TY  - JOUR
A1  - Federico, Stefania
A1  - Pierce, Benjamin F.
A1  - Piluso, Susanna
A1  - Wischke, Christian
A1  - Lendlein, Andreas
A1  - Neffe, Axel T.
T1  - Design of Decorin-Based Peptides That Bind to CollagenI and their Potential as Adhesion Moieties in Biomaterials
JF  - Angewandte Chemie : a journal of the Gesellschaft Deutscher Chemiker ; International edition
N2  - Mimicking the binding epitopes of protein-protein interactions by using small peptides is important for generating modular biomimetic systems. A strategy is described for the design of such bioactive peptides without accessible structural data for the targeted interaction, and the effect of incorporating such adhesion peptides in complex biomaterial systems is demonstrated. The highly repetitive structure of decorin was analyzed to identify peptides that are representative of the inner and outer surface, and it was shown that only peptides based on the inner surface of decorin bind to collagen. The peptide with the highest binding affinity for collagenI, LHERHLNNN, served to slow down the diffusion of a conjugated dye in a collagen gel, while its dimer could physically crosslink collagen, thereby enhancing the elastic modulus of the gel by one order of magnitude. These results show the potential of the identified peptides for the design of biomaterials for applications in regenerative medicine.
KW  - biomaterials
KW  - collagen
KW  - gels
KW  - peptides
KW  - protein-protein interactions
Y1  - 2015
U6  - https://doi.org/10.1002/anie.201505227
SN  - 1433-7851
SN  - 1521-3773
VL  - 54
IS  - 37
SP  - 10980
EP  - 10984
PB  - Wiley-VCH
CY  - Weinheim
ER  - 
TY  - JOUR
A1  - Ast, Sandra
A1  - Schwarze, Thomas
A1  - Müller, Holger
A1  - Sukhanov, Aleksey
A1  - Michaelis, Stefanie
A1  - Wegener, Joachim
A1  - Wolfbeis, Otto S.
A1  - Körzdörfer, Thomas
A1  - Dürkop, Axel
A1  - Holdt, Hans-Jürgen
T1  - A highly K+-Selective Phenylaza-[18]crown-6-Lariat-Ether-Based Fluoroionophore and its application in the sensing of K+ Ions with an optical sensor film and in cells
JF  - Chemistry - a European journal
N2  - Herein, we report the synthesis of two phenylaza-[18]crown-6 lariat ethers with a coumarin fluorophore (1 and 2) and we reveal that compound 1 is an excellent probe for K+ ions under simulated physiological conditions. The presence of a 2-methoxyethoxy lariat group at the ortho position of the anilino moiety is crucial to the substantially increased stability of compounds 1 and 2 over their lariat-free phenylaza-[18] crown-6 ether analogues. Probe 1 shows a high K+/Na+ selectivity and a 2.5-fold fluorescence enhancement was observed in the presence of 100 mm K+ ions. A fluorescent membrane sensor, which was prepared by incorporating probe 1 into a hydrogel, showed a fully reversible response, a response time of 150 s, and a signal change of 7.8% per 1 mm K+ within the range 1-10 mm K+. The membrane was easily fabricated (only a single sensing layer on a solid polyester support), yet no leaching was observed. Moreover, compound 1 rapidly permeated into cells, was cytocompatible, and was suitable for the fluorescent imaging of K+ ions on both the extracellular and intracellular levels.
KW  - crown compounds
KW  - fluorescence
KW  - gels
KW  - potassium
KW  - sensors
Y1  - 2013
U6  - https://doi.org/10.1002/chem.201302350
SN  - 0947-6539
SN  - 1521-3765
VL  - 19
IS  - 44
SP  - 14911
EP  - 14917
PB  - Wiley-VCH
CY  - Weinheim
ER  -