TY  - JOUR
A1  - Breuer, David
A1  - Nowak, Jacqueline
A1  - Ivakov, Alexander
A1  - Somssich, Marc
A1  - Persson, Staffan
A1  - Nikoloski, Zoran
T1  - System-wide organization of actin cytoskeleton determines organelle transport in hypocotyl plant cells
JF  - Proceedings of the National Academy of Sciences of the United States of America
N2  - The actin cytoskeleton is an essential intracellular filamentous structure that underpins cellular transport and cytoplasmic streaming in plant cells. However, the system-level properties of actin-based cellular trafficking remain tenuous, largely due to the inability to quantify key features of the actin cytoskeleton. Here, we developed an automated image-based, network-driven framework to accurately segment and quantify actin cytoskeletal structures and Golgi transport. We show that the actin cytoskeleton in both growing and elongated hypocotyl cells has structural properties facilitating efficient transport. Our findings suggest that the erratic movement of Golgi is a stable cellular phenomenon that might optimize distribution efficiency of cell material. Moreover, we demonstrate that Golgi transport in hypocotyl cells can be accurately predicted from the actin network topology alone. Thus, our framework provides quantitative evidence for system-wide coordination of cellular transport in plant cells and can be readily applied to investigate cytoskeletal organization and transport in other organisms.
KW  - actin
KW  - cytoskeleton
KW  - Golgi
KW  - image processing
KW  - networks
Y1  - 2017
U6  - https://doi.org/10.1073/pnas.1706711114
SN  - 0027-8424
VL  - 114
SP  - E5741
EP  - E5749
PB  - National Acad. of Sciences
CY  - Washington
ER  -