TY  - JOUR
A1  - Lütkecosmann, Steffi
A1  - Warsinke, Axel
A1  - Tschöpe, Winfried
A1  - Eichler, Rüdiger
A1  - Hanack, Katja
T1  - A novel monoclonal antibody suitable for the detection of leukotriene B4
JF  - Biochemical and biophysical research communications
N2  - Leukotriene B4 as an inflammatory mediator is an important biomarker for different respiratory diseases like asthma, chronic obstructive pulmonary disease or cystic lung fibrosis. Therefore the detection of LTB4 is helpful in the diagnosis of these pulmonary diseases. However, until now its determination in exhaled breath condensates suffers from problems of accuracy. Reasons for that could be improper sample collection and preparation methods of condensates and the lack of consistently assay specificity and reproducibility of the used immunoassay detection system. In this study we describe the development and the characterization of a specific monoclonal antibody (S27BC6) against LTB4, its use as molecular recognition element for the development of an enzyme-linked immunoassay to detect LTB4 and discuss possible future diagnostic applications.
KW  - Leukotriene B4
KW  - Monoclonal antibody
KW  - Immunosensor
KW  - Chronic obstructive pulmonary disease (COPD)
KW  - Hapten
Y1  - 2017
U6  - https://doi.org/10.1016/j.bbrc.2016.11.157
SN  - 0006-291X
SN  - 1090-2104
VL  - 482
IS  - 4
SP  - 1054
EP  - 1059
PB  - Elsevier
CY  - San Diego
ER  - 
TY  - JOUR
A1  - Kuhne, Maren
A1  - Dippong, Martin
A1  - Flemig, Sabine
A1  - Hoffmann, Katrin
A1  - Petsch, Kristin
A1  - Schenk, Jörg A.
A1  - Kunte, Hans-Jörg
A1  - Schneider, Rudolf J.
T1  - Comparative characterization of mAb producing hapten-specific hybridoma cells by flow cytometric analysis and ELISA
JF  - Journal of immunological methods
N2  - A novel method that optimizes the screening for antibody-secreting hapten-specific hybridoma cells by using flow cytometry is described. Cell clones specific for five different haptens were analyzed. We selectively double stained and analyzed fixed hybridoma cells with fluorophore-labeled haptens to demonstrate the target-selectivity, and with a fluorophore-labeled anti-mouse IgG antibody to characterize the level of surface expression of membrane-bound IgGs. ELISA measurements with the supernatants of the individual hybridoma clones revealed that antibodies from those cells, which showed the highest fluorescence intensities in the flow cytometric analysis, also displayed the highest affinities for the target antigens. The fluorescence intensity of antibody-producing cells corresponded well with the produced antibodies' affinities toward their respective antigens. Immunohistochemical staining verified the successful double labeling of the cells. Our method makes it possible to perform a high-throughput screening for hybridoma cells, which have both an adequate IgG production rate and a high target affinity. (C) 2014 Elsevier B.V. All rights reserved.
KW  - Immunization
KW  - Hapten
KW  - Monoclonal antibodies
KW  - Hybridoma
KW  - Flow cytometry
KW  - ELISA
Y1  - 2014
U6  - https://doi.org/10.1016/j.jim.2014.07.004
SN  - 0022-1759
SN  - 1872-7905
VL  - 413
SP  - 45
EP  - 56
PB  - Elsevier
CY  - Amsterdam
ER  -