TY  - JOUR
A1  - Solovyev, Nikolay
A1  - Prakash, N. Tejo
A1  - Bhatia, Poonam
A1  - Prakash, Ranjana
A1  - Drobyshev, Evgenii J.
A1  - Michalke, Bernhard
T1  - Selenium-rich mushrooms cultivation on a wheat straw substrate from seleniferous area in Punjab, India
JF  - Journal of trace elements in medicine and biology
N2  - Intensive rice-wheat cultivation cycle in Northern belt of India in general and in the State of Punjab in particular results in large volumes of straw and other post-harvest residue annually. The agricultural area, bordering the districts of Nawanshahr and Hoshiarpur, is popularly known as the seleniferous belt of India. The agri-residues, generated in seleniferous region of this state, are observed to contain significantly high concentration of selenium (Se). The present study was aimed to evaluate the Se uptake by different mushroom species: Pleurotus sajorcaju, Pleurotus ostreatus, Pleurotus citrinopileatus, Agaricus bisporus, and Volvariella volvacea, cultivated on Se-rich wheat and paddy straw from the seleniferous region. Wheat (Pleurotus species and A. bisporus) and paddy straw (V. volvacea) was inoculated with the mycelium spawn and left for 7-20 days, depending on the species, to grow. Control mushrooms were grown analogously using the agricultural residues from non-seleniferous area of the State of Punjab. All fruiting bodies were collected and analyzed in triplicate. Se was quantified using inductively coupled plasma sector field mass spectrometry. The Se accumulation was high in all species under study, being the highest in A. bisporus (1396 mu g/g vs. 46.8 mu g/g in controls - dry weight) and V. volvacea (231 mu g/g vs. 3.77 mu g/g - dry weight). The observed biological efficiency and total yield for all mushroom species showed good and unaltered productivity in Se-rich conditions, if compared to the controls. The Se-rich mushrooms can be prospective Se-supplements sourcing and biofortified foods, providing readily bioavailable and accessible Se for the diets deficient of this biologically essential element.
KW  - Selenium
KW  - Mushrooms
KW  - Cultivation
KW  - Bioaccumulation
KW  - Seleniferous area
KW  - Supplements
Y1  - 2018
U6  - https://doi.org/10.1016/j.jtemb.2018.07.027
SN  - 0946-672X
VL  - 50
SP  - 362
EP  - 366
PB  - Elsevier
CY  - Jena
ER  - 
TY  - JOUR
A1  - Speckmann, Bodo
A1  - Schulz, Sarah
A1  - Hiller, Franziska
A1  - Hesse, Deike
A1  - Schumacher, Fabian
A1  - Kleuser, Burkhard
A1  - Geisel, Juergen
A1  - Obeid, Rima
A1  - Grune, Tilman
A1  - Kipp, Anna Patricia
T1  - Selenium increases hepatic DNA methylation and modulates one-carbon metabolism in the liver of mice
JF  - The journal of nutritional biochemistry
N2  - The average intake of the essential trace element selenium (Se) is below the recommendation in most European countries, possibly causing sub-optimal expression of selenoproteins. It is still unclear how a suboptimal Se status may affect health. To mimic this situation, mice were fed one of three physiologically relevant amounts of Se. We focused on the liver, the organ most sensitive to changes in the Se supply indicated by hepatic glutathione peroxidase activity. In addition, liver is the main organ for synthesis of methyl groups and glutathione via one-carbon metabolism. Accordingly, the impact of Se on global DNA methylation, methylation capacity, and gene expression was assessed. We observed higher global DNA methylation indicated by LINE1 methylation, and an increase of the methylation potential as indicated by higher S-adenosylmethionine (SAM)/S-adenosylhomocysteine (SAH) ratio and by elevated mRNA expression of serine hydroxymethyltransferase in both or either of the Se groups. Furthermore, increasing the Se supply resulted in higher plasma concentrations of triglycerides. Hepatic expression of glycolytic and lipogenic genes revealed consistent Se dependent up-regulation of glucokinase. The sterol regulatory element-binding transcription factor 1 (Srebf1) was also up-regulated by Se. Both effects were confirmed in primary hepatocytes. In contrast to the overall Se-dependent increase of methylation capacity, the up-regulation of Srebf1 expression was paralleled by reduced local methylation of a specific CpG site within the Srebf1 gene. Thus, we provided evidence that Se-dependent effects on lipogenesis involve epigenetic mechanisms. (C) 2017 The Authors. Published by Elsevier Inc.
KW  - Selenium
KW  - DNA methylation
KW  - Liver
KW  - Lipogenesis
KW  - Srebf1
Y1  - 2017
U6  - https://doi.org/10.1016/j.jnutbio.2017.07.002
SN  - 0955-2863
SN  - 1873-4847
VL  - 48
SP  - 112
EP  - 119
PB  - Elsevier
CY  - New York
ER  -