TY  - JOUR
A1  - Reinicke, Stefan
A1  - Fischer, Thilo
A1  - Bramski, Julia
A1  - Pietruszka, Jörg
A1  - Böker, Alexander
T1  - Biocatalytically active microgels by precipitation polymerization of N-isopropyl acrylamide in the presence of an enzyme
JF  - RSC Advances
N2  - We present a novel protocol for the synthesis of enzymatically active microgels. The protocol is based on the precipitation polymerization of N-isopropylacrylamide (NIPAm) in the presence of an enzyme and a protein binding comonomer. A basic investigation on the influence of different reaction parameters such as monomer concentration and reaction temperature on the microgel size and size distribution is performed and immobilization yields are determined. Microgels exhibiting hydrodynamic diameters between 100 nm and 1 mu m and narrow size distribution could be synthesized while about 31-44% of the enzyme present in the initial reaction mixture can be immobilized. Successful immobilization including a verification of enzymatic activity of the microgels is achieved for glucose oxidase (GOx) and 2-deoxy-d-ribose-5-phosphate aldolase (DERA). The thermoresponsive properties of the microgels are assessed and discussed in the light of activity evolution with temperature. The positive correlation of enzymatic activity with temperature for the GOx containing microgel originates from a direct interaction of the enzyme with the PNIPAm based polymer matrix whose magnitude is highly influenced by temperature.
Y1  - 2019
U6  - https://doi.org/10.1039/c9ra04000e
SN  - 2046-2069
VL  - 9
IS  - 49
SP  - 28377
EP  - 28386
PB  - Royal Society of Chemistry
CY  - Cambridge
ER  -