TY  - JOUR
A1  - Forlani, Fabio
A1  - Cereda, Angelo
A1  - Freuer, Andrea
A1  - Nimtz, Manfred
A1  - Leimkühler, Silke
A1  - Pagani, Silvia
T1  - The cysteine-desulfurase IscS promotes the production of the rhodanese RhdA in the persulfurated form
N2  - After heterologous expression in Escherichia coli, the Azotobacter vinelandii rhodanese RhdA is purified in a persulfurated form (RhdA-SSH). We identified L-cysteine as the most effective sulfur source in producing RhdA-SSH. An E. coli soluble extract was required for in vitro persulfuration of RhdA, and the addition of pyridoxal-5'-phosphate increased RhdA-SSH production, indicating a likely involvement of a cysteine desulfurase. We were able to show the formation of a covalent complex between IscS and RhdA. By combining a time-course fluorescence assay and mass spectrometry analysis, we demonstrated the transfer of sulfur from E. coli IscS to RhdA. (c) 2005 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved
Y1  - 2005
SN  - 0014-5793
ER  -