TY  - JOUR
A1  - Reinkensmeier, Annika
A1  - Bassler, Sara
A1  - Schlueter, Oliver
A1  - Rohn, Sascha
A1  - Rawel, Harshadrai Manilal
T1  - Characterization of individual proteins in pea protein isolates and air classified samples
JF  - Food research international
N2  - Generally, pea proteins are extracted at comparatively acidic or basic pH values to provide a basis for protein isolate production. Such processing steps result in partial denaturation of the proteins rendering them in most cases insoluble at food processing pH conditions and limiting their application in food products. Here, the comparison of the solubility properties of pea proteins in protein enriched fractions deriving from air classification is reported. Protein content, solubility, and physicochemical parameters of different fractions of the pea (Pisum sativum) variety 'Salamanca' were investigated as a function of pH using SDS-PAGE and surface hydrophobicity. Whole pea flour (20% protein), air classified, protein-enriched pea flour (48% protein), pea flour made from hulls (2.8% protein), and pea protein isolate (81% protein) served as test materials. Fractionation and pH value affected the composition and surface hydrophobicity of the proteins as well as the content of trypsin inhibitors. All samples showed a high buffering capacity in the range of pH 4 to 10. The direct comparison documents the comparatively better protein quality of the air classified, protein enriched pea fraction. The solubility of the pea protein isolate can be improved by using selected additives, giving new possibilities for plant protein application. Relevant technofunctional properties were determined and compared with two commercially available pea-based products (whole pea flour and an isolate). Water binding capacity was highest for the commercially available pea flour followed by the pea hull flour. Fat binding capacity remained more or less unchanged. (C) 2015 Elsevier Ltd. All rights reserved.
KW  - Pea flour
KW  - Pea protein isolate
KW  - Extraction
KW  - Physicochemical properties
KW  - Technofunctional properties
Y1  - 2015
U6  - https://doi.org/10.1016/j.foodres.2015.05.009
SN  - 0963-9969
SN  - 1873-7145
VL  - 76
SP  - 160
EP  - 167
PB  - Elsevier
CY  - Amsterdam
ER  -