TY  - JOUR
A1  - Hovestaedt, Marc
A1  - Memczak, Henry
A1  - Pleiner, Dennis
A1  - Zhang, Xin
A1  - Rappich, Joerg
A1  - Bier, Frank Fabian
A1  - Stöcklein, Walter F. M.
T1  - Characterization of a new maleimido functionalization of gold for surface plasmon resonance spectroscopy
JF  - Journal of molecular recognition : an international journal devoted to research on specific molecular recognition in chemistry, biology, biotechnology and medicine
N2  - Para-maleimidophenyl (p-MP) modified gold surfaces have been prepared by one-step electrochemical deposition and used in surface plasmon resonance (SPR) studies. Therefore, a FITC mimotope peptide (MP1, 12 aa), a human mucin 1 epitope peptide (MUC, 9 aa) and a protein with their specific antibodies were used as model systems. The peptides were modified with an N-terminal cysteine for covalent and directed coupling to the maleimido functionalized surface by means of Michael addition. The coupling yield of the peptide, the binding characteristics of antibody and the unspecific adsorption of the analytes were investigated. The results expand the spectrum of biosensors usable with p-MP by widely used SPR and support its potential to be versatile for several electrochemical and optical biosensors. This allows the combination of an electrochemical and optical read-out for a broad variety of biomolecular interactions on the same chip. Copyright (c) 2014 John Wiley & Sons, Ltd.
KW  - biosensor
KW  - surface plasmon resonance
KW  - diazonium coupling
KW  - maleimidophenyl
KW  - cys-peptide
KW  - aryl diazonium salts
Y1  - 2014
U6  - https://doi.org/10.1002/jmr.2396
SN  - 0952-3499
SN  - 1099-1352
VL  - 27
IS  - 12
SP  - 707
EP  - 713
PB  - Wiley-Blackwell
CY  - Hoboken
ER  -