TY - GEN A1 - Radosavljevic, Boris A1 - Lantuit, Hugues A1 - Pollard, Wayne A1 - Overduin, Pier Paul A1 - Couture, Nicole A1 - Sachs, Torsten A1 - Helm, Veit A1 - Fritz, Michael T1 - Erosion and Flooding - Threats to Coastal Infrastructure in the Arctic: A Case Study from Herschel Island, Yukon Territory, Canada (vol 39, pg 900, 2016) T2 - Estuaries and coasts : journal of the Estuarine Research Federation Y1 - 2016 U6 - https://doi.org/10.1007/s12237-016-0115-z SN - 1559-2723 SN - 1559-2731 VL - 39 SP - 1294 EP - 1295 PB - Springer CY - New York ER - TY - GEN A1 - Cajar, Anke A1 - Engbert, Ralf A1 - Laubrock, Jochen T1 - Eye movements during gaze-contingent spatial-frequency filtering of real-world scenes: Effects of filter location, cutoff, and size T2 - Perception Y1 - 2016 SN - 0301-0066 SN - 1468-4233 VL - 45 SP - 126 EP - 126 PB - Sage Publ. CY - London ER - TY - GEN A1 - Kruegel, Andre A1 - Engbert, Ralf T1 - Statistics of microsaccades indicate early frequency effects during visual word recognition T2 - Perception Y1 - 2016 SN - 0301-0066 SN - 1468-4233 VL - 45 SP - 127 EP - 127 PB - Sage Publ. CY - London ER - TY - GEN A1 - Hanack, Katja A1 - Schloer, Anja A1 - Holzloehner, Pamela A1 - Listek, Martin A1 - Bauer, Cindy A1 - Butze, Monique A1 - Micheel, Burkhard A1 - Hentschel, Christian A1 - Sowa, Mandy A1 - Roggenbuck, Dirk A1 - Schierack, Peter A1 - Fuener, Jonas A1 - Schliebs, Erik A1 - Goihl, Alexander A1 - Reinhold, Dirk T1 - Camelid nanobodies specific to human pancreatic glycoprotein 2 T2 - The journal of immunology N2 - Pancreatic secretory zymogen-granule membrane glycoprotein 2 (GP2) has been identified to be a major autoantigenic target in Crohn’s disease patients. It was discussed recently that a long and a short isoform of GP2 exists whereas the short isoform is often detected by GP2-specific autoantibodies. In the outcome of inflammatory bowel diseases, these GP2-specific autoantibodies are discussed as new serological markers for diagnosis and therapeutic monitoring. To investigate this further, camelid nanobodies were generated by phage display and selected against the short isoform of GP2 in order to isolate specific tools for the discrimination of both isoforms. Nanobodies are single domain antibodies derived from camelid heavy chain only antibodies and characterized by a high stability and solubility. The selected candidates were expressed, purified and validated regarding their binding properties in different enzyme-linked immunosorbent assays formats, immunofluorescence, immunohistochemistry and surface plasmon resonance spectroscopy. Four different nanobodies could be selected whereof three recognize the short isoform of GP2 very specifically and one nanobody showed a high binding capacity for both isoforms. The KD values measured for all nanobodies were between 1.3 nM and 2.3 pM indicating highly specific binders suitable for the application as diagnostic tool in inflammatory bowel disease. Y1 - 2016 SN - 0022-1767 SN - 1550-6606 VL - 196 SP - 313 EP - 328 PB - American Assoc. of Immunologists CY - Bethesda ER - TY - GEN A1 - Maier, Natalia A1 - Holzlöhner, Pamela A1 - Hoenow, Anja A1 - Scheunemann, Astrid A1 - Weschke, Daniel A1 - Hanack, Katja T1 - Characterization of monoclonal antibodies generated by in vitro immunization T2 - The journal of immunology N2 - Monoclonal antibodies are highly valuable tools in biomedicine but the generation by hybridoma technology is very time-consuming and elaborate. In order to circumvent the consisting drawbacks an in vitro immunization approach was established by which murine as well as human monoclonal antibodies against a viral coat protein could be developed. The in vitro immunization process was performed by isolation of murine hematopoietic stem cells or human monocytes and an in vitro differentiation into immature dendritic cells. After antigen loading the cells were co-cultivated with naive T and B lymphocytes for three days in order to obtain antigen-specific B lymphocytes in culture, followed by fusion with murine myeloma cells or human/murine heteromyeloma cells. Antigen-specific hybridomas were selected and the generated antibodies were purified and characterized in this study by ELISA, western blot, gene sequencing, affinity measurements. Further the characteristics were compared to a monoclonal antibody against the same target generated by conventional hybridoma technology. Isotype detection revealed a murine IgM and a human IgG4 antibody in comparison to an IgG1 for the conventionally generated antibody. The antibodies derived from in vitro immunization showed indeed a lower affinity for the antigen as compared to the conventionally generated one, which is probably based on the significantly shorter B cell maturation (3 days) during the immunization process. Nevertheless, they were suitable for building up a sandwich based detection system. Therefore, the in vitro immunization approach seems to be a good and particularly fast alternative to conventional hybridoma technology. Y1 - 2016 SN - 0022-1767 SN - 1550-6606 VL - 196 PB - American Assoc. of Immunologists CY - Bethesda ER - TY - GEN A1 - Holzlöhner, Pamela A1 - Butze, Monique A1 - Hebel, Nicole A1 - Weschke, Daniel A1 - Schliebs, E. A1 - Naumann, F. A1 - Füner, J. A1 - Micheel, Burkhard A1 - Hanack, Katja T1 - Monoclonal mouse antibodies against PBMC subpopulations of New World camelides T2 - European journal of immunology Y1 - 2016 SN - 0014-2980 SN - 1521-4141 VL - 46 SP - 1175 EP - 1175 PB - Wiley-Blackwell CY - Hoboken ER - TY - GEN A1 - Lechon, Tamara A1 - Sanz, Luis A1 - Pollmann, Stephan A1 - Sauer, Michael A1 - Sandalio, Luisa A1 - Lorenzo, Oscar T1 - Nitric oxide modification of plant endocytosis and PIN1 localization T2 - New biotechnology Y1 - 2016 U6 - https://doi.org/10.1016/j.nbt.2015.10.028 SN - 1871-6784 SN - 1876-4347 VL - 33 SP - 424 EP - 424 PB - Elsevier CY - Amsterdam ER - TY - GEN A1 - Hartmann, Matthias A1 - Fischer, Martin H. T1 - Exploring the numerical mind by eye-tracking: a special issue T2 - Psychological research : an international journal of perception, attention, memory, and action Y1 - 2016 U6 - https://doi.org/10.1007/s00426-016-0759-0 SN - 0340-0727 SN - 1430-2772 VL - 80 SP - 325 EP - 333 PB - Springer CY - Heidelberg ER - TY - GEN A1 - Bijleveld, Catrien A1 - Zoutewelle-Terovan, Mioara A1 - Huschek, Doreen A1 - Liefbroer, Aart C. T1 - Criminal careers and demographic outcomes: An introduction to the special issue T2 - Advances in life course research Y1 - 2016 U6 - https://doi.org/10.1016/j.alcr.2016.05.001 SN - 1569-4909 SN - 1879-6974 VL - 28 SP - 1 EP - 5 PB - Elsevier CY - Oxford ER - TY - GEN A1 - Leminen, Alina A1 - Lehtonen, Minna A1 - Bozic, Mirjana A1 - Clahsen, Harald T1 - Editorial: Morphologically Complex Words in the Mind/Brain T2 - Frontiers in human neuroscienc KW - morphology KW - derivation KW - inflection KW - compound KW - L2 KW - dyslexia KW - semantics KW - decomposition Y1 - 2016 U6 - https://doi.org/10.3389/fnhum.2016.00047 SN - 1662-5161 VL - 10 SP - 150 EP - 160 PB - Frontiers Research Foundation CY - Lausanne ER -