TY  - JOUR
A1  - Mehr, Fatemeh Naderi
A1  - Grigoriev, Dmitry
A1  - Heaton, Rebecca
A1  - Baptiste, Joshua
A1  - Stace, Anthony J.
A1  - Puretskiy, Nikolay
A1  - Besley, Elena
A1  - Böker, Alexander
T1  - Self-assembly behavior of oppositely charged inverse bipatchy microcolloids
JF  - Small : nano micro
N2  - A directed attractive interaction between predefined "patchy" sites on the surfaces of anisotropic microcolloids can provide them with the ability to self-assemble in a controlled manner to build target structures of increased complexity. An important step toward the controlled formation of a desired superstructure is to identify reversible electrostatic interactions between patches which allow them to align with one another. The formation of bipatchy particles with two oppositely charged patches fabricated using sandwich microcontact printing is reported. These particles spontaneously self-aggregate in solution, where a diversity of short and long chains of bipatchy particles with different shapes, such as branched, bent, and linear, are formed. Calculations show that chain formation is driven by a combination of attractive electrostatic interactions between oppositely charged patches and the charge-induced polarization of interacting particles.
KW  - electrostatic interactions
KW  - patchy particles
KW  - polyelectrolyte inks
KW  - sandwich microcontact printing
KW  - self-assembly
Y1  - 2020
U6  - https://doi.org/10.1002/smll.202000442
SN  - 1613-6810
SN  - 1613-6829
VL  - 16
IS  - 14
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Kochovski, Zdravko
A1  - Chen, Guosong
A1  - Yuan, Jiayin
A1  - Lu, Yan
T1  - Cryo-Electron microscopy for the study of self-assembled poly(ionic liquid) nanoparticles and protein supramolecular structures
JF  - Colloid and polymer science : official journal of the Kolloid-Gesellschaft
N2  - Cryo-electron microscopy (cryo-EM) is a powerful structure determination technique that is well-suited to the study of protein and polymer self-assembly in solution. In contrast to conventional transmission electron microscopy (TEM) sample preparation, which often times involves drying and staining, the frozen-hydrated sample preparation allows the specimens to be kept and imaged in a state closest to their native one. Here, we give a short overview of the basic principles of Cryo-EM and review our results on applying it to the study of different protein and polymer self-assembled nanostructures. More specifically, we show how we have applied cryo-electron tomography (cryo-ET) to visualize the internal morphology of self-assembled poly(ionic liquid) nanoparticles and cryo-EM single particle analysis (SPA) to determine the three-dimensional (3D) structures of artificial protein microtubules.
KW  - self-assembly
KW  - poly(ionic liquid) nanoparticles
KW  - protein self-assembly
KW  - cryo-electron microscopy
KW  - single particle analysis
KW  - cryo-electron
KW  - tomography
Y1  - 2020
U6  - https://doi.org/10.1007/s00396-020-04657-w
SN  - 0303-402X
SN  - 1435-1536
VL  - 298
IS  - 7
SP  - 707
EP  - 717
PB  - Springer
CY  - New York
ER  -