TY - JOUR A1 - Ay-Bryson, Destina Sevde A1 - Weck, Florian A1 - Heinze, Peter Eric A1 - Lang, Thomas A1 - Kühne, Franziska T1 - Can psychotherapy trainees distinguish standardized patients from real patients? T1 - Können Psychotherapeut*innen in Ausbildung standardisierte Patient*innen von realen Patient*innen unterscheiden? BT - a pilot study BT - Ergebnisse einer Pilotstudie JF - Zeitschrift für Klinische Psychologie und Psychotherapie N2 - Background: Under the new psychotherapy law in Germany, standardized patients (SPs) are to become a standard component inpsychotherapy training, even though little is known about their authenticity.Objective:The present pilot study explored whether, followingan exhaustive two-day SP training, psychotherapy trainees can distinguish SPs from real patients. Methods: Twenty-eight psychotherapytrainees (M= 28.54 years of age,SD= 3.19) participated as blind raters. They evaluated six video-recorded therapy segments of trained SPsand real patients using the Authenticity of Patient Demonstrations Scale. Results: The authenticity scores of real patients and SPs did notdiffer (p= .43). The descriptive results indicated that the highest score of authenticity was given to an SP. Further, the real patients did notdiffer significantly from the SPs concerning perceived impairment (p= .33) and the likelihood of being a real patient (p= .52). Conclusions: The current results suggest that psychotherapy trainees were unable to distinguish the SPs from real patients. We therefore stronglyrecommend incorporating training SPs before application. Limitations and future research directions are discussed. N2 - Theoretischer Hintergrund: Mit dem neu eingeführten Direktstudium für zukünftige Psychotherapeut_innen (PiA) wirdder Einsatz von standardisierten Patient_innen (SP) in der Lehre zunehmen, obwohl die Authentizität der Rollendarstellungen durch SPempirisch bislang kaum untersucht wurde. Ziel der vorliegenden Studie war es daher zu untersuchen, ob SP trainiert werden können, dassPsychotherapeut_innen in Ausbildung (PiA) SP von realen Patient_innen nicht unterscheiden können. Methode: Insgesamt nahmen 28 PiA(M= 28.54 Jahre,SD= 3.19) als verblindete Rater teil. Sie haben sechs Therapiesitzungen von trainierten SP und realen Patient_innen mitder Skala Authentizität von Patientendarstellungen bewertet. Ergebnisse: Die Authentizitätswerte von SP unterschieden sich nicht signifi-kant von realen Patient_innen (p= .43). Deskriptive Ergebnisse legen nahe, dass ein SP im Schnitt am authentischsten bewertet wurde.Darüber hinaus unterschieden sich SP und reale Patient_innen nicht hinsichtlich der wahrgenommenen Beeinträchtigung (p= .33) sowie derWahrscheinlichkeit, als reale/r Patient_in bewertet zu werden (p= .52). Fazit: Die vorliegenden Ergebnisse legen nahe, dass PiA SP vonrealen Patient_innen nicht unterscheiden konnten. Daher legen wir ein ausführliches Training der SP nahe, bevor sie für Studium und Lehreeingesetzt werden. Die Limitationen sowie zukünftige Forschungsideen werden diskutiert. KW - evidence-based training KW - learning KW - simulated patients KW - simulation-based KW - education KW - therapist competence KW - evidenzbasiertes Training KW - Lernen KW - Simulationspatient_innen KW - simulationsbasierte Lehre KW - therapeutische KW - Kompetenz Y1 - 2020 U6 - https://doi.org/10.1026/1616-3443/a000594 SN - 1616-3443 SN - 2190-6297 VL - 49 IS - 3 SP - 182 EP - 190 PB - Hogrefe CY - Göttingen ER - TY - JOUR A1 - Koshkina, Olga A1 - Lang, Thomas A1 - Thiermann, Raphael A1 - Docter, Dominic A1 - Stauber, Roland H. A1 - Secker, Christian A1 - Schlaad, Helmut A1 - Weidner, Steffen A1 - Mohr, Benjamin A1 - Maskos, Michael A1 - Bertin, Annabelle T1 - Temperature-Triggered Protein Adsorption on Polymer-Coated Nanoparticles in Serum JF - Langmuir N2 - The protein corona, which forms on the nanoparticle's surface in most biological media, determines the nanoparticle's physicochemical characteristics. The formation of the protein corona has a significant impact on the biodistribution and clearance of nanoparticles in vivo. Therefore, the ability to influence the formation of the protein corona is essential to most biomedical applications, including drug delivery and imaging. In this study, we investigate the protein adsorption on nanoparticles with a hydrodynamic radius of 30 nm and a coating of thermoresponsive poly(2-isopropyl-2-oxazoline) in serum. Using multiangle dynamic light scattering (DLS) we demonstrate that heating of the nanoparticles above their phase separation temperature induces the formation of agglomerates, with a hydrodynamic radius of 1 mu m. In serum, noticeably stronger agglomeration occurs at lower temperatures compared to serum-free conditions. Cryogenic transmission electron microscopy (cryo-TEM) revealed a high packing density of agglomerates when serum was not present. In contrast, in the presence of serum, agglomerated nanoparticles were loosely packed, indicating that proteins are intercalated between them. Moreover, an increase in protein content is observed upon heating, confirming that protein adsorption is induced by the alteration of the surface during phase separation. After cooling and switching the surface back, most of the agglomerates were dissolved and the main fraction returned to the original size of approximately 30 nm as shown by asymmetrical flow-field flow fractionation (AF-FFF) and DLS. Furthermore, the amounts of adsorbed proteins are similar before and after heating the nanoparticles to above their phase-separation temperature. Overall, our results demonstrate that the thermoresponsivity of the polymer coating enables turning the corona formation on nanoparticles on and off in situ. As the local heating of body areas can be easily done in vivo, the thermoresponsive coating could potentially be used to induce the agglomeration of nanopartides and proteins and the accumulation of nanoparticles in a targeted body region. Y1 - 2015 U6 - https://doi.org/10.1021/acs.langmuir.5b00537 SN - 0743-7463 VL - 31 IS - 32 SP - 8873 EP - 8881 PB - American Chemical Society CY - Washington ER - TY - JOUR A1 - Koshkina, Olga A1 - Westmeier, Dana A1 - Lang, Thomas A1 - Bantz, Christoph A1 - Hahlbrock, Angelina A1 - Würth, Christian A1 - Resch-Genger, Ute A1 - Braun, Ulrike A1 - Thiermann, Raphael A1 - Weise, Christoph A1 - Eravci, Murat A1 - Mohr, Benjamin A1 - Schlaad, Helmut A1 - Stauber, Roland H. A1 - Docter, Dominic A1 - Bertin, Annabelle A1 - Maskos, Michael T1 - Tuning the Surface of Nanoparticles: Impact of Poly(2-ethyl-2-oxazoline) on Protein Adsorption in Serum and Cellular Uptake JF - Macromolecular bioscience N2 - Due to the adsorption of biomolecules, the control of the biodistribution of nanoparticles is still one of the major challenges of nanomedicine. Poly(2-ethyl-2-oxazoline) (PEtOx) for surface modification of nanoparticles is applied and both protein adsorption and cellular uptake of PEtOxylated nanoparticles versus nanoparticles coated with poly(ethylene glycol) (PEG) and non-coated positively and negatively charged nanoparticles are compared. Therefore, fluorescent poly(organosiloxane) nanoparticles of 15 nm radius are synthesized, which are used as a scaffold for surface modification in a grafting onto approach. With multi-angle dynamic light scattering, asymmetrical flow field-flow fractionation, gel electrophoresis, and liquid chromatography-mass spectrometry, it is demonstrated that protein adsorption on PEtOxylated nanoparticles is extremely low, similar as on PEGylated nanoparticles. Moreover, quantitative microscopy reveals that PEtOxylation significantly reduces the non-specific cellular uptake, particularly by macrophage-like cells. Collectively, studies demonstrate that PEtOx is a very effective alternative to PEG for stealth modification of the surface of nanoparticles. KW - cellular uptake KW - nanoparticles KW - poly(2-ethyl-2oxazoline) KW - poly(ethylene glycol) KW - protein adsorption Y1 - 2016 U6 - https://doi.org/10.1002/mabi.201600074 SN - 1616-5187 SN - 1616-5195 VL - 16 SP - 1287 EP - 1300 PB - Wiley-VCH CY - Weinheim ER - TY - JOUR A1 - Maiwald, Lisa Marie A1 - Junga, Yvonne Marie A1 - Lang, Thomas A1 - Montini, Romina A1 - Witthöft, Michael A1 - Heider, Jens A1 - Schröder, Annette A1 - Weck, Florian T1 - The role of therapist and patient in-session behavior for treatment outcome in exposure-based cognitive behavioral therapy for panic disorder with agoraphobia JF - Journal of clinical psychology N2 - Objective There is a very limited amount of research on the relationship between therapist and patient in‐session behavior and treatment outcome in cognitive behavioral therapy (CBT) for panic disorder with agoraphobia (PD/AG). Additionally, the findings tend to be inconclusive. This study investigates the association between therapist competence, adherence, patient interpersonal behavior, and therapeutic alliance and outcome in a low‐control CBT setting by using comprehensive measures. Methods Twenty‐six patients with PD/AG received 12 sessions of exposure‐based CBT. With regard to the outcome, treatments were classified either as problematic or nonproblematic by means of distinct criteria. Two raters evaluated the in‐session behavior. Results Patient interpersonal behavior was significantly associated with outcome at follow‐up (r = 0.49). At posttreatment, the correlation did not reach significance ( r = 0.34). Competence, adherence, and alliance were not outcome associated. Conclusion The findings emphasize the need for therapists to pay particular attention to patients’ interpersonal behavior during treatment. KW - interpersonal behavior KW - panic disorder with agoraphobia KW - therapeutic alliance KW - therapist competence KW - treatment outcome Y1 - 2018 U6 - https://doi.org/10.1002/jclp.22738 SN - 0021-9762 SN - 1097-4679 VL - 75 IS - 4 SP - 614 EP - 626 PB - Wiley CY - Hoboken ER - TY - JOUR A1 - Weck, Florian A1 - Grikscheit, Florian A1 - Höfling, Volkmar A1 - Kordt, Anne A1 - Hamm, Alfons O. A1 - Gerlach, Alexander L. A1 - Alpers, Georg W. A1 - Arolt, Volker A1 - Kircher, Tilo A1 - Pauli, Paul A1 - Rief, Winfried A1 - Lang, Thomas T1 - The role of treatment delivery factors in exposure-based cognitive behavioral therapy for panic disorder with agoraphobia JF - Journal of anxiety disorders N2 - Treatment delivery factors (i.e., therapist adherence, therapist competence, and therapeutic alliance) are considered to be important for cognitive behavioral therapy (CBT) for panic disorder and agoraphobia (PD/AG). In the current study, four independent raters conducted process evaluations based on 168 two-hour videotapes of 84 patients with PD/AG treated with exposure-based CBT. Two raters evaluated patients’ interpersonal behavior in Session 1. Two raters evaluated treatment delivery factors in Session 6, in which therapists provided the rationale for conducting exposure exercises. At the 6-month follow-up, therapists’ adherence (r = 0.54) and therapeutic alliance (r = 0.31) were significant predictors of changes in agoraphobic avoidance behavior; therapist competence was not associated with treatment outcomes. Patients’ interpersonal behavior in Session 1 was a significant predictor of the therapeutic alliance in Session 6 (r = 0.17). The findings demonstrate that treatment delivery factors, particularly therapist adherence, are relevant to the long-term success of CBT for PD/AG. KW - Interpersonal behavior KW - Panic disorder with agoraphobia KW - Therapeutic alliance KW - Therapist adherence KW - Therapist competence Y1 - 2016 U6 - https://doi.org/10.1016/j.janxdis.2016.05.007 SN - 0887-6185 SN - 1873-7897 VL - 42 SP - 10 EP - 18 PB - Elsevier CY - Oxford ER -