TY  - JOUR
A1  - Draude, F.
A1  - Galla, S.
A1  - Pelster, Axel
A1  - Tentschert, J.
A1  - Jungnickel, H.
A1  - Haase, Alfred
A1  - Mantion, Alexandre
A1  - Thuenemann, Andreas F.
A1  - Taubert, Andreas
A1  - Luch, A.
A1  - Arlinghaus, H. F.
T1  - ToF-SIMS and Laser-SNMS analysis of macrophages after exposure to silver nanoparticles
JF  - Surface and interface analysis : an international journal devoted to the development and application of techniques for the analysis surfaces, interfaces and thin films
N2  - Silver nanoparticles (SNPs) are among the most commercialized nanoparticles because of their antibacterial effects. Besides being employed, e. g. as a coatingmaterial for sterile surfaces in household articles and appliances, the particles are also used in a broad range of medical applications. Their antibacterial properties make SNPs especially useful for wound disinfection or as a coating material for prostheses and surgical instruments. Because of their optical characteristics, the particles are of increasing interest in biodetection as well. Despite the widespread use of SNPs, there is little knowledge of their toxicity. Time-of-flight secondary ion mass spectrometry (ToF-SIMS) and laser post-ionization secondary neutral mass spectrometry (Laser-SNMS) were used to investigate the effects of SNPs on human macrophages derived from THP-1 cells in vitro. For this purpose, macrophages were exposed to SNPs. The SNP concentration ranges were chosen with regard to functional impairments of the macrophages. To optimize the analysis of the macrophages, a special silicon wafer sandwich preparation technique was employed; ToF-SIMS was employed to characterize fragments originating from macrophage cell membranes. With the use of this optimized sample preparation method, the SNP-exposed macrophages were analyzed with ToF-SIMS and with Laser-SNMS. With Laser-SNMS, the three-dimensional distribution of SNPs in cells could be readily detected with very high efficiency, sensitivity, and submicron lateral resolution. We found an accumulation of SNPs directly beneath the cell membrane in a nanoparticular state as well as agglomerations of SNPs inside the cells.
KW  - Laser-SNMS
KW  - ToF-SIMS
KW  - life sciences
KW  - imaging
KW  - nanoparticles
KW  - three-dimensional depth profiling
Y1  - 2013
U6  - https://doi.org/10.1002/sia.4902
SN  - 0142-2421
VL  - 45
IS  - 1
SP  - 286
EP  - 289
PB  - Wiley-Blackwell
CY  - Hoboken
ER  - 
TY  - THES
A1  - Grewe-Salfeld, Mirjam
T1  - Biohacking, bodies and do-it-yourself
BT  - the cultural politics of hacking life itself
T2  - American Culture Studies ; 36
N2  - From self-help books and nootropics, to self-tracking and home health tests, to the tinkering with technology and biological particles – biohacking brings biology, medicine, and the material foundation of life into the sphere of »do-it-yourself«. This trend has the potential to fundamentally change people's relationship with their bodies and biology but it also creates new cultural narratives of responsibility, authority, and differentiation. Covering a broad range of examples, this book explores practices and representations of biohacking in popular culture, discussing their ambiguous position between empowerment and requirement, promise and prescription.
N2  - Von Selbsthilfe-Bücher zu "Nootropics", Selbsttracking und Gesundheitstests für den Heimgebrauch, zum experimentieren mit Technologien und biologischen Partikeln - Biohacking vereinigt Biologie, Medizin und die materielle Basis des Lebens mit Praktiken von "do-it-yourself". Dieser Trend birgt das Potential die Beziehung von Menschen mit ihren Körpern und ihrer eigenen Biologie grundlegend zu verändern. Gleichzeitig entstehen dadurch neue kulturelle Narrative von Verantwortung, Autorität, und Differenzierung. Anhand vieler Beispiele untersucht dieses Buch Praktiken und pop-kulturelle Repräsentationen von "biohacking" und beleuchtet ihre mehrdeutige Position zwischen Empowerment und Voraussetzung, Versprechen und Vorschrift.
KW  - culture
KW  - representation
KW  - biology
KW  - medicine
KW  - biocultures
KW  - biohacking
KW  - biotechnology
KW  - cultural narratives
KW  - DIY
KW  - America
KW  - body
KW  - biopolitics
KW  - American studies
KW  - life sciences
KW  - cultural studies
KW  - Amerika
KW  - Amerikastudien
KW  - DIY
KW  - Do-it-yourself
KW  - Biohacking
KW  - Biologie
KW  - Biopolitik
KW  - Biotechnology
KW  - Körper
KW  - Kulturwissenschaft
KW  - Narrative
KW  - Medizin
Y1  - 2020
SN  - 978-3-8376-6004-3
SN  - 978-3-8394-6004-7
PB  - transcript Verlag
CY  - Bielefeld
ER  -