TY  - JOUR
A1  - Heise, Christian
A1  - Bier, Frank Fabian
T1  - Immobilization of DNA on microarrays
N2  - Microarrays are new analytical devices that allow the parallel and simultaneous detection of thousands of target compounds. Microarrays, also called DNA chips, are widely used in gene expression, the genotyping of individuals, point mutations, detection of single nucleotide polymorphisms, and short tandem repeats. Microarrays have highly specific base-pair interactions with labeled complementary strands, which makes this technology to a powerful analytical device for monitoring whole genomes. In this article, we provide a survey of the common microarray manufacturing methods, from the selection of support material to surface structuring, immobilization and hybridization, and finally the detection with labeled complementary strands. Special attention is given to the immobilization of single strands, since fast chemical reactions, the creation of homogeneous surface functionalities as well as an oriented coupling are crucial pre-conditions for a good spot morphology and microarrays of high quality
Y1  - 2005
ER  - 
TY  - JOUR
A1  - Steffen, Jenny
A1  - von Nickisch-Rosenegk, Markus
A1  - Bier, Frank Fabian
T1  - In vitro transcription of a whole gene on a surface-coupled template
N2  - An artificial gene was constructed combining the T7 promoter and terminator with the EGFP-gene from the plasmid pEGFP. The functionality of the construct was shown by in vitro translation. The gene-construct was immobilised on a planar glass surface. The transcription was performed on the immobilised gene and mRNA was determined by RT-PCR. Multiple use of the immobilised gene was demonstrated
Y1  - 2005
SN  - 1473-0189
ER  -