TY  - JOUR
A1  - Pearson, Leanne A.
A1  - Dittmann, Elke
A1  - Mazmouz, Rabia
A1  - Ongley, Sarah E.
A1  - Neilan, Brett A.
T1  - The genetics, biosynthesis and regulation of toxic specialized metabolites of cyanobacteria
JF  - Harmful algae
N2  - The production of toxic metabolites by cyanobacterial blooms represents a significant threat to the health of humans and ecosystems worldwide. Here we summarize the current state of the knowledge regarding the genetics, biosynthesis and regulation of well-characterized cyanotoxins, including the microcystins, nodularin, cylindrospermopsin, saxitoxins and antitoxins, as well as the lesser-known marine toxins (e.g. lyngbyatoxin, aplysiatoxin, jamaicamides, barbamide, curacin, hectochlorin and apratoxins). (C) 2015 Elsevier B.V. All rights reserved.
KW  - Cyanobacteria
KW  - Cyanotoxins
KW  - Specialized metabolites
KW  - Genetics
KW  - Biosynthesis
KW  - Regulation
Y1  - 2016
U6  - https://doi.org/10.1016/j.hal.2015.11.002
SN  - 1568-9883
SN  - 1878-1470
VL  - 54
SP  - 98
EP  - 111
PB  - Elsevier
CY  - Amsterdam
ER  - 
TY  - JOUR
A1  - Soeriyadi, Angela H.
A1  - Ongley, Sarah E.
A1  - Kehr, Jan-Christoph
A1  - Pickford, Russel
A1  - Dittmann, Elke
A1  - Neilan, Brett A.
T1  - Tailoring enzyme stringency masks the multispecificity of a lyngbyatoxin (indolactam alkaloid) nonribosomal peptide synthetase
JF  - ChemBioChem
N2  - Indolactam alkaloids are activators of protein kinase C (PKC) and are of pharmacological interest for the treatment of pathologies involving PKC dysregulation. The marine cyanobacterial nonribosomal peptide synthetase (NRPS) pathway for lyngbyatoxin biosynthesis, which we previously expressed in E. coli, was studied for its amenability towards the biosynthesis of indolactam variants. Modification of culture conditions for our E. coli heterologous expression host and analysis of pathway products suggested the native lyngbyatoxin pathway NRPS does possess a degree of relaxed specificity. Site-directed mutagenesis of two positions within the adenylation domain (A-domain) substrate-binding pocket was performed, resulting in an alteration of substrate preference between valine, isoleucine, and leucine. We observed relative congruence of in vitro substrate activation by the LtxA NRPS to in vivo product formation. While there was a preference for isoleucine over leucine, the substitution of alternative tailoring domains may unveil the true in vivo effects of the mutations introduced herein.
KW  - a domain
KW  - indolactams
KW  - MbtH
KW  - natural products
KW  - teleocidin
Y1  - 2021
U6  - https://doi.org/10.1002/cbic.202100574
SN  - 1439-4227
SN  - 1439-7633
VL  - 23
IS  - 3
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Woodhouse, Jason Nicholas
A1  - Makower, A. Katharina
A1  - Yeung, Anna C. Y.
A1  - Ongley, Sarah E.
A1  - Micallef, Melinda L.
A1  - Moffitt, Michelle C.
A1  - Neilan, Brett A.
T1  - Advances in genomics, transcriptomics and proteomics of toxin-producing cyanobacteria
JF  - Environmental microbiology reports
N2  - A common misconception persists that the genomes of toxic and non-toxic cyanobacterial strains are largely conserved with the exception of the presence or absence of the genes responsible for toxin production. Implementation of -omics era technologies has challenged this paradigm, with comparative analyses providing increased insight into the differences between strains of the same species. The implementation of genomic, transcriptomic and proteomic approaches has revealed distinct profiles between toxin-producing and non-toxic strains. Further, metagenomics and metaproteomics highlight the genomic potential and functional state of toxic bloom events over time. In this review, we highlight how these technologies have shaped our understanding of the complex relationship between these molecules, their producers and the environment at large within which they persist.
Y1  - 2016
U6  - https://doi.org/10.1111/1758-2229.12366
SN  - 1758-2229
VL  - 8
SP  - 3
EP  - 13
PB  - Wiley-Blackwell
CY  - Hoboken
ER  -