TY  - JOUR
A1  - Schorn, Sina
A1  - Salman-Carvalho, Verena
A1  - Littmann, Sten
A1  - Ionescu, Danny
A1  - Grossart, Hans-Peter
A1  - Cypionka, Heribert
T1  - Cell architecture of the giant sulfur bacterium achromatium oxaliferum
BT  - Extra-cytoplasmic localization of calcium carbonate bodies
JF  - FEMS Microbiology Ecology
N2  - Achromatium oxaliferum is a large sulfur bacterium easily recognized by large intracellular calcium carbonate bodies. Although these bodies often fill major parts of the cells' volume, their role and specific intracellular location are unclear. In this study, we used various microscopy and staining techniques to identify the cell compartment harboring the calcium carbonate bodies. We observed that Achromatium cells often lost their calcium carbonate bodies, either naturally or induced by treatments with diluted acids, ethanol, sodium bicarbonate and UV radiation which did not visibly affect the overall shape and motility of the cells (except for UV radiation). The water-soluble fluorescent dye fluorescein easily diffused into empty cavities remaining after calcium carbonate loss. Membranes (stained with Nile Red) formed a network stretching throughout the cell and surrounding empty or filled calcium carbonate cavities. The cytoplasm (stained with FITC and SYBR Green for nucleic acids) appeared highly condensed and showed spots of dissolved Ca2+ (stained with Fura-2). From our observations, we conclude that the calcium carbonate bodies are located in the periplasm, in extra-cytoplasmic pockets of the cytoplasmic membrane and are thus kept separate from the cell's cytoplasm. This periplasmic localization of the carbonate bodies might explain their dynamic formation and release upon environmental changes.
KW  - sulfur-bacteria
KW  - calcium carbonate inclusions
KW  - extra-cytoplasmic pockets
KW  - calcite
Y1  - 2019
U6  - https://doi.org/10.1093/femsec/fiz200
SN  - 1574-6941
VL  - 96
IS  - 2
SP  - 1
EP  - 8
PB  - Oxford University Press
CY  - Oxford
ER  - 
TY  - JOUR
A1  - Wiebke, Ullmann
T1  - Warum hat Bayern mehr Feldhasen als Brandenburg?
JF  - Vielfalt in der Uckermark : Forschungsprojekte 2015 - 2018
Y1  - 2019
SP  - 46
EP  - 47
PB  - oerding print GmbH
CY  - Braunschweig
ER  - 
TY  - JOUR
A1  - Teckentrup, Lisa
T1  - Gefahr an jeder Ecke
BT  - wie die Landschaftsstruktur die Verteilung von Beutetieren beeinflusst
JF  - Vielfalt in der Uckermark : Forschungsprojekte 2015 - 2018
Y1  - 2019
SP  - 54
EP  - 55
PB  - oerding print GmbH
CY  - Braunschweig
ER  - 
TY  - JOUR
A1  - Schäfer, Merlin
T1  - Mut macht einsam
BT  - der Einfluss von Persönlichkeit auf das Zusammenleben von Tieren
JF  - Vielfalt in der Uckermark : Forschungsprojekte 2015 - 2018
Y1  - 2019
SP  - 52
EP  - 53
PB  - oerding print GmbH
CY  - Braunschweig
ER  - 
TY  - JOUR
A1  - Kowalski, Gabriele Joanna
T1  - Auf dem Sprung
BT  - wie bewegen sich Tiere durch die Landschaft?
JF  - Vielfalt in der Uckermark : Forschungsprojekte 2015 - 2018
Y1  - 2019
SP  - 41
EP  - 42
PB  - oerding print GmbH
CY  - Braunschweig
ER  - 
TY  - JOUR
A1  - Raatz, Larissa
T1  - Wirtschaften in einer reich strukturierten Landschaft - geht das ?
JF  - Vielfalt in der Uckermark : Forschungsprojekte 2015 - 2018
Y1  - 2019
SP  - 32
EP  - 33
PB  - oerding print GmbH
CY  - Braunschweig
ER  - 
TY  - JOUR
A1  - Lozada Gobilard, Sissi Donna
T1  - Können auch Pflanzen zwischen den Söllen "wandern"
JF  - Vielfalt in der Uckermark : Forschungsprojekte 2015 - 2018
Y1  - 2019
SP  - 30
EP  - 31
PB  - oerding print GmbH
CY  - Braunschweig
ER  - 
TY  - JOUR
A1  - Litwin, Magdalena
A1  - Colangeli, Pierluigi
T1  - Wie und wohin reisen Wasserflöhe?
JF  - Vielfalt in der Uckermark : Forschungsprojekte 2015 - 2018
Y1  - 2019
SP  - 28
EP  - 29
PB  - oerding print GmbH
CY  - Braunschweig
ER  - 
TY  - JOUR
A1  - Maaß, Stefanie
T1  - Blick in die Zukunft
BT  - wie werden sich Pflanzengemeinschaften in Brandenburg verändern?
JF  - Vielfalt in der Uckermark : Forschungsprojekte 2015 - 2018
Y1  - 2019
SP  - 24
EP  - 25
PB  - oerding print GmbH
CY  - Braunschweig
ER  - 
TY  - JOUR
A1  - Weiß, Lina
A1  - Wulff, Monika
T1  - Veränderung der Landnutzung in der nord-westlichen Uckermark von 1780 bis heute
JF  - Vielfalt in der Uckermark : Forschungsprojekte 2015 - 2018
Y1  - 2019
SP  - 20
EP  - 21
PB  - oerding print GmbH
CY  - Braunschweig
ER  -