TY  - JOUR
A1  - Dong, Yanni
A1  - Gupta, Saurabh
A1  - Sievers, Rixta
A1  - Wargent, Jason J.
A1  - Wheeler, David
A1  - Putterill, Joanna
A1  - Macknight, Richard
A1  - Gechev, Tsanko S.
A1  - Müller-Röber, Bernd
A1  - Dijkwel, Paul P.
T1  - Genome draft of the Arabidopsis relative Pachycladon cheesemanii reveals environment
JF  - BMC genomics
N2  - BackgroundPachycladon cheesemanii is a close relative of Arabidopsis thaliana and is an allotetraploid perennial herb which is widespread in the South Island of New Zealand. It grows at altitudes of up to 1000m where it is subject to relatively high levels of ultraviolet (UV)-B radiation. To gain first insights into how Pachycladon copes with UV-B stress, we sequenced its genome and compared the UV-B tolerance of two Pachycladon accessions with those of two A. thaliana accessions from different altitudes.ResultsA high-quality draft genome of P. cheesemanii was assembled with a high percentage of conserved single-copy plant orthologs. Synteny analysis with genomes from other species of the Brassicaceae family found a close phylogenetic relationship of P. cheesemanii with Boechera stricta from Brassicaceae lineage I. While UV-B radiation caused a greater growth reduction in the A. thaliana accessions than in the P. cheesemanii accessions, growth was not reduced in one P. cheesemanii accession. The homologues of A. thaliana UV-B radiation response genes were duplicated in P. cheesemanii, and an expression analysis of those genes indicated that the tolerance mechanism in P. cheesemanii appears to differ from that in A. thaliana.ConclusionAlthough the P. cheesemanii genome shows close similarity with that of A. thaliana, it appears to have evolved novel strategies allowing the plant to tolerate relatively high UV-B radiation.
KW  - Abiotic stress
KW  - Arabidopsis
KW  - Genome assembly
KW  - Pachycladon
KW  - UV-B tolerance
Y1  - 2019
U6  - https://doi.org/10.1186/s12864-019-6084-4
SN  - 1471-2164
VL  - 20
IS  - 1
PB  - BMC
CY  - London
ER  -