TY - JOUR A1 - Schweigel, Ulrike A1 - Batsios, Petros A1 - Müller-Taubenberger, Annette A1 - Gräf, Ralph A1 - Grafe, Marianne T1 - Dictyostelium spastin is involved in nuclear envelope dynamics during semi-closed mitosis JF - Nucleus N2 - Dictyostelium amoebae perform a semi-closed mitosis, in which the nuclear envelope is fenestrated at the insertion sites of the mitotic centrosomes and around the central spindle during karyokinesis. During late telophase the centrosome relocates to the cytoplasmic side of the nucleus, the central spindle disassembles and the nuclear fenestrae become closed. Our data indicate that Dictyostelium spastin (DdSpastin) is a microtubule-binding and severing type I membrane protein that plays a role in this process. Its mitotic localization is in agreement with a requirement for the removal of microtubules that would hinder closure of the fenestrae. Furthermore, DdSpastin interacts with the HeH/ LEM-family protein Src1 in BioID analyses as well as the inner nuclear membrane protein Sun1, and shows subcellular co-localizations with Src1, Sun1, the ESCRT component CHMP7 and the IST1-like protein filactin, suggesting that the principal pathway of mitotic nuclear envelope remodeling is conserved between animals and Dictyostelium amoebae. KW - Spastin KW - LEM-domain KW - ESCRT KW - sun1 KW - dictyostelium KW - nuclear envelope KW - mitosis Y1 - 2022 U6 - https://doi.org/10.1080/19491034.2022.2047289 SN - 1949-1034 SN - 1949-1042 VL - 13 IS - 1 SP - 144 EP - 154 PB - Taylor & Francis Group CY - Philadelphia ER -