TY  - JOUR
A1  - Bäurle, Isabel
A1  - Brzezinka, Krzysztof
A1  - Altmann, Simone
T1  - BRUSHY1/TONSOKU/MGOUN3 is required for heat stress memory
JF  - Plant Cell & Environment
N2  - Plants encounter biotic and abiotic stresses many times during their life cycle and this limits their productivity. Moderate heat stress (HS) primes a plant to survive higher temperatures that are lethal in the naïve state. Once temperature stress subsides, the memory of the priming event is actively retained for several days preparing the plant to better cope with recurring HS. Recently, chromatin regulation at different levels has been implicated in HS memory. Here, we report that the chromatin protein BRUSHY1 (BRU1)/TONSOKU/MGOUN3 plays a role in the HS memory in Arabidopsis thaliana. BRU1 is also involved in transcriptional gene silencing and DNA damage repair. This corresponds with the functions of its mammalian orthologue TONSOKU‐LIKE/NFΚBIL2. During HS memory, BRU1 is required to maintain sustained induction of HS memory‐associated genes, whereas it is dispensable for the acquisition of thermotolerance. In summary, we report that BRU1 is required for HS memory in A. thaliana, and propose a model where BRU1 mediates the epigenetic inheritance of chromatin states across DNA replication and cell division.
KW  - Arabidopsis thaliana
KW  - BRUSHY1
KW  - chromatin
KW  - priming
Y1  - 2019
U6  - https://doi.org/10.1111/pce.13365
VL  - 42
SP  - 771
EP  - 781
ER  - 
TY  - JOUR
A1  - Lämke, Jörn
A1  - Brzezinka, Krzysztof
A1  - Altmann, Simone
A1  - Bäurle, Isabel
T1  - A hit-and-run heat shock factor governs sustained histone methylation and transcriptional stress memory
JF  - The EMBO journal
N2  - In nature, plants often encounter chronic or recurring stressful conditions. Recent results indicate that plants can remember a past exposure to stress to be better prepared for a future stress incident. However, the molecular basis of this is poorly understood. Here, we report the involvement of chromatin modifications in the maintenance of acquired thermotolerance (heat stress [HS] memory). HS memory is associated with the accumulation of histone H3 lysine 4 di- and trimethylation at memory-related loci. This accumulation outlasts their transcriptional activity and marks them as recently transcriptionally active. High accumulation of H3K4 methylation is associated with hyper-induction of gene expression upon a recurring HS. This transcriptional memory and the sustained accumulation of H3K4 methylation depend on HSFA2, a transcription factor that is required for HS memory, but not initial heat responses. Interestingly, HSFA2 associates with memory-related loci transiently during the early stages following HS. In summary, we show that transcriptional memory after HS is associated with sustained H3K4 hyper-methylation and depends on a hit-and-run transcription factor, thus providing a molecular framework for HS memory.
KW  - chromatin
KW  - H3K4 methylation
KW  - heat shock transcription factor
KW  - priming
KW  - transcriptional memory
Y1  - 2016
U6  - https://doi.org/10.15252/embj.201592593
SN  - 0261-4189
SN  - 1460-2075
VL  - 35
SP  - 162
EP  - 175
PB  - Wiley-Blackwell
CY  - Hoboken
ER  -