TY - JOUR A1 - Kurbanoglu, Sevinc A1 - Yarman, Aysu T1 - Simultaneous determination of hydrochlorothiazide and irbesartan from pharmaceutical dosage forms with RP-HPLC T1 - Farmasötik Dozaj Formlarında TF-YPSK ile Hidroklorotiyazid ve İrbesartanın Eş Zamanlı Tayini JF - Turkish journal of pharmaceutical sciences N2 - Objectives: In this work, a simple and rapid liquid chromatographic method for the simultaneous determination of irbesartan (IRBE) and hydrochlorothiazide (HCT) was developed and validated by reverse phase high performance liquid chromatography (RP-HPLC).
Materials and Methods: Experimental conditions such as different buffer solutions, various pH values, temperature, composition of the mobile phase, and the effect of flow rate were optimized.
Results: The developed RP-HPLC method for these antihypertensive agents was wholly validated and IRBE was detected in the linear range of 0.1-25 mu g mL(-1) and HCT was detected in the linear range of 0.25-25 mu g mL(-1). Moreover, the suggested chromatographic technique was successfully applied for the determination of the drugs in human serum and pharmaceutical dosage forms with limit of detection values of 0.008 mu g mL(-1) for IRBE and 0.012 mu g mL(-1) for HCT.
Conclusion: The proposed rapid analysis method of these antihypertensive drugs can be easily used and applied by pharmaceutical companies for which the analysis time is important. N2 - Amaç: Bu çalışmada, irbesartan (IRBE) ve hidroklorotiyazidin (HCT) eşzamanlı tayini için basit ve hızlı bir ters fazlı yüksek performanslı sıvı kromatografisi (TF-YPSK) yöntemi geliştirilmiş ve validasyon çalışmaları yapılmıştır. Gereç ve Yöntemler: Deneysel koşullar; farklı tampon çözeltileri, çeşitli pH değerleri, sıcaklık, mobil fazın bileşimi, akış hızının etkisi gibi parametrelerin üzerinden optimize edildi. Bulgular: Bu antihipertansif ajanlar için geliştirilen TF-YPSK yönteminin tüm validasyon parametrelerine ilişkin çalışmalar yapılmış, ve IRBE 0,1-25 μg mL-1 doğrusal aralığında ve HCT 0,25-25 μg mL-1 doğrusal aralığında tespit edilmiştir. Ayrıca önerilen TF-YPSK yöntemi ile IRBE için 0,008 μg mL-1 ve HCT için 0,012 μg mL-1 tayin alt sınır değerleri bulunmuştur. Geliştirilen yöntem, insan serumunda ve farmasötik dozaj formlarında bulunan IRBE ve HCT’nin belirlenmesi için başarıyla uygulanmıştır. Sonuç: Bu antihipertansif ilaçların miktar tayininde önerilen YPSK analiz yönteminin, analiz süresinin önemli olduğu ilaç firmalarında rahatlıkla kullanılabileceği ve uygulanabileceği düşünülmektedir. KW - HPLC KW - irbesartan KW - hydrochlorothiazide KW - pharmaceutical dosage forms Y1 - 2020 U6 - https://doi.org/10.4274/tjps.galenos.2019.76094 SN - 1304-530X VL - 17 IS - 5 SP - 523 EP - 527 PB - Turkish Pharmacists Association CY - Çankaya-Ankara ER - TY - JOUR A1 - Watanabe, Mutsumi A1 - Tohge, Takayuki A1 - Balazadeh, Salma A1 - Erban, Alexander A1 - Giavalisco, Patrick A1 - Kopka, Joachim A1 - Mueller-Roeber, Bernd A1 - Fernie, Alisdair R. A1 - Hoefgen, Rainer T1 - Comprehensive Metabolomics Studies of Plant Developmental Senescence JF - Plant Senescence: Methods and Protocols N2 - Leaf senescence is an essential developmental process that involves diverse metabolic changes associated with degradation of macromolecules allowing nutrient recycling and remobilization. In contrast to the significant progress in transcriptomic analysis of leaf senescence, metabolomics analyses have been relatively limited. A broad overview of metabolic changes during leaf senescence including the interactions between various metabolic pathways is required to gain a better understanding of the leaf senescence allowing to link transcriptomics with metabolomics and physiology. In this chapter, we describe how to obtain comprehensive metabolite profiles and how to dissect metabolic shifts during leaf senescence in the model plant Arabidopsis thaliana. Unlike nucleic acid analysis for transcriptomics, a comprehensive metabolite profile can only be achieved by combining a suite of analytic tools. Here, information is provided for measurements of the contents of chlorophyll, soluble proteins, and starch by spectrophotometric methods, ions by ion chromatography, thiols and amino acids by HPLC, primary metabolites by GC/TOF-MS, and secondary metabolites and lipophilic metabolites by LC/ESI-MS. These metabolite profiles provide a rich catalogue of metabolic changes during leaf senescence, which is a helpful database and blueprint to be correlated to future studies such as transcriptome and proteome analyses, forward and reverse genetic studies, or stress-induced senescence studies. KW - Senescence KW - Metabolomics KW - Arabidopsis KW - GC/MS KW - LC/MS KW - HPLC KW - IC Y1 - 2018 SN - 978-1-4939-7672-0 SN - 978-1-4939-7670-6 U6 - https://doi.org/10.1007/978-1-4939-7672-0_28 SN - 1064-3745 SN - 1940-6029 VL - 1744 SP - 339 EP - 358 PB - Humana Press CY - Totowa ER -