TY  - JOUR
A1  - Griessner, Matthias
A1  - Hartig, Dave
A1  - Christmann, Alexander
A1  - Pohl, Carsten
A1  - Schellhase, Michaela
A1  - Ehrentreich-Förster, Eva
T1  - Development and characterization of a disposable plastic microarray printhead
JF  - Biomedical microdevices : bioMEMS and biomedical nanotechnology
N2  - During the last decade microarrays have become a powerful analytical tool. Commonly microarrays are produced in a non-contact manner using silicone printheads. However, silicone printheads are expensive and not able to be used as a disposable. Here, we show the development and functional characterization of 8-channel plastic microarray printheads that overcome both disadvantages of their conventional silicone counterparts. A combination of injection-molding and laser processing allows us to produce a high quantity of cheap, customizable and disposable microarray printheads. The use of plastics (e.g., polystyrene) minimizes the need for surface modifications required previously for proper printing results. Time-consuming regeneration processes, cleaning procedures and contaminations caused by residual samples are avoided. The utilization of plastic printheads for viscous liquids, such as cell suspensions or whole blood, is possible. Furthermore, functional parts within the plastic printhead (e.g., particle filters) can be included. Our printhead is compatible with commercially available TopSpot devices but provides additional economic and technical benefits as compared to conventional TopSpot printheads, while fulfilling all requirements demanded on the latter. All in all, this work describes how the field of traditional microarray spotting can be extended significantly by low cost plastic printheads.
KW  - Microarray
KW  - Printhead
KW  - Disposable
KW  - Plastics
KW  - Injection-molding
Y1  - 2011
U6  - https://doi.org/10.1007/s10544-011-9522-x
SN  - 1387-2176
VL  - 13
IS  - 3
SP  - 533
EP  - 538
PB  - Springer
CY  - Dordrecht
ER  - 
TY  - JOUR
A1  - Grießner, Matthias
A1  - Hartig, Dave
A1  - Christmann, Alexander
A1  - Ehrentreich-Förster, Eva
A1  - Warsinke, Axel
A1  - Bier, Frank Fabian
T1  - Surface regeneration of microfluidic microarray printheads through plasma techniques
N2  - This work describes a method for surface regeneration of microfluidic microarray printheads through plasma techniques. Modification procedures were chosen in a way to obtain high reproducibility with a minimum of time consumption. The idea behind this is a complete regeneration of a microarray printhead before or after usage to achieve best printing results over a typical print job. A sequence of low-pressure oxygen-plasma and plasma polymerization with hexamethyldisiloxane (HMDSO) was used to regenerate printheads. Proof of the concept is given through quality control performed with a spotter implemented CCD camera, contact angle measurements and a typical hybridization experiment. Stable printing results were obtained over 3000 activations showing that the presented method is suitable for treatment of microarray printheads.
Y1  - 2010
UR  - http://iopscience.iop.org/0960-1317/
U6  - https://doi.org/10.1088/0960-1317/20/3/037002
SN  - 0960-1317
ER  -