TY - JOUR
A1 - Olimi, Expedito
A1 - Kusstatscher, Peter
A1 - Wicaksono, Wisnu Adi
A1 - Abdelfattah, Ahmed
A1 - Cernava, Tomislav
A1 - Berg, Gabriele
T1 - Insights into the microbiome assembly during different growth stages and storage of strawberry plants
JF - Environmental microbiome
N2 - Background: Microbiome assembly was identified as an important factor for plant growth and health, but this process is largely unknown, especially for the fruit microbiome. Therefore, we analyzed strawberry plants of two cultivars by focusing on microbiome tracking during the different growth stages and storage using amplicon sequencing, qPCR, and microscopic approaches.
Results: Strawberry plants carried a highly diverse microbiome, therein the bacterial families Sphingomonadaceae (25%), Pseudomonadaceae (17%), and Burkholderiaceae (11%); and the fungal family Mycosphaerella (45%) were most abundant. All compartments were colonized by high number of bacteria and fungi (10(7)-10(10) marker gene copies per g fresh weight), and were characterized by high microbial diversity (6049 and 1501 ASVs); both were higher for the belowground samples than in the phyllosphere. Compartment type was the main driver of microbial diversity, structure, and abundance (bacterial: 45%; fungal: 61%) when compared to the cultivar (1.6%; 2.2%). Microbiome assembly was strongly divided for belowground habitats and the phyllosphere; only a low proportion of the microbiome was transferred from soil via the rhizosphere to the phyllosphere. During fruit development, we observed the highest rates of microbial transfer from leaves and flowers to ripe fruits, where most of the bacteria occured inside the pulp. In postharvest fruits, microbial diversity decreased while the overall abundance increased. Developing postharvest decay caused by Botrytis cinerea decreased the diversity as well, and induced a reduction of potentially beneficial taxa.
Conclusion: Our findings provide insights into microbiome assembly in strawberry plants and highlight the importance of microbe transfer during fruit development and storage with potential implications for food health and safety.
KW - Fragaria x ananassa
KW - Microbiome assembly
KW - Fruit pathogens
KW - Bacterial
KW - communities
KW - Fungal communities
KW - Amplicon sequencing
KW - CLSM
Y1 - 2022
U6 - https://doi.org/10.1186/s40793-022-00415-3
SN - 2524-6372
VL - 17
IS - 1
PB - BMC
CY - London
ER -
TY - JOUR
A1 - Liang, Wei
A1 - Heinrich, Ingo
A1 - Helle, Gerhard
A1 - Linan, Isabel Dorado
A1 - Heinken, Thilo
T1 - Applying CLSM to increment core surfaces for histometric analyses a novel advance in quantitative wood anatomy
JF - Dendrochronologia : an interdisciplinary journal of tree-ring science
N2 - A novel procedure has been developed to conduct cell structure measurements on increment core samples of conifers. The procedure combines readily available hardware and software equipment. The essential part of the procedure is the application of a confocal laser scanning microscope (CLSM) which captures images directly from increment cores surfaced with the advanced WSL core-microtome. Cell wall and lumen are displayed with a strong contrast due to the monochrome black and green nature of the images. Consecutive images are merged into long images representing entire increment cores which are then analysed for cell structures in suitable software.
KW - Wood anatomy
KW - Cell structures
KW - Confocal laser scanning microscopy
KW - CLSM
KW - Dendrochronology
KW - Surface preparation
Y1 - 2013
U6 - https://doi.org/10.1016/j.dendro.2012.09.002
SN - 1125-7865
VL - 31
IS - 2
SP - 140
EP - 145
PB - Elsevier
CY - Jena
ER -