TY - JOUR A1 - Zurnic, Irena A1 - Hütter, Sylvia A1 - Rzeha, Ute A1 - Stanke, Nicole A1 - Reh, Juliane A1 - Müllers, Erik A1 - Hamann, Martin V. A1 - Kern, Tobias A1 - Gerresheim, Gesche K. A1 - Lindel, Fabian A1 - Serrao, Erik A1 - Lesbats, Paul A1 - Engelman, Alan N. A1 - Cherepanov, Peter A1 - Lindemann, Dirk T1 - Interactions of Prototype Foamy Virus Capsids with Host Cell Polo-Like Kinases Are Important for Efficient Viral DNA Integration JF - PLoS Pathogens N2 - Unlike for other retroviruses, only a few host cell factors that aid the replication of foamy viruses (FVs) via interaction with viral structural components are known. Using a yeast-two-hybrid (Y2H) screen with prototype FV (PFV) Gag protein as bait we identified human polo-like kinase 2 (hPLK2), a member of cell cycle regulatory kinases, as a new interactor of PFV capsids. Further Y2H studies confirmed interaction of PFV Gag with several PLKs of both human and rat origin. A consensus Ser-Thr/Ser-Pro (S-T/S-P) motif in Gag, which is conserved among primate FVs and phosphorylated in PFV virions, was essential for recognition by PLKs. In the case of rat PLK2, functional kinase and polo-box domains were required for interaction with PFV Gag. Fluorescently-tagged PFV Gag, through its chromatin tethering function, selectively relocalized ectopically expressed eGFP-tagged PLK proteins to mitotic chromosomes in a Gag STP motif-dependent manner, confirming a specific and dominant nature of the Gag-PLK interaction in mammalian cells. The functional relevance of the Gag-PLK interaction was examined in the context of replication-competent FVs and single-round PFV vectors. Although STP motif mutated viruses displayed wild type (wt) particle release, RNA packaging and intra-particle reverse transcription, their replication capacity was decreased 3-fold in single-cycle infections, and up to 20-fold in spreading infections over an extended time period. Strikingly similar defects were observed when cells infected with single-round wt Gag PFV vectors were treated with a pan PLK inhibitor. Analysis of entry kinetics of the mutant viruses indicated a post-fusion defect resulting in delayed and reduced integration, which was accompanied with an enhanced preference to integrate into heterochromatin. We conclude that interaction between PFV Gag and cellular PLK proteins is important for early replication steps of PFV within host cells. Y1 - 2016 U6 - https://doi.org/10.1371/journal.ppat.1005860 SN - 1553-7366 SN - 1553-7374 VL - 12 PB - PLoS CY - San Fransisco ER - TY - JOUR A1 - Tscheuschner, Georg A1 - Kaiser, Melanie N. A1 - Lisec, Jan A1 - Beslic, Denis A1 - Muth, Thilo A1 - Krüger, Maren A1 - Mages, Hans Werner A1 - Dorner, Brigitte G. A1 - Knospe, Julia A1 - Schenk, Jörg A. A1 - Sellrie, Frank A1 - Weller, Michael G. T1 - MALDI-TOF-MS-based identification of monoclonal murine Anti-SARS-CoV-2 antibodies within one hour JF - Antibodies N2 - During the SARS-CoV-2 pandemic, many virus-binding monoclonal antibodies have been developed for clinical and diagnostic purposes. This underlines the importance of antibodies as universal bioanalytical reagents. However, little attention is given to the reproducibility crisis that scientific studies are still facing to date. In a recent study, not even half of all research antibodies mentioned in publications could be identified at all. This should spark more efforts in the search for practical solutions for the traceability of antibodies. For this purpose, we used 35 monoclonal antibodies against SARS-CoV-2 to demonstrate how sequence-independent antibody identification can be achieved by simple means applied to the protein. First, we examined the intact and light chain masses of the antibodies relative to the reference material NIST-mAb 8671. Already half of the antibodies could be identified based solely on these two parameters. In addition, we developed two complementary peptide mass fingerprinting methods with MALDI-TOF-MS that can be performed in 60 min and had a combined sequence coverage of over 80%. One method is based on the partial acidic hydrolysis of the protein by 5 mM of sulfuric acid at 99 degrees C. Furthermore, we established a fast way for a tryptic digest without an alkylation step. We were able to show that the distinction of clones is possible simply by a brief visual comparison of the mass spectra. In this work, two clones originating from the same immunization gave the same fingerprints. Later, a hybridoma sequencing confirmed the sequence identity of these sister clones. In order to automate the spectral comparison for larger libraries of antibodies, we developed the online software ABID 2.0. This open-source software determines the number of matching peptides in the fingerprint spectra. We propose that publications and other documents critically relying on monoclonal antibodies with unknown amino acid sequences should include at least one antibody fingerprint. By fingerprinting an antibody in question, its identity can be confirmed by comparison with a library spectrum at any time and context. KW - SARS-CoV-2 antibody KW - reproducibility crisis KW - peptide mass KW - fingerprinting KW - monoclonal antibody KW - traceability KW - identity KW - antibody KW - identification KW - antibody light chain KW - MALDI-TOF-MS Y1 - 2022 U6 - https://doi.org/10.3390/antib11020027 SN - 2073-4468 VL - 11 IS - 2 PB - MDPI CY - Basel ER - TY - JOUR A1 - Stefanakis, Nikolaos A1 - Abel, Markus A1 - Bergner, Andre T1 - Sound Synthesis Based on Ordinary Differential Equations JF - Computer music journal N2 - Ordinary differential equations (ODEs) have been studied for centuries as a means to model complex dynamical processes from the real world. Nevertheless, their application to sound synthesis has not yet been fully exploited. In this article we present a systematic approach to sound synthesis based on first-order complex and real ODEs. Using simple time-dependent and nonlinear terms, we illustrate the mapping between ODE coefficients and physically meaningful control parameters such as pitch, pitch bend, decay rate, and attack time. We reveal the connection between nonlinear coupling terms and frequency modulation, and we discuss the implications of this scheme in connection with nonlinear synthesis. The ability to excite a first-order complex ODE with an external input signal is also examined; stochastic or impulsive signals that are physically or synthetically produced can be presented as input to the system, offering additional synthesis possibilities, such as those found in excitation/filter synthesis and filter-based modal synthesis. Y1 - 2015 U6 - https://doi.org/10.1162/COMJ_a_00314 SN - 0148-9267 SN - 1531-5169 VL - 39 IS - 3 SP - 46 EP - 58 PB - MIT Press CY - Cambridge ER - TY - JOUR A1 - Sass, Enrico T1 - Erfolgreiche Personalauswahl im Technologietransfer BT - der Ablauf eines Bewerbungsgespräches JF - Erkenntnisse zum Wissens- und Technologietransfer N2 - Entscheidungsträger in Forschungseinrichtungen werden oftmals mit der Auswahl von Mitarbeitern im Technologietransfer konfrontiert. Mitarbeiter im Technologietransfer sind wichtige Wissenschaftsmanager, die den anwendungsorientierten Transfer von Forschungsergebnissen und forschungsbasiertem Know-how unterstützen. Zu ihnen zählen Mitarbeiter in den Bereichen Auftragsforschung, Patent- und Gründungsmanagement und Mitarbeiter in transferorientierten Drittmittelprojekten. Für eine professionelle Personalauswahl qualifizierter Technologietransfermitarbeiter ist ein systematisches Bewerbungsgespräch grundlegend. Der vorliegende Artikel gibt einen Einblick in die Struktur und in die einzelnen Phasen eines Bewerbungsgespräches. Dabei wird die Grundstruktur des erfolgreich etablierten Multimodalen Interviews (MMI®) aufgegriffen. Es werden acht verschiedene Interviewphasen erläutert und mit Beispielfragen zur Beurteilung des Bewerbers ergänzt. KW - Personalauswahl KW - Wissenschaftsmanagement KW - Einstellungsinterviews KW - Technologietransfer KW - Bewerbungsgespräche KW - Technologiescouts Y1 - 2013 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-66631 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - JOUR A1 - Sass, Enrico T1 - Academic entrepreneurship BT - Why do university scientists play the entrepreneurship game? JF - Erkenntnisse zum Wissens- und Technologietransfer N2 - Research on entrepreneurial motivation of university scientists is often determined by quantitative methods without taking into account context-related influences. According to different studies, entrepreneurial scientists found a spin-off company due to motives like independency, market opportunity, money or risk of unemployment (short-term contracts). To give a comprehensive explanation, it is important to use a qualitative research view that considers academic rank, norms and values of university scientists. The author spoke with 35 natural scientists and asked professors and research fellows for their entrepreneurial motivation. The results of this study are used to develop a typology of entrepreneurial and non-entrepreneurial scientists within German universities. This paper presents the key findings of the study (Sass 2011). KW - entrepreneurial scientists KW - academic entrepreneurship KW - entrepreneurial motivation KW - entrepreneurial types KW - university spin-offs Y1 - 2013 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-66873 ER - TY - JOUR A1 - Sass, Enrico T1 - Neugierde und Wettkampfsport BT - die Gründungsmotivation von Professoren JF - Erkenntnisse zum Wissens- und Technologietransfer N2 - Ausgründungen aus der Wissenschaft (spin-offs) gehören zu den anspruchsvollsten Instrumenten des Wissens- und Technologietransfers. Die Initiatoren erfolgreicher Gründungsvorhaben sind oftmals engagierte Hochschullehrer, die nicht nur Anerkennung in der Scientific Community suchen, sondern ihre Forschungsergebnisse ebenso in anwendungsorientierte Produkte und Dienstleistungen überführen. Was treibt diese Wissenschaftler an? In welchem Zusammenhang steht die Gründungsmotivation mit der ursprünglichen wissenschaftlichen Motivation? Ist das Initiieren einer Ausgründung mehr als das Lösen eines herausfordernden Rätsels? Der vorliegende Artikel gibt eine Antwort auf diese Fragen und gewährt einen Einblick in die Gründungsmotivation von Hochschulprofessoren aus den Naturwissenschaften. Mit Hilfe einer qualitativen Untersuchung werden verschiedene Gründertypen gebildet. KW - Gründungsmotivation KW - Gründungsmotive KW - Spin-off-Gründer KW - academic entrepreneurship Y1 - 2013 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-66159 PB - Universitätsverlag Potsdam CY - Potsdam ER - TY - THES A1 - Reisswig, Katja T1 - Die „unternehmerische Mission“ von Universitäten : eine neoinstitutionalistische Betrachtung des Aufgabenbereichs Wissens- und Technologietransfer (WTT) an deutschen Hochschulen T1 - The "entrepreneurial mission" of universities : a neoinstitutional view regarding the task of knowledge and technology transfer at universities N2 - Die Arbeit thematisiert die Veränderungen im deutschen Wissenschafts- und Hochschulsystem. Im Mittelpunkt steht die "unternehmerische Mission" von Universitäten. Der Blick wird auf das Aufgabenfeld Wissens- und Technologietransfer (WTT) gerichtet. Anhand dessen werden die Veränderungen, die innerhalb des deutschen Universitätssystems in den vergangenen Jahren erfolgten, nachgezeichnet. Die Erwartungshaltungen an Universitäten haben sich verändert. Ökonomische Sichtweisen nehmen einen immer größeren Stellenwert ein. Die Arbeit baut auf den Prämissen der neoinstitutionalistischen Organisationstheorie auf. Anhand dieser wird gezeigt, wie Erwartungen externer Stakeholder Eingang in Hochschulen finden und sich auf ihre organisatorische Ausgestaltung auswirken. Der Arbeit liegt ein exploratives, qualitatives Untersuchungsdesign zugrunde. In einer Fallstudie werden zwei Universitäten als Fallbeispiele untersucht. Die Untersuchung liefert Antworten auf die Fragen, wie der WTT als Aufgabenbereich an deutschen Universitäten umgesetzt wird, welche Strukturen sich herausgebildet haben und inwieweit eine Institutionalisierung des WTTs an Universitäten erfolgt ist. In der Arbeit werden verschiedene Erhebungsinstrumente im Rahmen einer Triangulation genutzt. Experteninterviews bilden das Hauptanalyseinstrument. Ziel der Untersuchung ist neben der Beantwortung der Forschungsfragen, Hypothesen zu bilden, die für weiterführende Untersuchungen genutzt werden können. Darüber hinaus werden Handlungsempfehlungen für die Umsetzung des WTTs an deutschen Hochschulen gegeben. Die Arbeit richtet sich sowohl an Wissenschaftler als auch Praktiker aus dem Bereich Wissens- und Technologietransfer. N2 - German universities are confronted with ongoing changes. One of these changes encompasses a paradigm shift, which affects the university and research system as a whole. This paradigm shift is the so called "third mission" - or "entrepreneurial mission" - of universities. Universities are asked to make contributions to the economic system via transfer of knowledge and technology. Economic perspectives have an increasing influence on universities and the research system. For this reason, the expectations of external stakeholers are growing. The dissertation shows how these expectations affect the organizational shape of universities. By using neoinstitutional organizational theory, this work finds answers to the following questions: How do universities integrate the "entrepreneurial mission" in their organizational structures? Which organizational structures have emerged? How far has an institutional process been taken place? An explorative, qualitative research design is used and a case study is performed, which includes two universities as examples. Two dimensions were investigated: The structures of universities in the field of technology and knowledge transfer, and the central actors of universities. The main research instrument consists of interviews with experts in the field. Apart from answering the questions posed above, the central aim of the survey is to get a deeper understanding of how German Universities fulfill their “entrepreneurial mission”. Several hypotheses are generated that can be used for further investigations. Furthermore, some practical input is provided for implementing knowledge and technology transfer at German universities. The target audience of this thesis are researchers and practitioners in the field of knowledge and technology transfer. KW - Unternehmerische Universitäten KW - Wissens- und Technologietransfer KW - unternehmerische Mission KW - neoinstitutionale Organisationstheorie KW - entrepreneurial university KW - knowledge- and technology transfer KW - entrepreneurial mission KW - neoinstitutional organizational theory Y1 - 2013 U6 - http://nbn-resolving.de/urn/resolver.pl?urn:nbn:de:kobv:517-opus-70574 ER - TY - JOUR A1 - Mueller-Seitz, Gordon A1 - Reger, Guido T1 - Is open source software living up to its promises? : Insights for open innovation management from two open source software-inspired projects N2 - At present, several virtual initiatives claim to be acting according to the open source software (OSS) arena, which is often deemed a role model for open innovation. Against this background, this research focuses on a comparative case study of two non-profit project networks that attempt to operate in line with the OSS phenomenon: Wikipedia, the online encyclopedia, and the development of an automobile, Open Source car. We show that many parallels to the OSS arena can be drawn in both cases. However, this analysis must be performed cautiously, as several factors limit the applicability of OSS principles to non-software-related arenas. We conclude with a discussion of implications for open innovation research and managerial practice. Y1 - 2009 UR - http://www3.interscience.wiley.com/cgi-bin/issn?DESCRIPTOR=PRINTISSN&VALUE=0033-6807 U6 - https://doi.org/10.1111/j.1467-9310.2009.00565.x SN - 0033-6807 ER - TY - JOUR A1 - Lontsi, Agostiny Marrios A1 - Ohrnberger, Matthias A1 - Krüger, Frank A1 - Sánchez-Sesma, Francisco José T1 - Combining surface-wave phase-velocity dispersion curves and full microtremor horizontal-to-vertical spectral ratio for subsurface sedimentary site characterization JF - Interpretation : a journal of subsurface characterization N2 - We compute seismic velocity profiles by a combined inversion of surface-wave phase-velocity dispersion curves together with the full spectrum of the microtremor horizontal-to-vertical (H/V) spectral ratio at two sediment-covered sites in Germany. The sediment deposits are approximately 100 m thick at the first test site and approximately 400 m thick at the second test site. We have used an extended physical model based on the diffuse wavefield assumption for the interpretation of the observed microtremor H/V spectral ratio. The extension includes the interpretation of the microtremor H/V spectral ratio observed at depth (in boreholes). This full-wavefield approach accounts for the energy contribution from the body and surface waves, and thus it allows for inverting the properties of the shallow subsurface. We have obtained the multimode phase velocity dispersion curves from an independent study, and a description of the extracted branches and their interpretation was developed. The inversion results indicate that the combined approach using seismic ambient noise and actively generated surface-wave data will improve the accuracy of the reconstructed near-surface velocity model, a key step in microzonation, geotechnical engineering, seismic statics corrections, and reservoir imaging. Y1 - 2016 U6 - https://doi.org/10.1190/INT-2016-0021.1 SN - 2324-8858 SN - 2324-8866 VL - 4 SP - SQ41 EP - SQ49 PB - Society of Exploration Geophysicists CY - Tulsa ER - TY - JOUR A1 - Kamprath, Martin A1 - Mietzner, Dana T1 - The impact of sectoral changes on individual competences: A reflective scenario-based approach in the creative industries JF - Technological forecasting & social change N2 - Many foresight studies concentrate on technological foresight and its impact at the organizational level. However, often these studies overlook the soft factor of employee competences which is critical to adopting technological and organizational changes and to developing the necessary innovation capabilities. This study investigates the theoretical and methodological underdeveloped relationship between technological innovation and social initiated change and the impact on individual competences in a dynamic sector. The setting of our study is the turbulent creative industries as a whole, where creative and artistic expression merges with changing technological progress. In a scenario study we mainly conducted in 2010, we developed a scenario model for competences to combine individual competences with a scenario approach to investigate how competences are important to the sector shift or need to be enhanced in the future. We use primary qualitative data from expert interviews and workshops and secondary data from industry reports to identify thirty-seven influence factors. An influence matrix calculation and a cluster analysis are used to project three different scenarios presenting how future developments of the creative industries will influence the competences needed for creative occupations. Now, five years later, we reflect the accuracy of the developed scenarios via a comparison of today's situation with the situation in the scenarios. We discuss theoretical contributions for the foresight literature and practical implementations for the future of work in general, and in particular for the creative industries case. (C) 2015 Elsevier Inc. All rights reserved. KW - Creative industries KW - Creative economy KW - Digital KW - Convergence KW - Media KW - Entertainment KW - Scenario KW - Foresight KW - Futures studies KW - Technological impact KW - Competences KW - Skills KW - Work KW - Microfoundations KW - Human capital resource KW - Strategic human resources Y1 - 2015 U6 - https://doi.org/10.1016/j.techfore.2015.01.011 SN - 0040-1625 SN - 1873-5509 VL - 95 SP - 252 EP - 275 PB - Elsevier CY - New York ER -