TY  - JOUR
A1  - Uribe, Veronica
A1  - Ramadass, Radhan
A1  - Dogra, Deepika
A1  - Rasouli, S. Javad
A1  - Gunawan, Felix
A1  - Nakajima, Hiroyuki
A1  - Chiba, Ayano
A1  - Reischauer, Sven
A1  - Mochizuki, Naoki
A1  - Stainier, Didier Y. R.
T1  - In vivo analysis of cardiomyocyte proliferation during trabeculation
JF  - Development : Company of Biologists
N2  - Cardiomyocyte proliferation is crucial for cardiac growth, patterning and regeneration; however, few studies have investigated the behavior of dividing cardiomyocytes in vivo. Here, we use time-lapse imaging of beating hearts in combination with the FUCCI system to monitor the behavior of proliferating cardiomyocytes in developing zebrafish. Confirming in vitro observations, sarcomere disassembly, as well as changes in cell shape and volume, precede cardiomyocyte cytokinesis. Notably, cardiomyocytes in zebrafish embryos and young larvae mostly divide parallel to the myocardial wall in both the compact and trabecular layers, and cardiomyocyte proliferation is more frequent in the trabecular layer. While analyzing known regulators of cardiomyocyte proliferation, we observed that the Nrg/ErbB2 and TGF beta signaling pathways differentially affect compact and trabecular layer cardiomyocytes, indicating that distinct mechanisms drive proliferation in these two layers. In summary, our data indicate that, in zebrafish, cardiomyocyte proliferation is essential for trabecular growth, but not initiation, and set the stage to further investigate the cellular and molecular mechanisms driving cardiomyocyte proliferation in vivo.
KW  - Heart development
KW  - Cardiomyocyte proliferation
KW  - Trabeculation
KW  - Sarcomere
Y1  - 2018
U6  - https://doi.org/10.1242/dev.164194
SN  - 0950-1991
SN  - 1477-9129
VL  - 145
IS  - 14
PB  - Company biologists LTD
CY  - Cambridge
ER  - 
TY  - JOUR
A1  - Bornhorst, Dorothee
A1  - Xia, Peng
A1  - Nakajima, Hiroyuki
A1  - Dingare, Chaitanya
A1  - Herzog, Wiebke
A1  - Lecaudey, Virginie
A1  - Mochizuki, Naoki
A1  - Heisenberg, Carl-Philipp
A1  - Yelon, Deborah
A1  - Abdelilah-Seyfried, Salim
T1  - Biomechanical signaling within the developing zebrafish heart attunes endocardial growth to myocardial chamber dimensions
JF  - Nature Communications
N2  - Intra-organ communication guides morphogenetic processes that are essential for an organ to carry out complex physiological functions. In the heart, the growth of the myocardium is tightly coupled to that of the endocardium, a specialized endothelial tissue that lines its interior. Several molecular pathways have been implicated in the communication between these tissues including secreted factors, components of the extracellular matrix, or proteins involved in cell-cell communication. Yet, it is unknown how the growth of the endocardium is coordinated with that of the myocardium. Here, we show that an increased expansion of the myocardial atrial chamber volume generates higher junctional forces within endocardial cells. This leads to biomechanical signaling involving VE-cadherin, triggering nuclear localization of the Hippo pathway transcriptional regulator Yap1 and endocardial proliferation. Our work suggests that the growth of the endocardium results from myocardial chamber volume expansion and ends when the tension on the tissue is relaxed.
Y1  - 2019
U6  - https://doi.org/10.1038/s41467-019-12068-x
SN  - 2041-1723
VL  - 10
PB  - Nature Publ. Group
CY  - London
ER  -