TY  - JOUR
A1  - Grießner, Matthias
A1  - Broeker, Patrick
A1  - Lehmann, André
A1  - Ehrentreich-Förster, Eva
A1  - Bier, Frank Fabian
T1  - Detection of angiotensin II type 1 receptor ligands by a cell-based assay
N2  - This work describes a cell-based assay that does not depend on radioactivity or laboratory animals for the detection of ligands of angiotensin II type 1 receptor (AT(1)R). The assay makes use of stable transfected Chinese hamster ovary cells (CHO-AT(1)R) expressing the AT(1)R. A sequential saturation assay principle was used in which receptor binding sites of the CHO-AT(1)R cells are blocked by the analyte in a concentration-dependent manner. Afterwards, TAMRA-angiotensin II, a fluorescence-labeled ligand, was added to bind to the remaining free binding sites of the receptor. In consequence, the fluorescence signal determined is inversely proportional to the concentration of the analyte.
Y1  - 2009
UR  - http://www.springerlink.com/content/100417
U6  - https://doi.org/10.1007/s00216-009-3074-4
SN  - 1618-2642
ER  -