TY  - JOUR
A1  - Pitzen, Valentin
A1  - Askarzada, Sophie
A1  - Gräf, Ralph
A1  - Meyer, Irene
T1  - CDK5RAP2 Is an Essential Scaffolding Protein of the Corona of the Dictyostelium Centrosome
JF  - Cells
N2  - Dictyostelium centrosomes consist of a nucleus-associated cylindrical, three-layered core structure surrounded by a corona consisting of microtubule-nucleation complexes embedded in a scaffold of large coiled-coil proteins. One of them is the conserved CDK5RAP2 protein. Here we focus on the role of Dictyostelium CDK5RAP2 for maintenance of centrosome integrity, its interaction partners and its dynamic behavior during interphase and mitosis. GFP-CDK5RAP2 is present at the centrosome during the entire cell cycle except from a short period during prophase, correlating with the normal dissociation of the corona at this stage. RNAi depletion of CDK5RAP2 results in complete disorganization of centrosomes and microtubules suggesting that CDK5RAP2 is required for organization of the corona and its association to the core structure. This is in line with the observation that overexpressed GFP-CDK5RAP2 elicited supernumerary cytosolic MTOCs. The phenotype of CDK5RAP2 depletion was very reminiscent of that observed upon depletion of CP148, another scaffolding protein of the corona. BioID interaction assays revealed an interaction of CDK5RAP2 not only with the corona markers CP148, gamma-tubulin, and CP248, but also with the core components Cep192, CP75, and CP91. Furthermore, protein localization studies in both depletion strains revealed that CP148 and CDK5RAP2 cooperate in corona organization.
KW  - centrosome
KW  - centriole
KW  - Dictyostelium
KW  - microtubules
KW  - mitosis
Y1  - 2018
U6  - https://doi.org/10.3390/cells7040032
SN  - 2073-4409
VL  - 7
IS  - 4
PB  - MDPI
CY  - Basel
ER  -