TY  - JOUR
A1  - Gendre, Delphine
A1  - Baral, Anirban
A1  - Dang, Xie
A1  - Esnay, Nicolas
A1  - Boutte, Yohann
A1  - Stanislas, Thomas
A1  - Vain, Thomas
A1  - Claverol, Stephane
A1  - Gustavsson, Anna
A1  - Lin, Deshu
A1  - Grebe, Markus
A1  - Bhalerao, Rishikesh P.
T1  - Rho-of-plant activated root hair formation requires Arabidopsis YIP4a/b gene function
JF  - Development : Company of Biologists
N2  - Root hairs are protrusions from root epidermal cells with crucial roles in plant soil interactions. Although much is known about patterning, polarity and tip growth of root hairs, contributions of membrane trafficking to hair initiation remain poorly understood. Here, we demonstrate that the trans-Golgi network-localized YPT-INTERACTING PROTEIN 4a and YPT-INTERACTING PROTEIN 4b (YIP4a/b) contribute to activation and plasma membrane accumulation of Rho-of-plant (ROP) small GTPases during hair initiation, identifying YIP4a/b as central trafficking components in ROP-dependent root hair formation.
KW  - ROP
KW  - YIP
KW  - Root hair
KW  - Secretion
KW  - Trans-Golgi network
Y1  - 2019
U6  - https://doi.org/10.1242/dev.168559
SN  - 0950-1991
SN  - 1477-9129
VL  - 146
IS  - 5
PB  - The Company of Biologists
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Kiefer, Christian S.
A1  - Claes, Andrea R.
A1  - Nzayisenga, Jean-Claude
A1  - Pietra, Stefano
A1  - Stanislas, Thomas
A1  - Ikeda, Yoshihisa
A1  - Grebe, Markus
T1  - Arabidopsis AIP1-2 restricted by WER-mediated patterning modulates planar polarity
JF  - Development
N2  - The coordination of cell polarity within the plane of the tissue layer (planar polarity) is crucial for the development of diverse multicellular organisms. Small Rac/Rho-family GTPases and the actin cytoskeleton contribute to planar polarity formation at sites of polarity establishment in animals and plants. Yet, upstream pathways coordinating planar polarity differ strikingly between kingdoms. In the root of Arabidopsis thaliana, a concentration gradient of the phytohormone auxin coordinates polar recruitment of Rho-of-plant (ROP) to sites of polar epidermal hair initiation. However, little is known about cytoskeletal components and interactions that contribute to this planar polarity or about their relation to the patterning machinery. Here, we show that ACTIN7 (ACT7) represents a main actin isoform required for planar polarity of root hair positioning, interacting with the negative modulator ACTIN-INTERACTING PROTEIN1-2 (AIP1-2). ACT7, AIP1-2 and their genetic interaction are required for coordinated planar polarity of ROP downstream of ethylene signalling. Strikingly, AIP1-2 displays hair cell file-enriched expression, restricted by WEREWOLF (WER)-dependent patterning and modified by ethylene and auxin action. Hence, our findings reveal AIP1-2, expressed under control of the WER-dependent patterning machinery and the ethylene signalling pathway, as a modulator of actin-mediated planar polarity.
KW  - AIP1
KW  - Actin
KW  - Arabidopsis
KW  - Patterning
KW  - Planar polarity
Y1  - 2015
UR  - http://dev.biologists.org/content/142/1/151.long
U6  - https://doi.org/doi: 10.1242/dev.111013
IS  - 142
SP  - 151
EP  - 161
ER  - 
TY  - JOUR
A1  - Stanislas, Thomas
A1  - Huser, Anke
A1  - Barbosa, Ines C. R.
A1  - Kiefer, Christian S.
A1  - Brackmann, Klaus
A1  - Pietra, Stefano
A1  - Gustavsson, Anna
A1  - Zourelidou, Melina
A1  - Schwechheimer, Claus
A1  - Grebe, Markus
T1  - Arabidopsis D6PK is a lipid domain-dependent mediator of root epidermal planar polarity
JF  - Nature plants
N2  - Development of diverse multicellular organisms relies on coordination of single-cell polarities within the plane of the tissue layer (planar polarity). Cell polarity often involves plasma membrane heterogeneity generated by accumulation of specific lipids and proteins into membrane subdomains. Coordinated hair positioning along Arabidopsis root epidermal cells provides a planar polarity model in plants, but knowledge about the functions of proteo-lipid domains in planar polarity signalling remains limited. Here we show that Rho-of-plant (ROP) 2 and 6, phosphatidylinositol-4-phosphate 5-kinase 3 (PIP5K3), DYNAMIN-RELATED PROTEIN (DRP) 1A and DRP2B accumulate in a sterol-enriched, polar membrane domain during root hair initiation. DRP1A, DRP2B, PIP5K3 and sterols are required for planar polarity and the AGCVIII kinase D6 PROTEIN KINASE (D6PK) is a modulator of this process. D6PK undergoes phosphatidylinositol-4,5-bisphosphate- and sterol-dependent basal-to-planar polarity switching into the polar, lipid-enriched domain just before hair formation, unravelling lipid-dependent D6PK localization during late planar polarity signalling.
Y1  - 2015
U6  - https://doi.org/10.1038/NPLANTS.2015.162
SN  - 2055-026X
SN  - 2055-0278
VL  - 1
IS  - 11
PB  - Nature Publ. Group
CY  - London
ER  - 
TY  - JOUR
A1  - Kiefer, Christian S.
A1  - Claes, Andrea R.
A1  - Nzayisenga, Jean-Claude
A1  - Pietra, Stefano
A1  - Stanislas, Thomas
A1  - Hueser, Anke
A1  - Ikeda, Yoshihisa
A1  - Grebe, Markus
T1  - Arabidopsis AIP1-2 restricted by WER-mediated patterning modulates planar polarity
JF  - Development : Company of Biologists
N2  - The coordination of cell polarity within the plane of the tissue layer (planar polarity) is crucial for the development of diverse multicellular organisms. Small Rac/Rho-family GTPases and the actin cytoskeleton contribute to planar polarity formation at sites of polarity establishment in animals and plants. Yet, upstream pathways coordinating planar polarity differ strikingly between kingdoms. In the root of Arabidopsis thaliana, a concentration gradient of the phytohormone auxin coordinates polar recruitment of Rho-of-plant (ROP) to sites of polar epidermal hair initiation. However, little is known about cytoskeletal components and interactions that contribute to this planar polarity or about their relation to the patterning machinery. Here, we show that ACTIN7 (ACT7) represents a main actin isoform required for planar polarity of root hair positioning, interacting with the negative modulator ACTIN-INTERACTING PROTEIN1-2 (AIP1-2). ACT7, AIP1-2 and their genetic interaction are required for coordinated planar polarity of ROP downstream of ethylene signalling. Strikingly, AIP1-2 displays hair cell file-enriched expression, restricted by WEREWOLF (WER)-dependent patterning and modified by ethylene and auxin action. Hence, our findings reveal AIP1-2, expressed under control of the WER-dependent patterning machinery and the ethylene signalling pathway, as a modulator of actin-mediated planar polarity.
KW  - AIP1
KW  - Arabidopsis
KW  - WEREWOLF
KW  - Actin
KW  - Patterning
KW  - Planar polarity
Y1  - 2015
U6  - https://doi.org/10.1242/dev.111013
SN  - 0950-1991
SN  - 1477-9129
VL  - 142
IS  - 1
SP  - 151
EP  - 161
PB  - Company of Biologists Limited
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Frescatada-Rosa, Marcia
A1  - Stanislas, Thomas
A1  - Backues, Steven K.
A1  - Reichardt, Ilka
A1  - Men, Shuzhen
A1  - Boutte, Yohann
A1  - Juergens, Gerd
A1  - Moritz, Thomas
A1  - Bednarek, Sebastian York
A1  - Grebe, Markus
T1  - High lipid order of Arabidopsis cell-plate membranes mediated by sterol and Dynamin-Related Protein 1A function
JF  - The plant journal
N2  - Membranes of eukaryotic cells contain high lipid-order sterol-rich domains that are thought to mediate temporal and spatial organization of cellular processes. Sterols are crucial for execution of cytokinesis, the last stage of cell division, in diverse eukaryotes. The cell plate of higher-plant cells is the membrane structure that separates daughter cells during somatic cytokinesis. Cell-plate formation in Arabidopsis relies on sterol- and DYNAMIN-RELATED PROTEIN1A (DRP1A)-dependent endocytosis. However, functional relationships between lipid membrane order or lipid packing and endocytic machinery components during eukaryotic cytokinesis have not been elucidated. Using ratiometric live imaging of lipid order-sensitive fluorescent probes, we show that the cell plate of Arabidopsis thaliana represents a dynamic, high lipid-order membrane domain. The cell-plate lipid order was found to be sensitive to pharmacological and genetic alterations of sterol composition. Sterols co-localize with DRP1A at the cell plate, and DRP1A accumulates in detergent-resistant membrane fractions. Modifications of sterol concentration or composition reduce cell-plate membrane order and affect DRP1A localization. Strikingly, DRP1A function itself is essential for high lipid order at the cell plate. Our findings provide evidence that the cell plate represents a high lipid-order domain, and pave the way to explore potential feedback between lipid order and function of dynamin-related proteins during cytokinesis.
KW  - membrane order
KW  - sterol
KW  - cytokinesis
KW  - DRP1A
KW  - Arabidopsis
Y1  - 2014
U6  - https://doi.org/10.1111/tpj.12674
SN  - 0960-7412
SN  - 1365-313X
VL  - 80
IS  - 5
SP  - 745
EP  - 757
PB  - Wiley-Blackwell
CY  - Hoboken
ER  -