TY  - JOUR
A1  - Schulze, Sven
A1  - Wehrhold, Michel
A1  - Hille, Carsten
T1  - Femtosecond-Pulsed laser written and etched fiber bragg gratings for fiber-optical biosensing
JF  - Sensors
N2  - We present the development of a label-free, highly sensitive fiber-optical biosensor for online detection and quantification of biomolecules. Here, the advantages of etched fiber Bragg gratings (eFBG) were used, since they induce a narrowband Bragg wavelength peak in the reflection operation mode. The gratings were fabricated point-by-point via a nonlinear absorption process of a highly focused femtosecond-pulsed laser, without the need of prior coating removal or specific fiber doping. The sensitivity of the Bragg wavelength peak to the surrounding refractive index (SRI), as needed for biochemical sensing, was realized by fiber cladding removal using hydrofluoric acid etching. For evaluation of biosensing capabilities, eFBG fibers were biofunctionalized with a single-stranded DNA aptamer specific for binding the C-reactive protein (CRP). Thus, the CRP-sensitive eFBG fiber-optical biosensor showed a very low limit of detection of 0.82 pg/L, with a dynamic range of CRP detection from approximately 0.8 pg/L to 1.2 mu g/L. The biosensor showed a high specificity to CRP even in the presence of interfering substances. These results suggest that the proposed biosensor is capable for quantification of CRP from trace amounts of clinical samples. In addition, the adaption of this eFBG fiber-optical biosensor for detection of other relevant analytes can be easily realized.
KW  - fiber Bragg gratings
KW  - ultra-fast laser inscription
KW  - fiber etching
KW  - nanostructure fabrication
KW  - fiber-optical sensors
KW  - aptamers
KW  - C-reactive protein
KW  - biomarker
Y1  - 2018
U6  - https://doi.org/10.3390/s18092844
SN  - 1424-8220
VL  - 18
IS  - 9
PB  - MDPI
CY  - Basel
ER  - 
TY  - JOUR
A1  - Schulze-Makuch, Dirk
A1  - Wagner, Dirk
A1  - Kounaves, Samuel P.
A1  - Mangelsdorf, Kai
A1  - Devine, Kevin G.
A1  - de Vera, Jean-Pierre
A1  - Schmitt-Kopplin, Philippe
A1  - Grossart, Hans-Peter
A1  - Parro, Victor
A1  - Kaupenjohann, Martin
A1  - Galy, Albert
A1  - Schneider, Beate
A1  - Airo, Alessandro
A1  - Froesler, Jan
A1  - Davila, Alfonso F.
A1  - Arens, Felix L.
A1  - Caceres, Luis
A1  - Cornejo, Francisco Solis
A1  - Carrizo, Daniel
A1  - Dartnell, Lewis
A1  - DiRuggiero, Jocelyne
A1  - Flury, Markus
A1  - Ganzert, Lars
A1  - Gessner, Mark O.
A1  - Grathwohl, Peter
A1  - Guan, Lisa
A1  - Heinz, Jacob
A1  - Hess, Matthias
A1  - Keppler, Frank
A1  - Maus, Deborah
A1  - McKay, Christopher P.
A1  - Meckenstock, Rainer U.
A1  - Montgomery, Wren
A1  - Oberlin, Elizabeth A.
A1  - Probst, Alexander J.
A1  - Saenz, Johan S.
A1  - Sattler, Tobias
A1  - Schirmack, Janosch
A1  - Sephton, Mark A.
A1  - Schloter, Michael
A1  - Uhl, Jenny
A1  - Valenzuela, Bernardita
A1  - Vestergaard, Gisle
A1  - Woermer, Lars
A1  - Zamorano, Pedro
T1  - Transitory microbial habitat in the hyperarid Atacama Desert
JF  - Proceedings of the National Academy of Sciences of the United States of America
KW  - habitat
KW  - aridity
KW  - microbial activity
KW  - biomarker
KW  - Mars
Y1  - 2018
U6  - https://doi.org/10.1073/pnas.1714341115
SN  - 0027-8424
VL  - 115
IS  - 11
SP  - 2670
EP  - 2675
PB  - National Acad. of Sciences
CY  - Washington
ER  -