TY  - GEN
A1  - Meyer, Matthias
A1  - Palkopoulou, Eleftheria
A1  - Baleka, Sina Isabelle
A1  - Stiller, Mathias
A1  - Penkman, Kirsty E. H.
A1  - Alt, Kurt W.
A1  - Ishida, Yasuko
A1  - Mania, Dietrich
A1  - Mallick, Swapan
A1  - Meijer, Tom
A1  - Meller, Harald
A1  - Nagel, Sarah
A1  - Nickel, Birgit
A1  - Ostritz, Sven
A1  - Rohland, Nadin
A1  - Schauer, Karol
A1  - Schüler, Tim
A1  - Roca, Alfred L.
A1  - Reich, David
A1  - Shapiro, Beth
A1  - Hofreiter, Michael
T1  - Palaeogenomes of Eurasian straight-tusked elephants challenge the current view of elephant evolution
T2  - Postprints der Universität Potsdam Mathematisch-Naturwissenschaftliche Reihe
N2  - The straight-tusked elephants Palaeoloxodon spp. were widespread across Eurasia during the Pleistocene. Phylogenetic reconstructions using morphological traits have grouped them with Asian elephants (Elephas maximus), and many paleontologists place Palaeoloxodon within Elephas. Here, we report the recovery of full mitochondrial genomes from four and partial nuclear genomes from two P. antiquus fossils. These fossils were collected at two sites in Germany, Neumark-Nord and Weimar-Ehringsdorf, and likely date to interglacial periods similar to 120 and similar to 244 thousand years ago, respectively. Unexpectedly, nuclear and mitochondrial DNA analyses suggest that P. antiquus was a close relative of extant African forest elephants (Loxodonta cyclotis). Species previously referred to Palaeoloxodon are thus most parsimoniously explained as having diverged from the lineage of Loxodonta, indicating that Loxodonta has not been constrained to Africa. Our results demonstrate that the current picture of elephant evolution is in need of substantial revision.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 790 
KW  - genome sequence
KW  - woolly mammoth
KW  - Palaeoloxodon-antiquus
KW  - phylogenetic analysis
KW  - African elephants
KW  - DNA
KW  - Pleistocene
KW  - alignment
KW  - ancient
KW  - reveal
Y1  - 2019
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-440139
SN  - 1866-8372
IS  - 790
ER  - 
TY  - GEN
A1  - Kasyanenko, Nina
A1  - Unksov, Ivan
A1  - Bakulev, Vladimir
A1  - Santer, Svetlana
T1  - DNA interaction with head-to-tail associates of cationic surfactants prevents formation of compact particles
T2  - Postprints der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Cationic azobenzene-containing surfactants are capable of condensing DNA in solution with formation of nanosized particles that can be employed in gene delivery. The ratio of surfactant/DNA concentration and solution ionic strength determines the result of DNA-surfactant interaction: Complexes with a micelle-like surfactant associates on DNA, which induces DNA shrinkage, DNA precipitation or DNA condensation with the emergence of nanosized particles. UV and fluorescence spectroscopy, low gradient viscometry and flow birefringence methods were employed to investigate DNA-surfactant and surfactant-surfactant interaction at different NaCl concentrations, [NaCl]. It was observed that [NaCl] (or the Debye screening radius) determines the surfactant-surfactant interaction in solutions without DNA. Monomers, micelles and non-micellar associates of azobenzene-containing surfactants with head-to-tail orientation of molecules were distinguished due to the features of their absorption spectra. The novel data enabled us to conclude that exactly the type of associates (together with the concentration of components) determines the result of DNA-surfactant interaction. Predomination of head-to-tail associates at 0.01 M < [NaCl] < 0.5 M induces DNA aggregation and in some cases DNA precipitation. High NaCl concentration (higher than 0.8 M) prevents electrostatic attraction of surfactants to DNA phosphates for complex formation. DAPI dye luminescence in solutions with DNA-surfactant complexes shows that surfactant tails overlap the DNA minor groove. The addition of di- and trivalent metal ions before and after the surfactant binding to DNA indicate that the bound surfactant molecules are located on DNA in islets
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 940 
KW  - azobenzene trimethylammonium bromide
KW  - head-to-tail surfactant associates
KW  - DNA
KW  - ionic strength
KW  - multivalent ions
Y1  - 2020
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-459806
SN  - 1866-8372
IS  - 940
ER  -