TY  - JOUR
A1  - Oloya, Benson
A1  - Namukobe, Jane
A1  - Heydenreich, Matthias
A1  - Ssengooba, Willy
A1  - Schmidt, Bernd
A1  - Byamukama, Robert
T1  - Antimycobacterial activity of the extract and isolated compounds from the stem bark of Zanthoxylum leprieurii Guill. and Perr.
JF  - Natural product communications : an international journal for communications and reviews
N2  - Zanthoxylum leprieurii Guill. and Perr. (Rutaceae) stem bark is used locally in Uganda for treating tuberculosis (TB) and cough-related infections. Lupeol (1), sesamin (2), trans-fagaramide (3), arnottianamide (4), (S)-marmesinin (5), and hesperidin (6) were isolated from the chloroform/methanol (1:1) extract of Z. leprieurii stem bark. Their structures were elucidated using spectroscopic techniques and by comparison with literature data. Furthermore, the extract and isolated compounds were subjected to antimycobacterial activity. The extract exhibited moderate activity against the susceptible (H(37)Rv) TB strain, but weak activity against the multidrug resistant (MDR)-TB strain with minimum inhibitory concentrations (MICs) of 586.0 and 1172.0 mu g/mL, respectively. Compound 3 (trans-fagaramide) showed significant antimycobacterial activity against the susceptible (H(37)Rv) TB strain (MIC 6 mu g/mL), but moderate activity against the MDR-TB strain (MIC 12.2 mu g/mL). Compounds 2, 5, 6, and 1 showed moderate activities against the susceptible (H(37)Rv) strain (MIC 12.2-98.0 mu g/mL) and moderate to weak activities against the MDR-TB strain (MIC 24.4-195.0 mu g/mL). This study reports for the first time the isolation of compounds 1 to 6 from the stem bark of Z leprieurii. trans-Fagaramide (3) may present a vital template in pursuit of novel and highly effective TB drugs.
KW  - tuberculosis
KW  - Zanthoxylum leprieurii
KW  - antimycobacterial activity
KW  - trans-fagaramide
KW  - alkaloid
Y1  - 2021
U6  - https://doi.org/10.1177/1934578X211035851
SN  - 1934-578X
SN  - 1555-9475
VL  - 16
IS  - 8
PB  - Sage Publ.
CY  - Thousand Oaks
ER  -