TY - JOUR A1 - Herbst, Uta A1 - Fuchs, Iris Judith A1 - Teubner, Wera A1 - Seidel, Albrecht A1 - Frank, Heinz A1 - Steinberg, Pablo T1 - Malignant transformation of human colon epithelial cells by polycyclic aromatic hydrocarbons and heterocyclic aromatic amines Y1 - 2004 SN - 0028-1298 ER - TY - JOUR A1 - Herbst, Uta A1 - Fuchs, Iris Judith A1 - Teubner, Wera A1 - Steinberg, Pablo T1 - Malignant transformation of human colon epithelial cells by benzo[c]phenanthrene dihydrodiolepoxides as well as 2-hydroxyamino-1-methyl-6-phenylimidazo[4,5-b]pyridine N2 - Polycyclic aromatic hydrocarbons (PAHs) and heterocyclic aromatic amines (HCAs) ingested with food have repeatedly been suggested to be involved in the malignant transformation of colon epithelial cells. In order to test this hypothesis, HCEC cells (SV40 large T antigen-immortalized human colon epithelial cells) were incubated with a racemic mixture of benzo[c]phenanthrene dihydrodiol epoxides (B[c]PhDE), extremely potent carcinogenic PAH metabolites in vivo, or with 2-hydroxyamino-1-methyl-6-phenylimidazo[4,5-b]pyridine (N-OH-PhIP), the N-hydroxylated metabolite of the most abundant HCA in cooked meat. First, it was shown that HCEC cells express sulfotransferase 1A1, which is needed to metabolize N-OH-PhIP to the corresponding N-sulfonyloxy derivative, the direct precursor molecule of genotoxic nitrenium ions. Thereafter, exponentially growing HCEC cells were exposed five times to 0.1 mu g (0.37 nmol) B[c]PhDE/ml for 30 min or 0.72 mu g (3 mnol) N-OH-PhTP/ml for 24 h. Chemically treated HCEC cells showed an enhanced saturation density and grew faster than the corresponding solvent-treated cell cultures. After five treatment cycles, HCECB[c]PhDE as well as HCECN-OH-PhIP cells lost cell-cell contact inhibition and started piling up and forming foci in the culture flasks. Furthermore, HCECB[c]phDE and HCECN-OH-PhIP cells were injected i.m. into SCID mice. Within 6 weeks after injection, eight animals out of eight injected with HCECB[c]phDE or HCECN-OH-PhIP cells developed tumors at the site of injection, thus demonstrating the high tumorigenic potential of the HCECB[c]PhDE and HCECN-OH-PhIP cell cultures. Taken together, we show for the first time that the abovementioned active PAH metabolites as well as N-OH-PhIP are indeed able to malignantly transform human colon epithelial cells in vitro. Y1 - 2006 UR - http://www.sciencedirect.com/science/journal/0041008X U6 - https://doi.org/10.1016/j.taap.2005.07.016 SN - 0041-008X ER - TY - THES A1 - Herbst, Uta T1 - Untersuchungen zur In-vitro-Zelltransformation in Dickdarmepithelzellen des Menschen und Dünndarmephithelzellen der Ratte durch Benzo(c)phenanthren-3,4-dihydrodiol-1,2-epoxide Y1 - 2012 CY - Potsdam ER - TY - BOOK A1 - Voeth, Markus A1 - Herbst, Uta A1 - Austen, Viola A1 - Becker, Tatjana A1 - Bertels, Victoria A1 - Heigl, Julia A1 - Huttelmaier, Hannes A1 - Kugler, Aline A1 - Loos, Jeanette A1 - Meister, Christoph A1 - Rentner, Björn A1 - Richter, Jenny A1 - Schmidt, Natalie A1 - Schwarz, Daniela A1 - schwarz, Sabine T1 - Marketing-Managment : Grundlagen, Konzeption und Umsetzung Y1 - 2013 SN - 978-3-7910-3271-9 PB - Schäffer-Poeschel Verlag CY - Stuttgart ER - TY - JOUR A1 - Vöth, Markus A1 - Herbst, Uta A1 - Liess, Frank T1 - We know exactly what you want the development of a completely individualised conjoint analysis JF - International journal of market research N2 - Improving the predictive validity of conjoint analysis has been an important research objective for many years. Whereas the majority of attempts have been different approaches to preference modelling, data collection or product presentation, only a few scholars have tried to improve predictive validity by individualising conjoint designs. This comes as a surprise because many markets have observed an augmented demand for customised products and highly heterogeneous customers' preferences. Against this background, the authors develop a conjoint variant based on a completely individualised conjoint design. More concretely, the new approach not only individualises the attributes, but also the attribute levels. The results of a comprehensive empirical study yield a significantly higher validity than existing standardised-level conjoint approaches. Consequently, they help marketers to gain deeper insights into their customers' preferences. Y1 - 2013 U6 - https://doi.org/10.2501/IJMR-2013-038 SN - 1470-7853 VL - 55 IS - 3 SP - 437 EP - 458 PB - Market Research Society CY - London ER - TY - BOOK A1 - Voeth, Markus A1 - Herbst, Uta T1 - Verhandlungsmanagement BT - Planung, Steuerung und Analyse N2 - In nahezu allen Unternehmensbereichen spielen Verhandlungen eine zentrale Rolle. - Umfassender Ansatz des betriebswirtschaftlichen Verhandlungsmanagements - Praxiserfahrungen - Aktuelle Erkenntnisse der Verhandlungsforschung - Instrumente und Tools zur Planung, Steuerung und Kontrolle - Realitätsnahe Fallstudien - Prägnante Beispiele - Übungsaufgaben Y1 - 2015 SN - 978-3-7910-3570-3 PB - Schäffer-Poeschel CY - Stuttgart ET - 2. völlig neu bearb. Auflage ER - TY - JOUR A1 - Herbst, Uta A1 - Wagner, Dieter T1 - Potsdam Transfer - die tentrale wissenschaftliche Einrichtung für Gründung, Innovation, Wissens- und Technologietransfer JF - Entrepreneurship education: das Potsdamer Modell der Gründungslehre und -beratung Y1 - 2015 SN - 978-3-7357-6095-1 SP - 46 EP - 52 PB - BoD CY - Norderstedt ER - TY - JOUR A1 - Herbst, Uta A1 - Kemmerling, Birte Christina A1 - Neale, Margaret A. T1 - All in, one-at-a-time or somewhere in the middle? BT - Leveraging the composition and size of the negotiating package JF - The journal of business & industrial marketing N2 - Purpose: While industrial marketers have long bundled their products and services to sell them as packages, to what extent should negotiators also rely on packaging their offers? Clearly, negotiating at a package level can tax the cognitive capacity of the involved parties at some point. Therefore, this study aims to analyze the impact of the number and type of issues that should be negotiated simultaneously to leverage the package strategy efficiently and effectively in multi-issue buyer-seller negotiations. Design/methodology/approach: The authors conducted and analyzed negotiation simulations with 676 students from 2 public universities. Findings: The authors’ results suggest that negotiating three out of six issues simultaneously is the least efficient but most effective strategy in multi-issue buyer-seller negotiations. Moreover, they found that bundling distributive and integrative issues is more efficient and effective than only bundling distributive or integrative negotiation issues in a package offer. Originality/value: Past research has examined the impact of negotiating a package as compared to each issue separately; however, little empirical attention has been directed toward understanding how to apply a package strategy in complex multi-issue negotiations. KW - Negotiation performance KW - Issue bundling KW - Joint negotiation outcome KW - Number of offers KW - Package strategy Y1 - 2017 U6 - https://doi.org/10.1108/JBIM-12-2015-0251 SN - 0885-8624 SN - 2052-1189 VL - 32 IS - 4 SP - 580 EP - 586 PB - Emerald Group Publishing Limited CY - Bingley ER - TY - JOUR A1 - Thaler, Verena Sabine A1 - Herbst, Uta A1 - Merz, Michael A. T1 - A real product scandal’s impact on a high-equity brand BT - a new approach to assessing scandal impact to assessing scandal impact JF - Journal of Product & Brand Management N2 - Findings - The results provide (longitudinal) support for the proposed evaluative approach. They reveal new evidence that building brand equity is a means to mitigate negative effects, and indicate that negative spillover effects within a high-equity brand portfolio are unlikely. Finally, this research identifies situations in which developing a new brand might be more beneficial than leveraging an existing brand. Practical implications - This research has significant implications for firms with high-equity brands that might be affected by a scandal. The findings support managers to navigate their brands through a crisis. KW - Crisis management KW - Consumer brand equity KW - Product scandal Y1 - 2018 U6 - https://doi.org/10.1108/JPBM-05-2017-1469 SN - 1061-0421 VL - 27 IS - 4 SP - 427 EP - 439 PB - Emerald Group Publishing Limited CY - Bingley ER - TY - JOUR A1 - Voeth, Markus A1 - Herbst, Uta A1 - Haggenmüller, Sandra A1 - Weber, Marie-Christin T1 - Wie verhandeln deutsche Manager? JF - Zeitschrift für Konfliktmanagement Y1 - 2019 U6 - https://doi.org/10.9785/zkm-2020-230107 VL - 23 IS - 1 SP - 21 EP - 24 PB - Dr. Otto Schmidt CY - Köln ER -