TY  - JOUR
A1  - Micheel, Burkhard
T1  - Monoklonale Antikörper
Y1  - 2003
ER  - 
TY  - JOUR
A1  - Warsinke, Axel
A1  - Lettau, Kristian
A1  - Werner, Deljana
A1  - Micheel, Burkhard
A1  - Kwak, Young-Keun
T1  - Biomimetic Binders and Catalysts for Sensorics = Biomimetische Binder und Katalysatoren für die Sensorik
N2  - Biosensors which make use of the high specificity of enzymes, antibodies, and nucleic acids have been described for detection of numerous metabolites, hormones, and nucleic acid sequences. In addition to biological components nowadays biomimetic recognition molecules are also used. Especially antibodies, aptamers, and molecular imprints are promising biomimetics. They could broaden the range of detectable analytes and could increase the functional stability of the sensor. In this publication we describe the generation of biomimetic antibodies and biomimetic molecular imprints for binding creatinine and for hydrolyzing phenylcarbamates to be used in electrochemical sensors.
Y1  - 2003
UR  - http://www.extenza-eps.com/extenza/loadHTML?objectIDValue=21853&type=abstract
ER  - 
TY  - JOUR
A1  - Sheriff, Ahmed
A1  - Vogt, B.
A1  - Baumgart, Martin
A1  - Montag, C.
A1  - Hollenbach, B.
A1  - Schenk, Jörg A.
A1  - Ulrich, J.
A1  - Ellias, F.
A1  - Micheel, Burkhard
T1  - Intracellular capture of B7 in antigen-presenting cells reduces costimulatory activity
N2  - CTLA-4 gene constructs were designed to express CTLA-4 exclusively in the endoplasmic reticulum (ER). Four different CTLA-4 gene constructs were transfected into HEK 293 (human embryonic kidney) and A20 (Balb/c mouse B lymphoma) cells. All constructs contained an ER retention signal and coded for CTLA-4 expression in the ER. One of the constructs, which contained the membrane part of CTLA-4, coded for an expression both on the cell surface and in the ER. Three of the expressed CTLA-4 types (including the ER-membrane-expressed form) caused a reduced surface expression of B7 in the A20 cells. Only constructs which allow dimerization of CTLA-4 showed this effect. It is assumed that intracellular CTLA-4 bound B7 and inhibited therefore the transport of B7 to the surface. The binding obviously caused also an enhanced degradation of the complexes because both proteins showed a low concentration in the transfected cell lines. CTLA-4-transfected and B7-reduced A20 cells showed a diminished costimulating activity upon T cells. This was demonstrated by a reduced proliferation of T cells from ovalbumin-immunized Balb/c mice, incubated with ovalbumin peptide-primed CTLA-4-transfected A20 cells.
Y1  - 2003
UR  - http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WBK-47T23XK- 8&_coverDate=02%2F21%2F2003&_alid=268969757&_rdoc=1&_fmt=&_orig=search&_qd=1&_cdi=6713&_sort=d&view=c&_acct=C000053886&_v e
ER  -