TY - JOUR A1 - Ogunkola, Moses Olalekan A1 - Guiraudie-Capraz, Gaelle A1 - Féron, François A1 - Leimkühler, Silke T1 - The Human Mercaptopyruvate Sulfurtransferase TUM1 Is Involved in Moco Biosynthesis, Cytosolic tRNA Thiolation and Cellular Bioenergetics in Human Embryonic Kidney Cells JF - Biomolecules N2 - Sulfur is an important element that is incorporated into many biomolecules in humans. The incorporation and transfer of sulfur into biomolecules is, however, facilitated by a series of different sulfurtransferases. Among these sulfurtransferases is the human mercaptopyruvate sulfurtransferase (MPST) also designated as tRNA thiouridine modification protein (TUM1). The role of the human TUM1 protein has been suggested in a wide range of physiological processes in the cell among which are but not limited to involvement in Molybdenum cofactor (Moco) biosynthesis, cytosolic tRNA thiolation and generation of H2S as signaling molecule both in mitochondria and the cytosol. Previous interaction studies showed that TUM1 interacts with the L-cysteine desulfurase NFS1 and the Molybdenum cofactor biosynthesis protein 3 (MOCS3). Here, we show the roles of TUM1 in human cells using CRISPR/Cas9 genetically modified Human Embryonic Kidney cells. Here, we show that TUM1 is involved in the sulfur transfer for Molybdenum cofactor synthesis and tRNA thiomodification by spectrophotometric measurement of the activity of sulfite oxidase and liquid chromatography quantification of the level of sulfur-modified tRNA. Further, we show that TUM1 has a role in hydrogen sulfide production and cellular bioenergetics. KW - Moco biosynthesis KW - sulfite oxidase KW - cytosolic tRNA thiolation KW - 5-methoxycarbonylmethyl-2-thiouridine KW - H2S biosynthesis KW - cellular bioenergetics Y1 - 2023 U6 - https://doi.org/10.3390/biom13010144 SN - 2218-273X VL - 13 SP - 1 EP - 23 PB - MDPI CY - Basel, Schweiz ET - 1 ER - TY - JOUR A1 - Marggraf, Lara Christin A1 - Lindecke, Oliver A1 - Voigt, Christian C. A1 - Pētersons, Gunārs A1 - Voigt-Heucke, Silke Luise T1 - Nathusius’ bats, Pipistrellus nathusii, bypass mating opportunities of their own species, but respond to foraging heterospecifics on migratory transit flights JF - Frontiers in Ecology and Evolution N2 - In late summer, migratory bats of the temperate zone face the challenge of accomplishing two energy-demanding tasks almost at the same time: migration and mating. Both require information and involve search efforts, such as localizing prey or finding potential mates. In non-migrating bat species, playback studies showed that listening to vocalizations of other bats, both con-and heterospecifics, may help a recipient bat to find foraging patches and mating sites. However, we are still unaware of the degree to which migrating bats depend on con-or heterospecific vocalizations for identifying potential feeding or mating opportunities during nightly transit flights. Here, we investigated the vocal responses of Nathusius’ pipistrelle bats, Pipistrellus nathusii, to simulated feeding and courtship aggregations at a coastal migration corridor. We presented migrating bats either feeding buzzes or courtship calls of their own or a heterospecific migratory species, the common noctule, Nyctalus noctula. We expected that during migratory transit flights, simulated feeding opportunities would be particularly attractive to bats, as well as simulated mating opportunities which may indicate suitable roosts for a stopover. However, we found that when compared to the natural silence of both pre-and post-playback phases, bats called indifferently during the playback of conspecific feeding sounds, whereas P. nathusii echolocation call activity increased during simulated feeding of N. noctula. In contrast, the call activity of P. nathusii decreased during the playback of conspecific courtship calls, while no response could be detected when heterospecific call types were broadcasted. Our results suggest that while on migratory transits, P. nathusii circumnavigate conspecific mating aggregations, possibly to save time or to reduce the risks associated with social interactions where aggression due to territoriality might be expected. This avoidance behavior could be a result of optimization strategies by P. nathusii when performing long-distance migratory flights, and it could also explain the lack of a response to simulated conspecific feeding. However, the observed increase of activity in response to simulated feeding of N. noctula, suggests that P. nathusii individuals may be eavesdropping on other aerial hawking insectivorous species during migration, especially if these occupy a slightly different foraging niche. KW - playback KW - phonotaxis KW - bats KW - acoustic communication KW - animal migration KW - eavesdropping KW - echolocation KW - Pipistrellus nathusii Y1 - 2023 U6 - https://doi.org/10.3389/fevo.2022.908560 SN - 2296-701X SP - 1 EP - 10 PB - Frontiers CY - Lausanne, Schweiz ER - TY - JOUR A1 - Kaak, Heinrich T1 - Chronologie der Englandreise JF - Ich gestehe, daß ich mich sehr bestimmt auf diese Reise freue … : Die Englandbriefe des märkischen Ehepaares von Itzenplitz 1792/1793 Y1 - 2023 SN - 978-3-86732-359-8 SP - 45 EP - 53 PB - Lukas CY - Berlin ER - TY - JOUR A1 - Kaak, Heinrich T1 - Einleitender Kommentar JF - Ich gestehe, daß ich mich sehr bestimmt auf diese Reise freue … : Die Englandbriefe des märkischen Ehepaares von Itzenplitz 1792/1793 Y1 - 2023 SN - 978-3-86732-359-8 SP - 11 EP - 44 PB - Lukas CY - Berlin ER - TY - JOUR A1 - Fenn, Monika A1 - Zülsdorf-Kersting, Meik T1 - Historisches Denken, historisches Wissen, historische Kompetenzen JF - Geschichts-Didaktik - Praxishandbuch für den Geschichtsunterricht : Sekundarstufe I und II Y1 - 2023 SN - 978-3-589-16886-6 SP - 53 PB - Cornelsen CY - Berlin ER - TY - JOUR A1 - Brinkmann, Pia A1 - Köllner, Nicole A1 - Merk, Sven A1 - Beitz, Toralf A1 - Altenberger, Uwe A1 - Löhmannsröben, Hans-Gerd T1 - Comparison of handheld and echelle spectrometer to assess copper in ores by means of laser-induced breakdown spectroscopy (LIBS) JF - Minerals N2 - Its properties make copper one of the world’s most important functional metals. Numerous megatrends are increasing the demand for copper. This requires the prospection and exploration of new deposits, as well as the monitoring of copper quality in the various production steps. A promising technique to perform these tasks is Laser Induced Breakdown Spectroscopy (LIBS). Its unique feature, among others, is the ability to measure on site without sample collection and preparation. In this work, copper-bearing minerals from two different deposits are studied. The first set of field samples come from a volcanogenic massive sulfide (VMS) deposit, the second part from a stratiform sedimentary copper (SSC) deposit. Different approaches are used to analyze the data. First, univariate regression (UVR) is used. However, due to the strong influence of matrix effects, this is not suitable for the quantitative analysis of copper grades. Second, the multivariate method of partial least squares regression (PLSR) is used, which is more suitable for quantification. In addition, the effects of the surrounding matrices on the LIBS data are characterized by principal component analysis (PCA), alternative regression methods to PLSR are tested and the PLSR calibration is validated using field samples. KW - LIBS KW - copper-bearing minerals KW - UVR KW - PCA KW - PLSR Y1 - 2023 U6 - https://doi.org/10.3390/min13010113 SN - 2075-163X VL - 13 IS - 1 PB - MDPI CY - Basel ER - TY - JOUR A1 - D'Aprile, Iwan-Michelangelo T1 - Bäcker Roesike statt Humboldt BT - Theodor Fontane, die Revolution und die Demokratie - Vorwort JF - Nur in Freiheit wird man frei Y1 - 2023 SN - 978-3-462-50002-8 SN - 978-3-462-51002-7 SP - 7 EP - 20 PB - Kiepenheuer & Witsch CY - Köln ER - TY - JOUR A1 - Albrecht, Anna Helena T1 - Meinungsfreiheit und Persönlichkeitsrecht in der neueren Rechtsprechung des Bundesverfassungsgerichts – Klarstellung, Konkretisierung oder Nachjustierung? JF - Zeitschrift für Urheber- und Medienrecht N2 - Das Spannungsverhältnis zwischen Meinungsfreiheit und allgemeinem Persönlichkeitsrecht hat das Bundesverfassungsgericht seit Jahrzehnten und wiederholt beschäftigt. Insbesondere in den letzten drei Jahren hatte sich das Gericht erneut intensiv damit zu befassen, einen angemessenen Ausgleich zwischen den sich gegenüberstehenden Grundrechtspositionen zu formulieren. Der Beitrag analysiert überblicksartig, inwieweit es in seinen neueren Entscheidungen seine bisherige Rechtsprechung fortgeführt, ergänzt oder gar nachjustiert hat. Y1 - 2023 SN - 0177-6762 IS - 1 SP - 8 EP - 15 PB - Nomos ER - TY - JOUR A1 - Faber, Eike ED - Rink, Martin ED - Haug, Clemens ED - Hammerich, Helmuth R. T1 - Der nördliche Schwarzmeerraum in der Antike JF - Ukraine und Ostmitteleuropa Y1 - 2023 UR - https://zms.bundeswehr.de/resource/blob/5588072/c693a0a201fb24f4c9274ce07ed99dfc/wegweiser-ukraine-und-ostmitteleuropa-data.pdf SN - 978-3-506-79153-5 SP - 28 EP - 37 PB - Brill CY - Paderborn ER - TY - JOUR A1 - Rashidi, Laleh T1 - Iran - das Pulverfass explodiert JF - Welttrends : das außenpolitische Journal Y1 - 2023 SN - 978-3-949887-06-2 SN - 0944-8101 VL - 31 IS - 195 SP - 48 EP - 53 PB - WeltTrends CY - Potsdam ER -