Tiedemann, Ralph A1 - Castiglia, Riccardo T1 - Spotlight on islands BT - on the origin and diversification of an ancient lineage of the Italian wall lizard Podarcis siculus in the western Pontine Islands T2 - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe N2 - Groups of proximate continental islands may conceal more tangled phylogeographic patterns than oceanic archipelagos as a consequence of repeated sea level changes, which allow populations to experience gene flow during periods of low sea level stands and isolation by vicariant mechanisms during periods of high sea level stands. Here, we describe for the first time an ancient and diverging lineage of the Italian wall lizard Podarcis siculus from the western Pontine Islands. We used nuclear and mitochondrial DNA sequences of 156 individuals with the aim of unraveling their phylogenetic position, while microsatellite loci were used to test several a priori insular biogeographic models of migration with empirical data. Our results suggest that the western Pontine populations colonized the islands early during their Pliocene volcanic formation, while populations from the eastern Pontine Islands seem to have been introduced recently. The inter-island genetic makeup indicates an important role of historical migration, probably due to glacial land bridges connecting islands followed by a recent vicariant mechanism of isolation. Moreover, the most supported migration model predicted higher gene flow among islands which are geographically arranged in parallel. Considering the threatened status of small insular endemic populations, we suggest this new evolutionarily independent unit be given priority in conservation efforts. T3 - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 991 KW - mitochondrial dna sequences KW - genetic diversity KW - haplotype reconstruction KW - evolutionary history KW - geological evolution KW - population structure KW - Tyrrhenian Sea KW - endemic lizard KW - genotype data KW - biogeography Y1 - 2020 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-446360 SN - 1866-8372 IS - 991 ER - TY - JOUR A1 - Valente, Luis A1 - Illera, Juan Carlos A1 - Havenstein, Katja A1 - Pallien, Tamara A1 - Etienne, Rampal S. A1 - Tiedemann, Ralph T1 - Equilibrium Bird Species Diversity in Atlantic Islands JF - Current biology Y1 - 2017 U6 - https://doi.org/10.1016/j.cub.2017.04.053 SN - 0960-9822 SN - 1879-0445 VL - 27 SP - 1660 EP - + PB - Cell Press CY - Cambridge ER - TY - JOUR A1 - Marrone, F. A1 - Havenstein, Katja A1 - Tiedemann, Ralph A1 - Ketmaier, V. T1 - Identification and characterization of five polymorphic microsatellite loci in the freshwater copepod Hemidiaptomus gurneyi (Copepoda: Calanoida: Diaptomidae) JF - The Italian journal of zoology N2 - Hemidiaptomus diaptomid copepods are known to be excellent biological indicators for the highly biodiverse crustacean communities inhabiting Mediterranean temporary ponds (MTPs), an endangered inland water habitat whose conservation is considered a priority according to the "Habitat Directive" of the European Union. This study reports on the characterization of five polymorphic microsatellite loci in Hemidiaptomus gurneyi, to be used as markers for fine-scale studies on the population genetic structure and metapopulation dynamics of a typical and obligate MTP dweller. The five selected loci proved to be polymorphic in the species, with three to five polymorphic loci per studied population. Overall, mean heterozygosity scored for all loci and populations was lower than that reported for the few other diaptomid species for which microsatellite loci have been to date described; this is possibly due to the intrinsically fragmented and isolated peculiar habitat inhabited by the species. Furthermore, the presence of indels within the flanking regions of selected loci was scored. This study, albeit confirming the technical difficulties in finding proper microsatellite markers in copepods, provides for the first time a set of useful polymorphic microsatellite loci for a Hemidiaptomus species, thus allowing the realization of fine-scale phylogeographic and population genetics studies of this flagship crustacean taxon for MTPs. KW - Mediterranean temporary ponds KW - diaptomid copepods KW - SSRs Y1 - 2016 U6 - https://doi.org/10.1080/11250003.2015.1126363 SN - 1125-0003 SN - 1748-5851 VL - 83 SP - 146 EP - 150 PB - Springer CY - Abingdon ER -