TY  - GEN
A1  - Liu, Qingting
A1  - Li, Xiaoping
A1  - Fettke, Jörg
T1  - Starch granules in Arabidopsis thaliana mesophyll and guard cells show similar morphology but differences in size and number
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Transitory starch granules result from complex carbon turnover and display specific situations during starch synthesis and degradation. The fundamental mechanisms that specify starch granule characteristics, such as granule size, morphology, and the number per chloroplast, are largely unknown. However, transitory starch is found in the various cells of the leaves of Arabidopsis thaliana, but comparative analyses are lacking. Here, we adopted a fast method of laser confocal scanning microscopy to analyze the starch granules in a series of Arabidopsis mutants with altered starch metabolism. This allowed us to separately analyze the starch particles in the mesophyll and in guard cells. In all mutants, the guard cells were always found to contain more but smaller plastidial starch granules than mesophyll cells. The morphological properties of the starch granules, however, were indiscernible or identical in both types of leaf cells.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1143 
KW  - starch granules
KW  - starch metabolism
KW  - starch granule initiation
KW  - starch granule number per chloroplast
KW  - starch morphology
KW  - mesophyll cell
KW  - guard cell
KW  - LCSM
KW  - Arabidopsis thaliana
Y1  - 2021
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-511067
SN  - 1866-8372
IS  - 1143
ER  - 
TY  - GEN
A1  - Liu, Qingting
A1  - Zhou, Yuan
A1  - Fettke, Jörg
T1  - Starch granule size and morphology of Arabidopsis thaliana starch-related mutants analyzed during diurnal rhythm and development
T2  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Transitory starch plays a central role in the life cycle of plants. Many aspects of this important metabolism remain unknown; however, starch granules provide insight into this persistent metabolic process. Therefore, monitoring alterations in starch granules with high temporal resolution provides one significant avenue to improve understanding. Here, a previously established method that combines LCSM and safranin-O staining for in vivo imaging of transitory starch granules in leaves of Arabidopsis thaliana was employed to demonstrate, for the first time, the alterations in starch granule size and morphology that occur both throughout the day and during leaf aging. Several starch-related mutants were included, which revealed differences among the generated granules. In ptst2 and sex1-8, the starch granules in old leaves were much larger than those in young leaves; however, the typical flattened discoid morphology was maintained. In ss4 and dpe2/phs1/ss4, the morphology of starch granules in young leaves was altered, with a more rounded shape observed. With leaf development, the starch granules became spherical exclusively in dpe2/phs1/ss4. Thus, the presented data provide new insights to contribute to the understanding of starch granule morphogenesis.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1245 
KW  - starch metabolism
KW  - starch granule
KW  - starch granule size
KW  - starch granule morphology
KW  - LCSM
KW  - Arabidopsis thaliana
Y1  - 2022
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-550291
SN  - 1866-8372
VL  - 26
SP  - 1
EP  - 9
PB  - Universitätsverlag Potsdam
CY  - Potsdam
ET  - 19
ER  -