TY - JOUR A1 - Bathe, Katharina A1 - Chen, Frank A1 - Büscher, Ulrich A1 - Dudenhausen, Joachim W. A1 - Schweigert, Florian J. T1 - Veränderung der Carotinoidkonzentration im Serum und in der Milch im Verlauf der Laktation der Frau Y1 - 2000 ER - TY - BOOK A1 - Raila, Jens A1 - Wirth, Kerstin A1 - Büscher, Ulrich A1 - Dudenhausen, Joachim W. A1 - Schweigert, Florian J. T1 - Einfluss normaler und pathologischer Schwangerschaftsverläufe auf die renale Ausscheidung von Vitamin A und Retinol-Bindungsprotein Y1 - 2001 ER - TY - JOUR A1 - Raila, Jens A1 - Wirth, Kerstin A1 - Chen, Frank A1 - Büscer, Ulrich A1 - Dudenhausen, Joachim W. A1 - Schweigert, Florian J. T1 - Excretion of vitamin A in urine of women during Normal pregnancy and pregnancy complications N2 - Background/Aims: The renal function, including the excretion of low-molecular-weight proteins, changes during pregnancy and may cause a urinary excretion of retinol-binding protein (RBP). Whether it is accompanied by a substantial loss of vitamin A ( retinol) has not been established yet. We therefore determined the excretion of retinol and RBP in urine of pregnant women. Methods: The study involved analyses of urine samples from 40 healthy pregnant women and 29 women with pregnancy complications during the third trimester. Analyses of plasma and urine of 7 healthy women and 5 women with pregnancy complications were also carried out 6 weeks antepartum, at time of delivery and 1 week postpartum. Results: Urinary retinol was higher in women who suffered from pregnancy disorders with an influence on maternal metabolism ( p < 0.01). RBP was excreted at substantial concentrations in the urine of all 69 women, but there were no differences between the groups. Women with a concomitant excretion of retinol had higher levels of urinary RBP than those without a retinol excretion ( p < 0.05). Differences in plasma retinol and RBP were not significant. Conclusion: The excretion of urinary retinol may increase significantly during pregnancy complications, which needs further clarification to which extent this condition may negatively affect the vitamin A status in such women. Copyright (C) 2004 S. Karger AG, Basel Y1 - 2004 SN - 0250-6807 ER - TY - JOUR A1 - Schweigert, Florian J. A1 - Bathe, Katharina A1 - Chen, Frank A1 - Büscher, Ulrich A1 - Dudenhausen, Joachim W. T1 - Effect of the stage of lactation in humans on carotenoid levels in milk, blood plasma and plasma lipoprotein fractions N2 - In mammals the composition of milk changes during early lactation, with a rapid decline of fat-soluble vitamins and a continuous increase in total lipids. The mechanisms underlying this phenomenon are not well understood, but might involve selective mechanisms related to mammary uptake or secretion into the milk. Since carotenoids are specifically distributed among the lipoprotein fractions in plasma, the simultaneous determination of carotenoids in plasma, lipoprotein fractions and milk might offer an opportunity to gain insight into this phenomenon. In 21 healthy mothers carotenoids in plasma and lipoprotein fractions were investigated at day 2 and 19 and milk on day 4 and 19 after delivery. Plasma levels of alpha-tocopherol and cholesterol as well as lutein, zeaxanthin and cryptoxanthin were significantly lower later in lactation (day 19) than shortly after birth (P < 0.01). The stage of lactation had no effect on the distribution of carotenoids and -tocopherol among the plasma lipoprotein fractions. In milk, triacylglycerol increased (P < 0.01). In contrast, levels of carotenoids, alpha- tocopherol and vitamin A were highest in colostrum and declined (P < 0.01). Because the magnitude of decrease was not the same in all carotenoids, the carotenoid pattern changed substantially. In colostrum the carotenoid pattern resembled those of plasma and the low- density lipoprotein fraction. In mature milk it was similar to the pattern found in the high density lipoprotein fraction. Based on these observations a selective mechanism might be responsible for the transfer of these components in milk involving different lipoprotein fractions at specific times of lactation Y1 - 2004 ER - TY - JOUR A1 - Schweigert, Florian J. A1 - Gericke, Beate A1 - Wolfram, Wiebke A1 - Kaisers, Udo A1 - Dudenhausen, Joachim W. T1 - Peptide and protein profiles in serum and follicular fluid of women undergoing IVF JF - Human reproduction N2 - BACKGROUND: Proteins and peptides in human follicular fluid originate from plasma or are produced by follicular structures. Compositional changes reflect oocyte maturation and can be used as diagnostic markers. The aim of the study was to determine protein and peptide profiles in paired serum and follicular fluid samples from women undergoing IVF. METHODS: Surface-enhanced laser desorption and ionization-time of flight-mass spectrometry (SELDI-TOF-MS) was used to obtain characteristic protein pattern. RESULTS: One hundred and eighty-six individual MS signals were obtained from a combination of enrichment on strong anion exchanger (110), weak cation exchanger (52) and normal phase surfaces (24). On the basis of molecular masses, isoelectric points and immunoreactivety, four signals were identified as haptoglobin (alpha(1)- and alpha(2)-chain), haptoglobin 1 and transthyretin (TTR). Immunological and MS characteristics of the TTR : retinol-binding protein (RBP) transport complex revealed no microheterogeneity differences between serum and follicular fluid. Discriminatory patterns arising from decision-tree-based classification and regression analysis distinguished between serum and follicular fluid with a sensitivity and specificity of 100%. CONCLUSIONS: Quantitative and qualitative differences indicate selective transport processes rather than mere filtration across the blood-follicle barrier. Identified proteins as well as characteristic peptide and/or protein signatures might emerge as potential candidates for diagnostic markers of follicle and/or oocyte maturation and thus oocyte quality. KW - human follicular fluid KW - peptide KW - protein KW - proteome KW - serum Y1 - 2006 U6 - https://doi.org/10.1093/humrep/del257 SN - 0268-1161 VL - 21 IS - 11 SP - 2960 EP - 2968 PB - Univ. Press CY - Oxford ER - TY - JOUR A1 - Schweigert, Florian J. A1 - Steinhagen, Beate A1 - Trüpschuch, Annett A1 - Siemann, A. A1 - Büscher, Ulrich A1 - Dudenhausen, Joachim W. T1 - Transfer of carotenoids, alfa-tocopherol and retinol from plasma into follicular fluid in women Y1 - 2000 ER - TY - BOOK A1 - Schütz, Chr. A1 - Kamtsiuris, P. A1 - Bergmann, K. A1 - Schweigert, Florian J. A1 - Dudenhausen, Joachim W. A1 - Bergmann, R. T1 - Prävalenz und Risikofaktoren von Folsäuremangel bei jungen Müttern in Berlin Y1 - 2001 ER - TY - JOUR A1 - Steinhagen, Beate A1 - Siemann, A. A1 - Büscher, Ulrich A1 - Dudenhausen, Joachim W. A1 - Schweigert, Florian J. T1 - Untersuchungen zum Transfer von Carotinoiden aus dem Plasma in die Follikelflüssigkeit der Frau Y1 - 2000 ER - TY - JOUR A1 - Steinhagen, Beate A1 - Siemann, A. A1 - Büscher, Ulrich A1 - Dudenhausen, Joachim W. A1 - Schweigert, Florian J. T1 - Carotenoids, retinol and tocopherol in plasma and follicular fluid of women Y1 - 2000 ER -