TY - JOUR A1 - Sellrie, Frank A1 - Schenk, Jörg A. A1 - Behrsing, Olaf A1 - Bottger, Volker A1 - Micheel, Burkhard T1 - A competitive immunoassay to detect a hapten using an enzyme-labelled peptide mimotope as tracer N2 - Mimotope peptides-peptides which mimic the binding of a hapten to its corresponding monoclonal antibody-were conjugated to peroxidase and used in competitive immunoassay. The established immunoassay was used to quantitatively determine the concentration of hapten. As model system in all the experiments described here, we used the binding of the monoclonal antibody B13-DE1 to fluorescein and the corresponding peptide mimotope. Y1 - 2002 UR - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T2Y-44MGNDF- 1&_coverDate=03%2F01%2F2002&_alid=268992656&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=4931&_sort=d&view=c&_acct=C000053886&_v e ER - TY - CHAP A1 - Le, Dinh To A1 - Behrsing, Olaf A1 - Rothschild, Claire A1 - Radukic, Marco T. A1 - Arndt, Katja A1 - Müller, Kristian M. T1 - AAV capsid proteins fused with SARS-CoV-2 RBD or RBM: Expression in E. coli, in-vitro assembly, and characterization BT - 24th Annual Meeting of the American Society of Gene & Cell Therapy : Complete listing of the accepted abstracts for presentation at ASGCT's 24th Annual Meeting, May 11-May 14, 2021 T2 - Molecular therapy : the journal of the American Society of Gene Therapy Y1 - 2021 U6 - https://doi.org/10.1016/j.ymthe.2021.04.019 SN - 1525-0016 SN - 1525-0024 VL - 29 IS - 4, Suppl. 1 SP - 357 EP - 357 PB - Cell Press CY - Cambridge ER - TY - JOUR A1 - Sellrie, Frank A1 - Schenk, Jörg A. A1 - Behrsing, Olaf A1 - Drechsel, Oliver A1 - Micheel, Burkhard T1 - Cloning and characterization of a single chain antibody to glucose oxidase from a murine hybridoma N2 - Glucose oxidase (GOD) is an oxidoreductase catalyzing the reaction of glucose and oxygen to peroxide and gluconolacton (EC 1.1.3.4.). GOD is a widely used enzyme in biotechnology. Therefore the production of monoclonal antibodies and antibody fragments to GOD are of interest in bioanalytics and even tumor therapy. We describe here the generation of a panel of monoclonal antibodies to native and heat inactivated GOD. One of the hybridomas, E13BC8, was used for cloning of a single chain antibody (scFv). This scFv was expressed in Escherichia coli XL1-blue with the help of the vector system pOPE101. The scFv was isolated from the periplasmic fraction and detected by western blotting. It reacts specifically with soluble active GOD but does not recognize denatured GOD adsorbed to the solid phase. The same binding properties were also found for the monoclonal antibody E13BC8. Y1 - 2007 UR - http://www.jbmb.or.kr/fulltext/jbmb/view.php?vol=40&page=875 ER - TY - JOUR A1 - Stöcklein, Walter F. M. A1 - Behrsing, Olaf A1 - Scharte, Gudrun A1 - Micheel, Burkhard A1 - Benkert, Alexander A1 - Schössler, W. A1 - Warsinke, Axel A1 - Scheller, Frieder W. T1 - Enzyme kinetic assays with surface plasmon resonance (BIAcore) based on competition between enzyme and creatinine antibody Y1 - 2000 ER - TY - JOUR A1 - Kneissel, Sandra A1 - Queitsch, Iris A1 - Petersen, Gabriele A1 - Behrsing, Olaf A1 - Micheel, Burkhard A1 - Düberl, Stefan T1 - Epitope structures recognised by antibodies against the major coat protein (g8p) of filamentous bacteriophage fd (Inoviridae) Y1 - 1999 ER - TY - JOUR A1 - Gedvilaite, Alma A1 - Frömmel, C. A1 - Sasnauskas, K. A1 - Micheel, Burkhard A1 - Özel, M. A1 - Behrsing, Olaf A1 - Staniulis, J. A1 - Jandrig, Burkhard A1 - Scherneck, Siegfried A1 - Ulrich, R. T1 - Formation of immunogenic virus-like particles by inserting epitopes into surface-exposed regions of hamster polyomavirus major capsid protein Y1 - 2000 ER - TY - JOUR A1 - Werner, Deljana A1 - Behrsing, Olaf A1 - Scharte, Gudrun A1 - Woller, Jochen A1 - Steup, Martin A1 - Micheel, Burkhard T1 - Monoclonal anti-diuron antibodies prevent inhibition of photosynthesis by diuron Y1 - 2002 ER - TY - JOUR A1 - Behrsing, Olaf A1 - Micheel, Burkhard T1 - Monoklonale Antikörper : Grundlagen und ihre Bedeutung in Diagnostik und Therapie Y1 - 2008 SN - 978-3-540-69412-0 ER - TY - JOUR A1 - Rohde, M. A1 - Schenk, Jörg A. A1 - Heymann, Stephan A1 - Behrsing, Olaf A1 - Scharte, Gudrun A1 - Kempter, Gerhard A1 - Woller, Jochen A1 - Höhne, Wolfgang A1 - Warsinke, Axel A1 - Micheel, Burkhard T1 - Production and characterization of monoclonal antibodeis against urea derivatives Y1 - 1998 ER - TY - JOUR A1 - Stöcklein, Walter F. M. A1 - Rohde, M. A1 - Scharte, Gudrun A1 - Behrsing, Olaf A1 - Warsinke, Axel A1 - Micheel, Burkhard A1 - Scheller, Frieder W. T1 - Sensitive detection of triazine and phenylurea pesticides in pure organic solvent by enzyme linked immunsorbent assay (ELISA): stabilities, solubilities and sensitives Y1 - 2000 ER -