TY  - JOUR
A1  - Meyer, Sören
A1  - Markova, Mariya
A1  - Pohl, Gabriele
A1  - Marschall, Talke Anu
A1  - Pivovarova, Olga
A1  - Pfeiffer, Andreas F. H.
A1  - Schwerdtle, Tanja
T1  - Development, validation and application of an ICP-MS/MS method to quantify minerals and (ultra-)trace elements in human serum
JF  - Journal of trace elements in medicine and biology
N2  - Multi-element determination in human samples is very challenging. Especially in human intervention studies sample volumes are often limited to a few microliters and due to the high number of samples a high-throughput is indispensable. Here, we present a state-of-the-art ICP-MS/MS-based method for the analysis of essential (trace) elements, namely Mg, Ca, Fe, Cu, Zn, Mo, Se and I, as well as food-relevant toxic elements such as As and Cd. The developed method was validated regarding linearity of the calibration curves, method LODs and LOQs, selectivity and trueness as well as precision. The established reliable method was applied to quantify the element serum concentrations of participants of a human intervention study (LeguAN). The participants received isocaloric diets, either rich in plant protein or in animal protein. While the serum concentrations of Mg and Mo increased in participants receiving the plant protein-based diet (above all legumes), the Se concentration in serum decreased. In contrast, the animal protein-based diet, rich in meat and dairy products, resulted in an increased Se concentration in serum.
KW  - ICP-MS
KW  - Elemental blood serum concentration
KW  - Human nutritional intervention
Y1  - 2018
U6  - https://doi.org/10.1016/j.jtemb.2018.05.012
SN  - 0946-672X
VL  - 49
SP  - 157
EP  - 163
PB  - Elsevier GMBH
CY  - München
ER  - 
TY  - JOUR
A1  - Wehe, Christoph A.
A1  - Pieper, Imke
A1  - Holtkamp, Michael
A1  - Thyssen, Georgina M.
A1  - Sperling, Michael
A1  - Schwerdtle, Tanja
A1  - Karst, Uwe
T1  - On-line species-unspecific isotope dilution analysis in the picomolar range reveals the time- and species-depending mercury uptake in human astrocytes
JF  - Analytical & bioanalytical chemistry
N2  - In order to reveal the time-depending mercury species uptake by human astrocytes, a novel approach for total mercury analysis is presented, which uses an accelerated sample introduction system combined on-line with an inductively coupled plasma mass spectrometer equipped with a collision/reaction cell. Human astrocyte samples were incubated with inorganic mercury (HgCl2), methylmercury chloride (MeHgCl), and thimerosal. After 1-h incubation with Hg2+, cellular concentrations of 3 mu M were obtained, whereas for organic species, concentrations of 14-18 mu M could be found. After 24 h, a cellular accumulation factor of 0.3 was observed for the cells incubated with Hg2+, whereas the organic species both showed values of about 5. Due to the obtained steady-state signals, reliable results with relative standard deviations of well below 5 % and limits of detection in the concentration range of 1 ng L-1 were obtained using external calibration and species-unspecific isotope dilution analysis approaches. The results were further validated using atomic fluorescence spectrometry.
KW  - Mercury
KW  - Thimerosal
KW  - Astrocytes
KW  - ICP-MS
KW  - Isotope dilution analysis
KW  - Automation
Y1  - 2014
U6  - https://doi.org/10.1007/s00216-013-7608-4
SN  - 1618-2642
SN  - 1618-2650
VL  - 406
IS  - 7
SP  - 1909
EP  - 1916
PB  - Springer
CY  - Heidelberg
ER  -