TY  - JOUR
A1  - Wurzbacher, Christian
A1  - Warthmann, Norman
A1  - Bourne, Elizabeth Charlotte
A1  - Attermeyer, Katrin
A1  - Allgaier, Martin
A1  - Powell, Jeff R.
A1  - Detering, Harald
A1  - Mbedi, Susan
A1  - Großart, Hans-Peter
A1  - Monaghan, Michael T.
T1  - High habitat-specificity in fungal communities in oligo-mesotrophic, temperate Lake Stechlin (North-East Germany)
JF  - MycoKeys
N2  - Freshwater fungi are a poorly studied ecological group that includes a high taxonomic diversity. Most studies on aquatic fungal diversity have focused on single habitats, thus the linkage between habitat heterogeneity and fungal diversity remains largely unexplored. We took 216 samples from 54 locations representing eight different habitats in the meso-oligotrophic, temperate Lake Stechlin in North-East Germany. These included the pelagic and littoral water column, sediments, and biotic substrates. We performed high throughput sequencing using the Roche 454 platform, employing a universal eukaryotic marker region within the large ribosomal subunit (LSU) to compare fungal diversity, community structure, and species turnover among habitats. Our analysis recovered 1027 fungal OTUs (97% sequence similarity). Richness estimates were highest in the sediment, biofilms, and benthic samples (189-231 OTUs), intermediate in water samples (42-85 OTUs), and lowest in plankton samples (8 OTUs). NMDS grouped the eight studied habitats into six clusters, indicating that community composition was strongly influenced by turnover among habitats. Fungal communities exhibited changes at the phylum and order levels along three different substrate categories from littoral to pelagic habitats. The large majority of OTUs (> 75%) could not be classified below the order level due to the lack of aquatic fungal entries in public sequence databases. Our study provides a first estimate of lake-wide fungal diversity and highlights the important contribution of habitat heterogeneity to overall diversity and community composition. Habitat diversity should be considered in any sampling strategy aiming to assess the fungal diversity of a water body.
KW  - Freshwater fungi
KW  - aquatic fungi
KW  - metabarcoding
KW  - LSU
KW  - GMYC
KW  - habitat specificity
KW  - Chytridiomycota
KW  - Cryptomycota
KW  - Rozellomycota
KW  - community ecology
KW  - lake ecosystem
KW  - biofilm
KW  - sediment
KW  - plankton
KW  - water sample
KW  - benthos
KW  - reed
KW  - fungal diversity
Y1  - 2016
U6  - https://doi.org/10.3897/mycokeys.16.9646
SN  - 1314-4057
SN  - 1314-4049
VL  - 41
SP  - 17
EP  - 44
PB  - Pensoft Publ.
CY  - Sofia
ER  - 
TY  - JOUR
A1  - Schroedter, Linda
A1  - Schneider, Roland
A1  - Remus, Lisa
A1  - Venus, Joachim
T1  - L-(+)-lactic acid from reed
BT  - comparing various resources for the nutrient provision of B. coagulans
JF  - Resources
N2  - Biotechnological production of lactic acid (LA) is based on the so-called first generation feedstocks, meaning sugars derived from food and feed crops such as corn, sugarcane and cassava. The aim of this study was to exploit the potential of a second generation resource: Common reed (Phragmites australis) is a powerfully reproducing sweet grass which grows in wetlands and creates vast monocultural populations. This lignocellulose biomass bears the possibility to be refined to value-added products, without competing with agro industrial land. Besides utilizing reed as a renewable and inexpensive substrate, low-cost nutritional supplementation was analyzed for the fermentation of thermophilicBacilluscoagulans.Various nutritional sources such as baker's and brewer's yeast, lucerne green juice and tryptone were investigated for the replacement of yeast extract. The structure of the lignocellulosic material was tackled by chemical treatment (1% NaOH) and enzymatic hydrolysis (Cellic(R)CTec2).B.coagulansDSM ID 14-300 was employed for the homofermentative conversion of the released hexose and pentose sugars to polymerizable L-(+)-LA of over 99.5% optical purity. The addition of autolyzed baker's yeast led to the best results of fermentation, enabling an LA titer of 28.3 g L(-1)and a yield of 91.6%.
KW  - lignocellulose
KW  - reed
KW  - Phragmites australis
KW  - lactic acid
KW  - Bacillus
KW  - coagulans
Y1  - 2020
U6  - https://doi.org/10.3390/resources9070089
SN  - 2079-9276
VL  - 9
IS  - 7
PB  - MDPI
CY  - Basel
ER  -