TY - JOUR A1 - Gurke, Marie A1 - Vidal-Gorosquieta, Amalia A1 - Pajimans, Johanna L. A. A1 - Wȩcek, Karolina A1 - Barlow, Axel A1 - González-Fortes, Gloria M. A1 - Hartmann, Stefanie A1 - Grandal-d’Anglade, Aurora A1 - Hofreiter, Michael T1 - Insight into the introduction of domestic cattle and the process of Neolithization to the Spanish region Galicia by genetic evidence JF - PLoS ONE N2 - Domestic cattle were brought to Spain by early settlers and agricultural societies. Due to missing Neolithic sites in the Spanish region of Galicia, very little is known about this process in this region. We sampled 18 cattle subfossils from different ages and different mountain caves in Galicia, of which 11 were subject to sequencing of the mitochondrial genome and phylogenetic analysis, to provide insight into the introduction of cattle to this region. We detected high similarity between samples from different time periods and were able to compare the time frame of the first domesticated cattle in Galicia to data from the connecting region of Cantabria to show a plausible connection between the Neolithization of these two regions. Our data shows a close relationship of the early domesticated cattle of Galicia and modern cow breeds and gives a general insight into cattle phylogeny. We conclude that settlers migrated to this region of Spain from Europe and introduced common European breeds to Galicia. KW - Haplogroups KW - Mitochondria KW - Cattle KW - Genomics KW - Domestic animals KW - Livestock KW - Single nucleotide polymorphisms KW - Neolithic period Y1 - 2020 U6 - https://doi.org/10.1371/journal.pone.0249537 SN - 1932-6203 VL - 16 IS - 4 PB - Public Library of Science CY - San Francisco ER - TY - JOUR A1 - Apriyanto, Ardha A1 - Tambunan, Van Basten T1 - Draft genome sequence, annotation, and SSR mining data of Elaeidobius kamerunicus Faust., an essential oil palm pollinating weevil JF - Data in Brief N2 - Elaeidobius kamerunicus Faust. (Coleoptera: Curculionidae) is an essential insect pollinator in oil palm plantations. Recently, researches have been undertaken to improve pollination efficiency using this species. A fundamental understanding of the genes related to this pollinator behavior is necessary to achieve this goal. Here, we present the draft genome sequence, annotation, and simple sequence repeat (SSR) marker data for this pollinator. In total, 34.97 Gb of sequence data from one male individual (monoisolate) were obtained using Illumina short-read platform NextSeq 500. The draft genome assembly was found to be 269.79 Mb and about 59.9% of completeness based on Benchmarking Universal Single-Copy Orthologs (BUSCO) assessment. Functional gene annotation predicted about 26.566 genes. Also, a total of 281.668 putative SSR markers were identified. This draft genome sequence is a valuable resource for understanding the population genetics, phylogenetics, dispersal patterns, and behavior of this species. KW - Whole-genome sequencing KW - NGS KW - Simple Sequence Repeat KW - Weevil KW - Curculionidae KW - Oil Palm KW - Pollinator KW - Genomics Y1 - 2021 U6 - https://doi.org/10.1016/j.dib.2021.106745 SN - 2352-3409 VL - 34 PB - Elsevier CY - Amsterdam ER - TY - JOUR A1 - Paijmans, Johanna L. A. A1 - Barlow, Axel A1 - Henneberger, Kirstin A1 - Fickel, Jörns A1 - Hofreiter, Michael A1 - Foerste, Daniel W. G. T1 - Ancestral mitogenome capture of the Southeast Asian banded linsang JF - PLoS ONE N2 - Utilising a reconstructed ancestral mitochondrial genome of a clade to design hybridisation capture baits can provide the opportunity for recovering mitochondrial sequences from all its descendent and even sister lineages. This approach is useful for taxa with no extant close relatives, as is often the case for rare or extinct species, and is a viable approach for the analysis of historical museum specimens. Asiatic linsangs (genus Prionodon) exemplify this situation, being rare Southeast Asian carnivores for which little molecular data is available. Using ancestral capture we recover partial mitochondrial genome sequences for seven banded linsangs (P. linsang) from historical specimens, representing the first intraspecific genetic dataset for this species. We additionally assemble a high quality mitogenome for the banded linsang using shotgun sequencing for time-calibrated phylogenetic analysis. This reveals a deep divergence between the two Asiatic linsang species (P. linsang, P. pardicolor), with an estimated divergence of ~12 million years (Ma). Although our sample size precludes any robust interpretation of the population structure of the banded linsang, we recover two distinct matrilines with an estimated tMRCA of ~1 Ma. Our results can be used as a basis for further investigation of the Asiatic linsangs, and further demonstrate the utility of ancestral capture for studying divergent taxa without close relatives. KW - Shotgun sequencing KW - Mitochondria KW - Phylogenetics KW - Phylogenetic analysis KW - Paleogenetics KW - Sequence alignment KW - Genomics KW - Museum collections Y1 - 2019 U6 - https://doi.org/10.1371/journal.pone.0234385 SN - 1932-6203 VL - 15 IS - 6 PB - PLOS CY - San Francisco, California, US ER -