TY  - GEN
A1  - Schlör, Anja
A1  - Hirschberg, Stefan
A1  - Ben Amor, Ghada
A1  - Meister, Toni Luise
A1  - Arora, Prerna
A1  - Pöhlmann, Stefan
A1  - Hoffmann, Markus
A1  - Pfänder, Stephanie
A1  - Eddin, Omar Kamal
A1  - Kamhieh-Milz, Julian
A1  - Hanack, Katja
T1  - SARS-CoV-2 neutralizing camelid heavy-chain-only antibodies as powerful tools for diagnostic and therapeutic applications
T2  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe
N2  - Introduction: The ongoing COVID-19 pandemic situation caused by SARS-CoV-2 and variants of concern such as B.1.617.2 (Delta) and recently, B.1.1.529 (Omicron) is posing multiple challenges to humanity. The rapid evolution of the virus requires adaptation of diagnostic and therapeutic applications.

Objectives: In this study, we describe camelid heavy-chain-only antibodies (hcAb) as useful tools for novel in vitro diagnostic assays and for therapeutic applications due to their neutralizing capacity.

Methods: Five antibody candidates were selected out of a naïve camelid library by phage display and expressed as full length IgG2 antibodies. The antibodies were characterized by Western blot, enzyme-linked immunosorbent assays, surface plasmon resonance with regard to their specificity to the recombinant SARS-CoV-2 Spike protein and to SARS-CoV-2 virus-like particles. Neutralization assays were performed with authentic SARS-CoV-2 and pseudotyped viruses (wildtype and Omicron).

Results: All antibodies efficiently detect recombinant SARS-CoV-2 Spike protein and SARS-CoV-2 virus-like particles in different ELISA setups. The best combination was shown with hcAb B10 as catcher antibody and HRP-conjugated hcAb A7.2 as the detection antibody. Further, four out of five antibodies potently neutralized authentic wildtype SARS-CoV-2 and particles pseudotyped with the SARS-CoV-2 Spike proteins of the wildtype and Omicron variant, sublineage BA.1 at concentrations between 0.1 and 0.35 ng/mL (ND50).

Conclusion: Collectively, we report novel camelid hcAbs suitable for diagnostics and potential therapy.
T3  - Zweitveröffentlichungen der Universität Potsdam : Mathematisch-Naturwissenschaftliche Reihe - 1280 
KW  - camelid heavy-chain-only antibodies
KW  - single domain antibodies
KW  - nanobodies
KW  - SARS-CoV-2
KW  - neutralization
KW  - Omicron
Y1  - 2022
U6  - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus4-570124
SN  - 1866-8372
IS  - 1280
ER  - 
TY  - JOUR
A1  - Schlör, Anja
A1  - Hirschberg, Stefan
A1  - Ben Amor, Ghada
A1  - Meister, Toni Luise
A1  - Arora, Prerna
A1  - Pöhlmann, Stefan
A1  - Hoffmann, Markus
A1  - Pfänder, Stephanie
A1  - Eddin, Omar Kamal
A1  - Kamhieh-Milz, Julian
A1  - Hanack, Katja
T1  - SARS-CoV-2 neutralizing camelid heavy-chain-only antibodies as powerful tools for diagnostic and therapeutic applications
JF  - Frontiers in Immunology
N2  - Introduction: The ongoing COVID-19 pandemic situation caused by SARS-CoV-2 and variants of concern such as B.1.617.2 (Delta) and recently, B.1.1.529 (Omicron) is posing multiple challenges to humanity. The rapid evolution of the virus requires adaptation of diagnostic and therapeutic applications.

Objectives: In this study, we describe camelid heavy-chain-only antibodies (hcAb) as useful tools for novel in vitro diagnostic assays and for therapeutic applications due to their neutralizing capacity.

Methods: Five antibody candidates were selected out of a naïve camelid library by phage display and expressed as full length IgG2 antibodies. The antibodies were characterized by Western blot, enzyme-linked immunosorbent assays, surface plasmon resonance with regard to their specificity to the recombinant SARS-CoV-2 Spike protein and to SARS-CoV-2 virus-like particles. Neutralization assays were performed with authentic SARS-CoV-2 and pseudotyped viruses (wildtype and Omicron).

Results: All antibodies efficiently detect recombinant SARS-CoV-2 Spike protein and SARS-CoV-2 virus-like particles in different ELISA setups. The best combination was shown with hcAb B10 as catcher antibody and HRP-conjugated hcAb A7.2 as the detection antibody. Further, four out of five antibodies potently neutralized authentic wildtype SARS-CoV-2 and particles pseudotyped with the SARS-CoV-2 Spike proteins of the wildtype and Omicron variant, sublineage BA.1 at concentrations between 0.1 and 0.35 ng/mL (ND50).

Conclusion: Collectively, we report novel camelid hcAbs suitable for diagnostics and potential therapy.
KW  - camelid heavy-chain-only antibodies
KW  - single domain antibodies
KW  - nanobodies
KW  - SARS-CoV-2
KW  - neutralization
KW  - Omicron
Y1  - 2022
U6  - https://doi.org/10.3389/fimmu.2022.930975
SN  - 1664-3224
SP  - 1
EP  - 14
PB  - Frontiers Media SA
CY  - Lausanne, Schweiz
ER  -