TY  - JOUR
A1  - Ribeiro, Dimas M.
A1  - Araujo, Wagner L.
A1  - Fernie, Alisdair R.
A1  - Schippers, Jos H. M.
A1  - Müller-Röber, Bernd
T1  - Translatome and metabolome effects triggered by gibberellins during rosette growth in Arabidopsis
JF  - Journal of experimental botany
N2  - Although gibberellins (GAs) are well known for their growth control function, little is known about their effects on primary metabolism. Here the modulation of gene expression and metabolic adjustment in response to changes in plant (Arabidopsis thaliana) growth imposed on varying the gibberellin regime were evaluated. Polysomal mRNA populations were profiled following treatment of plants with paclobutrazol (PAC), an inhibitor of GA biosynthesis, and gibberellic acid (GA(3)) to monitor translational regulation of mRNAs globally. Gibberellin levels did not affect levels of carbohydrates in plants treated with PAC and/or GA(3). However, the tricarboxylic acid cycle intermediates malate and fumarate, two alternative carbon storage molecules, accumulated upon PAC treatment. Moreover, an increase in nitrate and in the levels of the amino acids was observed in plants grown under a low GA regime. Only minor changes in amino acid levels were detected in plants treated with GA(3) alone, or PAC plus GA(3). Comparison of the molecular changes at the transcript and metabolite levels demonstrated that a low GA level mainly affects growth by uncoupling growth from carbon availability. These observations, together with the translatome changes, reveal an interaction between energy metabolism and GA-mediated control of growth to coordinate cell wall extension, secondary metabolism, and lipid metabolism.
KW  - Gibberellin
KW  - growth
KW  - paclobutrazol
KW  - primary metabolism
KW  - translatome
Y1  - 2012
U6  - https://doi.org/10.1093/jxb/err463
SN  - 0022-0957
VL  - 63
IS  - 7
SP  - 2769
EP  - 2786
PB  - Oxford Univ. Press
CY  - Oxford
ER  -