TY  - JOUR
A1  - Pecher, Gabriele
A1  - Harnack, U.
A1  - Gunther, M.
A1  - Hummel, M.
A1  - Fichtner, I.
A1  - Schenk, Jörg A.
T1  - Generation of an immortalized human CD4+ T cell clone inhibiting tumor growth in mice.
N2  - Tumor antigen-specific T cell clones represent a useful tool in tumor immunology; however, their long-term culture is limited. To generate an immortalized cytotoxic T cell clone against the human tumor antigen mucin, we exposed a previously generated T cell culture to Herpesvirus saimiri. We obtained an immortalized human CD4+ T cell clone, termed SITAM. Clonality of these cells was shown by analysis of the alpha/beta-T cell receptor (TCR) repertoire. Cytolytic activity was demonstrated against several mucin-expressing tumor cell lines and could not be detected against non-mucin-expressing cells. SITAM cells maintained their features stably for 2 years. Furthermore, growth of the tumor cell line Capan-2 in NOD/SCID mice was inhibited when SITAM cells were coinjected subcutaneously with tumor cells. SITAM cells provide an unlimited source of clonal T cells for analysis of tumor recognition and may be of help in TCR-targeted immunotherapy.
Y1  - 2001
UR  - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-45S4JD7- VM&_coverDate=05%2F18%2F2001&_alid=268965202&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=6713&_sort=d&view=c&_acct=C000053886&_ v
ER  -